Bacteria - Cell Walls
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
It is important to note that not all bacteria have a cell wall. Having said that though, it is also
important to note that most bacteria (about 90%) have a cell wall and they typically have one
of two types: a gram positive cell wall or a gram negative cell wall. The two different cell
wall types can be identified in the lab by a differential stain known as the Gram stain.
Developed in 1884, it’s been in use ever since. Originally, it was not known why the Gram
stain allowed for such reliable separation of bacterial into two groups. Once the electron
microscope was invented in the 1940s, it was found that the staining difference correlated with
differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
After this stain technique is applied the gram positive bacteria will stain purple, while the gram
negative bacteria will stain pink.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Peptidoglycan Structure.
Gram Positive Cell walls
The cell walls of gram positive bacteria are composed predominantly of peptidoglycan. In fact,
peptidoglycan can represent up to 90% of the cell wall, with layer after layer forming around
the cell membrane. The NAM tetrapeptides are typically cross-linked with a peptide interbridge
and complete cross-linking is common. All of this combines together to create an incredibly
strong cell wall.
The additional component in a gram positive cell wall is teichoic acid, a glycopolymer, which
is embedded within the peptidoglycan layers. Teichoic acid is believed to play several
important roles for the cell, such as generation of the net negative charge of the cell, which is
essential for development of a proton motive force. Teichoic acid contributes to the overall
rigidity of the cell wall, which is important for the maintenance of the cell shape, particularly
in rod-shaped organisms. There is also evidence that teichoic acids participate in cell division,
by interacting with the peptidoglycan biosynthesis machinery. Lastly, teichoic acids appear to
play a role in resistance to adverse conditions such as high temperatures and high salt
concentrations, as well as to β-lactam antibiotics. Teichoic acids can either be covalently linked
to peptidoglycan (wall teichoic acids or WTA) or connected to the cell membrane via a lipid
anchor, in which case it is referred to as lipoteichoic acid.
Since peptidoglycan is relatively porous, most substances can pass through the gram positive
cell wall with little difficulty. But some nutrients are too large, requiring the cell to rely on the
use of exoenzymes. These extracellular enzymes are made within the cell’s cytoplasm and then
secreted past the cell membrane, through the cell wall, where they function outside of the cell
to break down large macromolecules into smaller components.
Gram Negative Cell Walls
The cell walls of gram negative bacteria are more complex than that of gram positive bacteria,
with more ingredients overall. They do contain peptidoglycan as well, although only a couple
of layers, representing 5-10% of the total cell wall. What is most notable about the gram
negative cell wall is the presence of a plasma membrane located outside of the peptidoglycan
layers, known as the outer membrane. This makes up the bulk of the gram negative cell wall.
The outer membrane is composed of a lipid bilayer, very similar in composition to the cell
membrane with polar heads, fatty acid tails, and integral proteins. It differs from the cell
membrane by the presence of large molecules known as lipopolysaccharide (LPS), which are
anchored into the outer membrane and project from the cell into the environment. LPS is made
up of three different components: 1) the O-antigen or O-polysaccharide, which represents the
outermost part of the structure , 2) the core polysaccharide, and 3) lipid A, which anchors the
LPS into the outer membrane. LPS is known to serve many different functions for the cell, such
as contributing to the net negative charge for the cell, helping to stabilize the outer membrane,
and providing protection from certain chemical substances by physically blocking access to
other parts of the cell wall. In addition, LPS plays a role in the host response to pathogenic
gram negative bacteria. The O-antigen triggers an immune response in an infected host, causing
the generation of antibiotics specific to that part of LPS (think of E. coli O157). Lipid A acts
as a toxin, specifically an endotoxin, causing general symptoms of illness such as fever and
diarrhea. A large amount of lipid A released into the bloodstream can trigger endotoxic shock,
a body-wide inflammatory response which can be life-threatening.
The outer membrane does present an obstacle for the cell. While there are certain molecules it
would like to keep out, such as antibiotics and toxic chemicals, there are nutrients that it would
like to let in and the additional lipid bilayer presents a formidable barrier. Large molecules are
broken down by enzymes, in order to allow them to get past the LPS. Instead of exoenzymes
(like the gram positive bacteria), the gram negative bacteria utilize periplasmic enzymes that
are stored in the periplasm. Where is the periplasm, you ask? It is the space located between
the outer surface of the cell membrane and the inner surface of the outer membrane, and it
contains the gram negative peptidoglycan. Once the periplasmic enzymes have broken
nutrients down to smaller molecules that can get past the LPS, they still need to be transported
across the outer membrane, specifically the lipid bilayer. Gram negative cells utilize porins,
which are transmembrane proteins composed of a trimer of three subunits, which form a pore
across the membrane. Some porins are non-specific and transport any molecule that fits, while
some porins are specific and only transport substances that they recognize by use of a binding
site. Once across the outer membrane and in the periplasm, molecules work their way through
the porous peptidoglycan layers before being transported by integral proteins across the cell
membrane.
The peptidoglycan layers are linked to the outer membrane by the use of a lipoprotein known
as Braun’s lipoprotein (good ol’ Dr. Braun). At one end the lipoprotein is covalently bound to
the peptidoglycan while the other end is embedded into the outer membrane via its polar head.
This linkage between the two layers provides additional structural integrity and strength.
Unusual and Wall-less Bacteria
Having emphasized the important of a cell wall and the ingredient peptidoglycan to both the
gram positive and the gram negative bacteria, it does seem important to point out a few
exceptions as well. Bacteria belonging to the phylum Chlamydiae appear to lack
peptidoglycan, although their cell walls have a gram negative structure in all other regards (i.e.
outer membrane, LPS, porin, etc). It has been suggested that they might be using a protein layer
that functions in much the same way as peptidoglycan. This has an advantage to the cell in
providing resistance to β-lactam antibiotics (such as penicillin), which attack peptidoglycan.
Bacteria belonging to the phylum Tenericutes lack a cell wall altogether, which makes them
extremely susceptible to osmotic changes. They often strengthen their cell membrane
somewhat by the addition of sterols, a substance usually associated with eukaryotic cell
membranes. Many members of this phylum are pathogens, choosing to hide out within the
protective environment of a host.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
Overview of Bacterial Cell Walls
A cell wall, not just of bacteria but for all organisms, is found outside of the cell membrane.
It’s an additional layer that typically provides some strength that the cell membrane lacks, by
having a semi-rigid structure.
Both gram positive and gram negative cell walls contain an ingredient known
as peptidoglycan (also known as murein). This particular substance hasn’t been found
anywhere else on Earth, other than the cell walls of bacteria. But both bacterial cell wall types
contain additional ingredients as well, making the bacterial cell wall a complex structure
overall, particularly when compared with the cell walls of eukaryotic microbes. The cell walls
of eukaryotic microbes are typically composed of a single ingredient, like the cellulose found
in algal cell walls or the chitin in fungal cell walls.
The bacterial cell wall performs several functions as well, in addition to providing overall
strength to the cell. It also helps maintain the cell shape, which is important for how the cell
will grow, reproduce, obtain nutrients, and move. It protects the cell from osmotic lysis, as the
cell moves from one environment to another or transports in nutrients from its surroundings.
Since water can freely move across both the cell membrane and the cell wall, the cell is at risk
for an osmotic imbalance, which could put pressure on the relatively weak plasma membrane.
Studies have actually shown that the internal pressure of a cell is similar to the pressure found
inside a fully inflated car tire. That is a lot of pressure for the plasma membrane to withstand!
The cell wall can keep out certain molecules, such as toxins, particularly for gram negative
bacteria. And lastly, the bacterial cell wall can contribute to the pathogenicity or disease –
causing ability of the cell for certain bacterial pathogens.
Structure of Peptidoglycan
Let us start with peptidoglycan, since it is an ingredient that both bacterial cell walls have in
common. Peptidoglycan is a polysaccharide made of two glucose derivatives, N-
acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), alternated in long chains. The
chains are cross-linked to one another by a tetrapeptide that extends off the NAM sugar unit,
allowing a lattice-like structure to form. The four amino acids that compose the tetrapeptide
are: L-alanine, D-glutamine, L-lysine or meso-diaminopimelic acid (DPA), and D-alanine.
Typically only the L-isomeric form of amino acids are utilized by cells but the use of the mirror
image D-amino acids provides protection from proteases that might compromise the integrity
of the cell wall by attacking the peptidoglycan. The tetrapeptides can be directly cross-
linked to one another, with the D-alanine on one tetrapeptide binding to the L-lysine/ DPA on
another tetrapeptide. In many gram positive bacteria there is a cross-bridge of five amino acids
such as glycine (peptide interbridge) that serves to connect one tetrapeptide to another. In
either case the cross-linking serves to increase the strength of the overall structure, with more
strength derived from complete cross-linking, where every tetrapeptide is bound in some way
to a tetrapeptide on another NAG-NAM chain.
While much is still unknown about peptidoglycan, research in the past ten years suggests that
peptidoglycan is synthesized as a cylinder with a coiled substructure, where each coil is cross-
linked to the coil next to it, creating an even stronger structure overall.
