Quiz: Basic Concepts of Genetic
Expression
Due Sep 24 at 11:59pm
Points 80
Questions 40
Time Limit 90 Minutes
Allowed Attempts 2
Instructions
The quiz:
Covers the Textbook material from Module 5: Week 5.
Contains 40 multiple-choice questions.
Is limited to 90 minutes
Allows 2 attempts.
Is worth 80 points.
Submit this assignment by 11:59 p.m. (ET) on Sunday of Module 5: Week 5.
TAKE THE QUIZ AGAIN
Attempt History
Attempt Time Score
LATEST 84 minutes 78 out of 80
Score for this attempt: 78 out of 80
Submitted Sep 13 at 1:52pm
This attempt took 84 minutes.
Question 1
2 / 2 pts
In living organisms, genetic information is most often stored in the form of
________.
ribonucleic acid
enzymes
C !
deoxyribonucleic acid
deoxynucleotides
proteins
Question 2
2 / 2 pts
What reagents are required to perform PCR?
DNA fragment, primers flanking the region of interest, dNTPs, ddNTPs, DNA
polymerase
DNA fragment, primers flanking the region of interest, dNTPs, DNA
endonuclease
DNA fragment, one primer, dNTPs, DNA polymerase, DNA endonuclease
DNA fragment, one primer, dNTPs, DNA polymerase, DNA ligase
C !
DNA fragment, primers flanking the region of interest, dNTPs, DNA
polymerase
Question 3
2 / 2 pts
The DNA strand that serves as the template for the synthesis of RNA is often
called the ________.
transcription strand
transfer strand
coding strand
C !
noncoding strand
messenger strand
Question 4
2 / 2 pts
Which of the following is correctly paired with its function?
RNase H: removal of RNA primer
helicase: creates single strand DNA
primase: production of an RNA primer for DNA polymerase
DNA ligase: joins the discontinuous segments on the lagging strand
C !
all of the above
Question 5
2 / 2 pts
In DNA, the ribose derivative lacks an ________ on C-________.
amine; 3
amine; 2
alcohol; 3
C !
alcohol; 2
none of the above
Question 6
2 / 2 pts
Which of the following is thought to be the origin of RNA interference?
removal of mistranscribed mRNA
removal of excessive mRNA
antibiotic for RNA based bacteria
C !
antiviral defense
none of the above
Question 7
2 / 2 pts
Genes that are not often transcribed generally have ________ sequences that
bind to ________ proteins.
inhibitor; mediator
negative control; arrestor
C !
silencer; repressor
interrupter; regulatory
obstruction; restraint
Question 8
2 / 2 pts
A gene knockout is ________.
a gene present on a YAC
C !
a gene that has been inactivated or removed from an organism
a dominant gene that knocks out expression of other genes
a gene inserted in place of another gene
none of the above
Question 9
2 / 2 pts
Without telomeres, what would happen to the ends of eukaryotic
chromosomes during replication?
they would be replicated just like the rest of the chromosome
they would be lengthened
they would be mutated
C !
they would be shortened
none of the above
Question 10
2 / 2 pts
Which of the following represents the correct order of steps in a PCR reaction
beginning with double-stranded DNA?
strand separation, primer extension, primer annealing
C !
strand separation, primer annealing, primer extension
primer annealing, strand separation, primer extension
primer extension, primer annealing, strand separation
primer annealing, primer extension, strand separation
Question 11
2 / 2 pts
The protein that is responsible for bringing together proteins bound to
enhancers with proteins bound to promoters is called ________.
chromatin remodeling enhancer
σ subunit of RNA polymerase
C !
mediator complex
TAF1
none of the above
Question 12
2 / 2 pts
Because of the high temperature ________ step during a PCR reaction, the
DNA polymerase from ________ is used.
primer extension; bacteriophage λ
primer extension; E. coli
strand separation; E. coli
C !
strand separation; Thermus aquaticus
primer annealing; Thermus aquaticus
Question 13
2 / 2 pts
What type of modification is used to make CpG islands?
methylation of guanine
acetylation of guanine
phosphorylation of guanine
acetylation of cytosine
C !
methylation of cytosine
Question 14
2 / 2 pts
An E. coli has ________ replication fork(s) on its single chromosome; humans
have ________ replication fork(s) on each chromosome.
many; many
1; 2
C !
2; many
2; 2
1; 1
Question 15
2 / 2 pts
What does allolactose bind?
C !
lac repressor
promoter
lac operon
operator
all of the above
Question 16
2 / 2 pts
Which of the following is the major factor in determining how quickly an
mRNA is degraded?
degree of intron removal
C !
length of poly(A) tail
overall length of the mRNA
how quickly the cap is removed
all of the above play a role
Question 17
2 / 2 pts
All cellular RNAs are transcribed from ________.
C !
DNA templates
RNA templates
either DNA or RNA templates, but not both in the same organism
a combination of DNA and RNA templates
none of the above
Question 18
0 / 2 pts
Synthesis of telomeres requires the enzyme ________, which is a type of
________.
Correct Answer
telomerase; reverse transcriptase
ribonuclease; RNA-specific nuclease
telomerase; helicase
ribotelomerase; ligase
Y Answered
none of the above (correct answer)
Question 19
2 / 2 pts
What enzyme is required to form a new phosphodiester bond when inserting
DNA into a plasmid?
C !
ligase
endonuclease
DNA polymerase
exonuclease
clonase
Question 20
2 / 2 pts
Which of the following allows a gene to give more than one viable protein
product?
use of RNA interference
action of the decapping enzyme
C !
alternative splicing
exon suppression
frame shift mutations
Question 21
2 / 2 pts
The DNA strand that serves as the template for the synthesis of RNA is often
called the ________.
C !
noncoding strand
coding strand
transcription strand
transfer strand
messenger strand
Question 22
2 / 2 pts
What sugar is metabolized by the enzymes under the control of
the lac operon?
C !
lactose
sucrose
maltose
mannose
galactose
Question 23
2 / 2 pts
The most prevalent mode of DNA-protein interaction is achieved with a(n)
________ that binds to the ________ groove of DNA.
β-sheet; minor
β-turn; major
α-helix; minor
C !
α-helix; major
β-sheet; major
Question 24
2 / 2 pts
Which strand of DNA is replicated exclusively in a discontinuous fashion?
forward strand
leading strand
the strand that is read in a 5′ to 3′ direction
reverse strand
C !
lagging strand
Question 25
2 / 2 pts
Separation of DNA into a single stranded region around RNA polymerase
creates ________.
hybridized DNA
Z-DNA
C !
a transcription bubble
DNA bound to single stranded binding proteins
none of the above
Question 26
2 / 2 pts
Addition of a(n) ________ group to certain lysine residues of a histone protein
makes the region of chromatin transcriptionally ________; addition of a(n)
________ group to certain lysine residues makes the region of chromatin
transcriptionally ________.
methyl; silent; phosphate; active
acetyl; silent; methyl; active
methyl; highly active; acetyl; active
phosphate; silent; methyl; silent
C !
methyl; silent; acetyl; active
Question 27
2 / 2 pts
What is the region immediately adjacent to the starting site for RNA
polymerization called?
enhancer
initiator
TF binding site
consensus sequence
C !
promoter
Question 28
2 / 2 pts
Histone proteins that bind to areas of DNA that are highly transcribed are
often ________ on ________ residues.
phosphorylated; tyrosine
C !
acetylated; lysine
phosphorylated; serine
acetylated; histidine
methylated; lysine
Question 29
2 / 2 pts
How do the NTPs enter RNA polymerase?
from the exit site, as the DNA leaves the enzymes, the NTPs move in the
opposite direction
C !
through a special channel sized just for NTPs
base-paired with the incoming (downstream) DNA
with the incoming DNA but not base-paired with it
there is no specific way, any of the above routes are used
Question 30
2 / 2 pts
Which of the following best describes the functioning of the lac repressor
protein?
in the presence of allolactose, the protein binds the operator inhibiting
transcription
C !
in the absence of allolactose, the protein binds the operator inhibiting
transcription
in the absence of allolactose, the protein binds the operator enhancing
transcription
in the presence of allolactose, the protein binds the operator enhancing
transcription
none of the above
Question 31
2 / 2 pts
In most organisms, replication proceeds in a ________ manner from the
________.
bidirectional; lagging strand
C !
bidirectional; replication origin
bidirectional; theta site
unidirectional; chromosome ends
none of the above
Question 32
2 / 2 pts
In a typical gene, what percentage of the bases represents introns?
50%
10%
C !
90%
5%
25%
Question 33
2 / 2 pts
Which type of DNA repair is used to remove and replace a single modified
base that cannot be converted back to the normal base by a direct repair
process?
mismatch repair
DNA photolyase
nucleotide excision repair
C !
base excision repair
none of the above
Question 34
2 / 2 pts
What is the process used for genes shuffling in meiosis that is also used to
repair double-strand breaks?
nonhomologous end-joining
telomerase-assisted ligation
non-mutagenic strand coupling
homologous end-joining
C !
recombination
Question 35
2 / 2 pts
What technique involves addition of primers that do not exactly match the
sequence of a gene, thus allowing for introduction of a mutation?
transformation
selection
cloning
C !
site-directed mutagenesis
none of the above
Question 36
2 / 2 pts
What is the purpose of DNA helicase?
removal of negative supercoiling to allow for replication
C !
separation of double stranded DNA into single stranded DNA in front of DNA
polymerase
conversion of single stranded DNA to double stranded DNA behind DNA
polymerase
removal of positive supercoiling to allow for replication
none of the above
Question 37
2 / 2 pts
What does a reverse transcriptase do?
converts a DNA genome into an RNA genome
replicates DNA in a 3′ to 5′ fashion
replicates RNA in a 3′ to 5′ fashion
replicates RNA in a 5′ to 3′ fashion
C !
converts an RNA genome into a DNA genome
Question 38
2 / 2 pts
Which of the following explains why circular DNA, such as the chromosomes
of some viruses and bacteria, appears to be twisted?
circular DNA is Z-form
viral and bacterial DNA is always single stranded
C !
supercoiling
hybridization
none of the above
Question 39
2 / 2 pts
Double-stranded DNA molecules can be cleaved at specific recognition sites
by ________.
reverse transcriptase
RNA polymerase
DNA ligase
DNA polymerase
C !
Type II restriction endonucleases
Question 40
2 / 2 pts
During termination, Rho acts as a ________.
ligase
NTP hydrolase
single-strand binding protein
C !
helicase
polymerase
