introduction/background Bio lab paper

Introduction/background lab paper (2 and 1/2 pages) about the following; explaining what are these techniques? What each is used for; functions? Why is it important? Use the references in the attachment and first of all check the instruction file !!

1. ORF fusions between GFP and a Target Gene

2. pPEM109

3. LEPA/EF4; elongation factor 4 (EF-4) refer to the gene as lepA 

4. Tn5 Vitro transposition reaction: http://www.jbc.org/content/273/13/7367.full

5. TRANSFORMATION OF TRANSPOSITION MIXTURE INTO E. coli.