Preparation and manufacture of medium
Media function
Media is a substrate needed to grow and breed microorganisms. Before being
used in experiments, this media needs to be sterilized
first, so that it is not overgrowned by unwanted microorganisms
(contaminants). In order for the microbes that we culture can grow well, then
the requirements that must be met by a media are:
a. In it must be contained the ingredients needed by the microbes that will
grown. These materials include macro elements, micro elements, and trace
elements and regulatory substances grow.
breakfast. The media must have osmosis pressure, surface voltage, and pH
In accordance with the need for microbes that will be cultured.
C.. The media must be sterile before being used to foster the microbes
required.
Media type
According to the material used in making, the media can
Classed into:
a. Natural media: the media that the forming component consists of natural
ingredients,
Like potatoes, bean sprouts, meat, rice, and so on.
breakfast. Semi synthetic media: the media that the forming material consists of
a mixture of natural ingredients and synthetic materials. Example: order bean
sprouts, so that dextrose potatoes, etc.
C.. Synthetic media: media whose overall shaper material is made of
Synthetic ingredients. Example: so that Sabouraud, Endo Agar, so that Czapex
Dox, etc.
According to its form, the media can be classified into:
a. Liquid media: media that is not added to the compactor (agar), so this media
In a runny (liquid) state. Example: Lactose Broth, Nutrient Broth.
breakfast. Semi-solid media: media containing the same material as liquid
media,
But plus a little so that (half concentration so that it is rather crowded.
This media is used to grow microbes that require many water and
Live in an anaerobic environment or facultative anaerob. This media is also
used
For motility testing a bacterium.
C.. Solid media: liquid media added with gelatin so that it becomes solid.
Example: Nutrient Agar (NA), Potato Dextrose Agar (PDA), etc.
According to the use, the media is classified into:
a. General Media: Media used to grow one or more groups
Microbes in general. Example: Nutrient Agar (Media to Grow Groups
bacteria, potato dextrose so that (media used to grow groups
mushrooms, etc.
breakfast. Add-ons Media: Media used to fertilize certain microbes before
grown on the media used in the study. Example: Selenit Broth (for
Fertilize the growth of salmonella bacteria.
C.. Selective media: media used to grow certain species from
Microbes, by inhibiting other species growth that is not desirable.
Example: SS media so that (salmonella and shigella agar) for salmonella bacteria
and
Shigella.
d. Media Calculation: Media used to calculate a microbial amount
materials. This media can be in the form of general media and selective media.
Media making
In this lab, you will make several kinds of media, namely the media
Nutrient Agar, Malt Extract, Lactose Broth, Brilliant Green 2% Bile Broth, and
Media
Endo so that. All these media are synthetic media that are sold in
Instant form, so in making it, you just pay attention to the instructions
What is printed on every media.
How to make nutrient agar.
To make this 1 liter of medium, do the following procedure:
a. Weigh 23.5 grams of instant medium.
breakfast. Suspension in aquadest and the final volume is made 1000 ml.
C.. Heat this suspension until it is cooked.
d. Put in a test tube (5ml for tilt media, and 10 ml for
upright media).
Hey. Sterilize with Autoclave at 121 OC temperatures and 15 lbs pressure. For 15
minutes.
f. Save in a cool and dry place (in the refrigerator).
Make Extract Malt
To make this medium 1 liter, do the following steps:
a. Weigh as many as the instant media gram.
breakfast. Suspension in aquimadest until the final volume becomes 1000 ml.
C.. Put in a test tube (approximately 7 ml of each tube)
d. sterilize with autoclave at 121 oC and a pressure of 15 lbs, for 15 minutes
Making Lactose Broth Medium
In this lactose broth medium practicum will be made with two concentrations
(Single
Strength and double strength). To make a single liter of Lactose Broth's single
strength,
Do the following procedure:
a. Weighing 13 grams of medium lactose broth instant.
breakfast. Suspension in distilled water until the final volume reaches 1000 ml.
C.. Enter into a test tube that has been equipped with Durham tube.
d. Sterilize with autoclave at 121 oC at a pressure of 15 lbs for 15 minutes.
Hey. Save in a cool and dry place (in the refrigerator).
f. The same procedure is done to make a double strength lactose broth, but
The concentration is folded.
Manufacture of BGBB Medium (Brilliant Green Bile 2% Broth)
To make this medium liter, do the following procedure:
a. Weigh as much as the instant medium gram.
breakfast. Suspension in the aquadest and the final volume is made to 1000 ml.
C.. Put it in a small reaction tube that has been equipped with a durham tube.
d. Sterilize with autoclave at a temperature of 121 oC at a pressure of 15 lbs, for
15
minutes.
Hey. Store in a cool and dry place.
Medium Endo Making Agar
To make this 1 liter of medium, do the following procedure:
a. Weigh as much as the instant medium gram.
breakfast. Suspension in distilled water and make the final volume to 1000 ml.
C.. Heat until all components dissolve.
d. Sterilize with autoclave at 121 oC at a 15 lbs pressure, for 15 minutes.
Hey. Store in a cool and dry place (in the refrigerator).
Manufacture of medium palte count agar
To make this 1 liter of medium, do the following procedure:
a. Weigh 23.5 grams of PCA instant medium, pronadisa).
Note:
Different brands have different composition so that gram weight / lt
Maybe it's different. So, pay attention to the written guide on the packaging /
bottle
Media that is used.
breakfast. Suspension in distilled water and make the final volume to 1000 ml.
C.. Heat until all soluble components (this step can be done if not
Prepare for the heart / so that Mirin in the test tube).
d. Sterilize with autoclave at 121 oC at a 15 lbs pressure, for 15 minutes.
Hey. The media can be poured into the Petri dish that has been autovlave (see
picture on
below).
For one cup of 9.9mm diameter, a thickness of 1.5 cm, the volume so that
Increased by 17.5-20 ml for the media thickness so that it is good.
In order to be in the bone into Petri's dish in the media temperature condition +
65-75oC. At temperature at
Down it to be my member.
f. Let it be in the petri dish to freeze, the cup lid is open until so
Memrkku (20-30 minutes in laminar flow).
g. Store in a cool and dry place (in the refrigerator) in a state of wrapped
plastic bag and to be placed upside down (the cover of the petri cup below and
In order above).
Media is generally made in the form of media so that the media
poured in the Petri dish, the media to slant / so that it is upright prepared in
The reasons of the reasons are used to store culture. Usually aerobic culture
stored in to be tilted, aside anaerobal culture is generally stored in agar
stab (stab) on the media upright.
