9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
https://canvas.liberty.edu/courses/318794/quizzes/1674761
1/20
Score for this attempt: 78 out of 80
Submitted Sep 16 at 9pm
This attempt took 62 minutes.
2 / 2 pts
Question 1
Double stranded regions of RNA typically take on a(n):
A-form left-handed helix.
A-form right-handed helix.
Correct!
Correct!
B-form left-handed helix.
B-form right-handed helix.
Z-form left-handed helix.
2 / 2 pts
Question 2
Current estimates indicate that _____% of the human genome is
translated into protein.
less than 0.5
roughly 1.5
Correct!
Correct!
roughly 10
roughly 25
more than 50
2 / 2 pts
Question 3
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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2/20
Incubation of DNA at low pH is MOST likely to cause:
depurination.
Correct!
Correct!
depyrimidination.
mutation of cytosine to uracil.
hydrolysis of the phosphodiester bond.
deamination of adenine and guanine.
2 / 2 pts
Question 4
To make an DNA copy of a RNA sequence, you need the reverse
transcriptase enzyme and an oligonucleotide primer made of which
nucleotide?
deoxyadenosine
deoxythymine
Correct!
Correct!
deoxycytosine
deoxyuridine
deoxyguanosine
2 / 2 pts
Question 5
When performing PCR, what is the advantage to using a DNA
polymerase isolated from a thermophilic organism?
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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The PCR reaction does not need to be cooled below the
denaturation step.
The PCR reaction does not need primers to proceed.
The PCR reaction does not need to have fresh enzyme added
after each cycle.
Correct!
Correct!
The PCR reaction does not need to have fresh dNTPs added
after each cycle.
The PCR reaction does not need to have fresh template DNA
added after each cycle.
2 / 2 pts
Question 6
Certain restriction enzymes produce cohesive (sticky) ends. This means
that they:
cut both DNA strands at the same base pair.
cut in regions of high GC content, leaving ends that can form
more hydrogen bonds than ends of high AT content.
make a staggered double-strand cut, leaving ends with a few
nucleotides of single-stranded DNA protruding.
Correct!
Correct!
make ends that can anneal to cohesive ends generated by any
other restriction enzyme.
stick tightly to the ends of the DNA they have cut.
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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2 / 2 pts
Question 7
Compounds that generate nitrous acid (such as nitrites, nitrates, and
nitrosamines) change DNA molecules by:
breakage of phosphodiester bonds.
deamination of bases.
Correct!
Correct!
depurination.
formation of thymine dimers.
transformation of A T.
2 / 2 pts
Question 8
What would happen if blocking tags were NOT used in reversible
terminator sequencing, a next-generation method of DNA sequencing?
The DNA fragments to sequence/read would be too large.
More than one dNTP could be incorporated to the same growing
nucleotide chain during a single cycle.
Correct!
Correct!
The flow cell could not immobilize DNA fragments.
Multiple fluorescent colors could be observed across the flow
cell at the same time.
Luciferase would not react in the presence of ATP.
2 / 2 pts
Question 9
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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5/20
Which tag is NOT used to study protein function?
green fluorescent protein (GFP)
synteny tag
Correct!
Correct!
tandem affinity purification (TAP)
Gal4p DNA binding domain
Gal4p activation domain
2 / 2 pts
Question 10
A major component of RNA but not of DNA is:
adenine.
cytosine.
guanine.
thymine.
uracil.
Correct!
Correct!
2 / 2 pts
Question 11
A tetraplex of DNA is MOST likely to form with which residue?
Adenosine
Thymidine
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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Guanosine
Correct!
Correct!
Cytidine
A tetraplex is equally likely with all bases.
2 / 2 pts
Question 12
Which repeating sequences in the human genome are the targets of
technologies used in forensic DNA analysis?
simple-sequence repeats
single nucleotide polymorphisms
introns
short tandem repeats
Correct!
Correct!
long repetitive sequences
2 / 2 pts
Question 13
What is the MOST likely length of an mRNA that will code for a
polypeptide with 150 amino acids?
50 nucleotides
150 nucleotides
450 nucleotides
>450 nucleotides
Correct!
Correct!
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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All of these lengths are possible depending on the organism in
which the mRNA is found.
2 / 2 pts
Question 14
In DNA sequencing by the Sanger (dideoxy) method:
radioactive dideoxy ATP is included in each of four reaction
mixtures before enzymatic synthesis of complementary strands.
specific enzymes are used to cut the newly synthesized DNA
into small pieces, which are then separated by electrophoresis.
the dideoxynucleotides must be present at high levels to obtain
long stretches of DNA sequence.
the role of the dideoxy CTP is to occasionally terminate
enzymatic synthesis of DNA where Gs occur in the template
strands.
Correct!
Correct!
the template DNA strand is radioactive.
2 / 2 pts
Question 15
What is a significant problem with regard to making cDNA libraries to
analyze overall gene function in an eukaryotic organism?
A cDNA library will only contain genes that are actively
transcribed in the organism.
Correct!
Correct!
Reverse transcriptase cannot be used to make cDNA libraries.
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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cDNA cannot be amplified by PCR.
cDNA libraries do not contain intron sequences.
cDNA libraries may contain several alternatively spliced forms
of the same gene.
2 / 2 pts
Question 16
The biochemical activity of a protein, such as its enzymatic activity, is
called its _____ function.
phenotypic
genotypic
cellular
molecular
Correct!
Correct!
organismal
2 / 2 pts
Question 17
In the Watson-Crick model of DNA structure (now called B-form DNA):
a purine in one strand always hydrogen bonds with a purine in
the other strand.
A–T pairs share three hydrogen bonds.
G–C pairs share two hydrogen bonds.
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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the 5' ends of both strands are at one end of the helix.
the bases occupy the interior of the helix.
Correct!
Correct!
2 / 2 pts
Question 18
The phosphodiester bond that joins adjacent nucleotides in DNA:
associates ionically with metal ions, polyamines, and proteins.
Correct!
Correct!
is positively charged.
is susceptible to alkaline hydrolysis.
Links C-2 of one base to C-3 of the next.
links C-3 of deoxyribose to N-1 of thymine or cytosine.
2 / 2 pts
Question 19
If you wanted to clone a large fragment of DNA into a unicellular
eukaryotic host, what would you use?
a bacmid
a plasmid
a bacterial artificial chromosome
a yeast artificial chromosome
Correct!
Correct!
a virus
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Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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10/20
2 / 2 pts
Question 20
Which DNA sequence is a palindrome?
AATGCC
GGATCC
Correct!
Correct!
GATATG
CCCGCG
AGTAGT
2 / 2 pts
Question 21
The covalent backbone of DNA and RNA consists of:
alternating phosphate groups and nitrogenous bases.
alternating phosphate groups and pentose residues.
Correct!
Correct!
alternating pentose residues and nitrogenous bases.
alternating pentose residues and cyclic nucleosides.
None of the answers is correct.
2 / 2 pts
Question 22
DNA is _____ chemically stable than RNA due to the _____ on _____.
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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more; 2 -hydroxyl groups; RNA
Correct!
Correct!
more; 2 -hydroxyl groups; DNA
more; 3 -hydroxyl groups; RNA
less; 2 -hydroxyl groups; RNA
less; 2 -hydroxyl groups; DNA
2 / 2 pts
Question 23
In a double-stranded nucleic acid, cytosine typically base-pairs with:
adenosine.
guanine.
Correct!
Correct!
inosine.
thymine.
uracil.
2 / 2 pts
Question 24
Which compound is NOT needed to build a cDNA library?
genomic DNA
Correct!
Correct!
mRNA
reverse transcriptase
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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dNTPs
DNA polymerase
0 / 2 pts
Question 25
The process of linkage analysis does NOT rely on which technique to
determine the genetic causes of diseases?
analysis of family pedigrees
identification of SNPs that are most often inherited with the
disease-causing gene
You Answered
You Answered
the sequence of the entire chromosome where the disease-
causing gene is located to directly identify it
Correct Answer
Correct Answer
comparing the DNA of people who have the disease with the
DNA of people who don't have the disease
obtaining many sequences of a region of the chromosome that is
associated with the disease
2 / 2 pts
Question 26
Approximately how much of the human genome encodes the exons of
protein-coding sequences?
1.5%
Correct!
Correct!
5%
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Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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30%
100%
80%
2 / 2 pts
Question 27
In the Watson-Crick model for the DNA double helix (B form) the A–T
and G–C base pairs share which property?
The distance between the two glycosidic (base-sugar) bonds is
the same in both base pairs, within a few tenths of an angstrom.
Correct!
Correct!
The molecular weights of the two base pairs are identical.
The number of hydrogen bonds formed between the two bases
of the base pair is the same.
The plane of neither base pair is perpendicular to the axis of the
helix.
The proton-binding groups in both base pairs are in their
charged or ionized form.
2 / 2 pts
Question 28
Double-stranded regions of RNA:
are less stable than double-stranded regions of DNA.
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can be observed in the laboratory, but probably have no
biological relevance.
can form between two self-complementary regions of the same
single strand of RNA.
Correct!
Correct!
do not occur.
have the two strands arranged in parallel (unlike those of DNA,
which are antiparallel).
2 / 2 pts
Question 29
A pyrimidine is joined covalently to the pentose sugar through the:
N-1 of the base to the 1 carbon of the pentose.
Correct!
Correct!
N-9 of the base to the 1 carbon of the pentose.
N-1 of the base to the 5 carbon of the pentose.
N-9 of the base to the 5 carbon of the pentose.
None of the answers is correct.
2 / 2 pts
Question 30
A method of determining if proteins are interacting in vivo that makes
use of a transcription factor involved in regulating galactose metabolism
genes is:
a DNA microarray.
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Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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yeast two-hybrid analysis.
Correct!
Correct!
tandem affinity purification.
immunofluorescence.
quantitiative PCR.
2 / 2 pts
Question 31
The gene for green fluorescent protein was originally isolated from which
species?
a panda
a firefly
a mushroom
a jellyfish
Correct!
Correct!
a glowworm
2 / 2 pts
Question 32
If you want to determine what other proteins might be interacting with
the protein you have been studying, what type of fusion tag would you
add to your protein to aid in purification of both the protein you have
been studying and the proteins interacting with it?
a green fluorescent protein fusion tag
a tandem affinity purification tag
Correct!
Correct!
a maltose-binding protein tag
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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an epitope tag
a -galactosidase tag
2 / 2 pts
Question 33
The phosphodiester bonds that link adjacent nucleotides in both RNA and
DNA:
always link A with T and G with C.
are susceptible to alkaline hydrolysis.
are uncharged at neutral pH.
form between the planar rings of adjacent bases.
join the 3' hydroxyl of one nucleotide to the 5' hydroxyl of the
next.
Correct!
Correct!
2 / 2 pts
Question 34
The difference between thymine and uracil is:
one methylene group on the pyrimidine ring.
one methyl group on the pyrimidine ring.
Correct!
Correct!
one hydroxyl group on the ribose ring.
one amine group on the pyrimidine ring.
one methyl group on the purine ring.
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Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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2 / 2 pts
Question 35
Which method of visualizing a protein's location should you use if you
want to determine its location in a living cell?
a GFP fusion tag
Correct!
Correct!
immunofluorescence
qPCR
Gram stain
affinity purification
2 / 2 pts
Question 36
Common problems with expression of eukaryotic proteins in bacteria
may include proteins:
aggregating into inclusion bodies.
not folding correctly due to absence of chaperones.
not undergoing posttranslational modification.
All of the answers are correct.
Correct!
Correct!
None of the answers is correct.
2 / 2 pts
Question 37
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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18/20
In living cells, nucleotides and their derivatives can serve as:
carriers of metabolic energy.
enzyme cofactors.
intracellular signals.
precursors for nucleic acid synthesis.
All of the answers are correct.
Correct!
Correct!
2 / 2 pts
Question 38
Especially stable haplotypes exist in which portions of the human
genome due to their relative lack of meiotic recombination?
the Y chromosome
the X chromosome
the mitochondrial genome
both the Y chromosome and the mitochondrial genome
Correct!
Correct!
both the X chromosome and the mitochondrial genome
2 / 2 pts
Question 39
Based on Chargaff's rules, which base compositions for double-stranded
DNA are possible?
%A %G %C %T %U
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
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5 45 45 5 0
Correct!
Correct!
20 20 20 20 20
35 15 35 15 0
All of the answers are correct.
None of the answers is correct.
2 / 2 pts
Question 40
In a nucleoside, the pentose sugar is in its _____ form, and the ring is
_____.
-furanose; planar
-pyranose; puckered
-furanose; planar
-furanose; puckered
Correct!
Correct!
-pyranose; planar
9/16/22, 9:01 PM
Quiz: Basic Concepts of Genomics: NUTR641: Nutritional Biochemistry I (B01)
https://canvas.liberty.edu/courses/318794/quizzes/1674761
20/20
Quiz Score: 78 out of 80
