SCI 250 Week 1 Staining Lab and Quiz

SCI250 Week 1 Chapter 3 Staining Lab Quiz  (6.7 points each)

Check or underline the correct answer

Section: Multiple Choice

1.     Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids?

• Gram stain
• Schaeffer-Fulton stain
• Acid-fast stain
• Lipid stain
• Spore Stain

2.     Which of the following stains is used to classify microorganisms based on their cell wall content?

• Capsular stain
• Gram stain
• Spore stain
• Negative stain
• Methylene blue

3.     In the Gram stain, Gram-positive bacteria stain with a _________ color due to the incorporation of the _________ dye into their cell wall.

• purple, safranin
• purple, crystal violet
• red, safranin
• red, crystal violet

4.     What is the function of the mordant iodine in a Gram staining procedure?

• A mordant causes the crystal violet to run.
• A mordant fixes the bacteria to the slide.
• A mordant keeps dye attached to an object.
• All of the above

5.     What is the order of reagents used in the Gram stain?

• crystal violet, iodine, safranin, alcohol
• alcohol, crystal violet, iodine, safranin
• iodine, crystal violet, safranin, alcohol
• crystal violet, iodine, alcohol, safranin
• crystal violet, safranin, alcohol, iodine

6.     The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by ________

• spore staining.
• scanning electron microscopy.
• Gram staining
• Negative stain

Section: True or False

7.     Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains.

• True
• False

8.     Older, pure cultures of Gram-positive organisms can appear red when Gram stained.

• True
• False

Section: Matching

9.     Use of two or more dyes to differentiate among bacterial species or to distinguish various structures of an organism; for example, the Gram stain.

• Differential stain
• Flagellar stain
• Gram stain
• Negative stain
• Schaeffer-Fulton spore stain
• Simple stain
• Ziehl-Neelsen acid-fast stain
• Cationic dye (also called basic dye)

10.  A technique for observing flagella by coating the surfaces of flagella with a dye or a metal such as silver.

• Differential stain
• Flagellar stain
• Gram stain
• Negative stain
• Schaeffer-Fulton spore stain
• Simple stain
• Ziehl-Neelsen acid-fast stai
• Cationic dye (also called basic dye)

11.  Technique of staining the background around a specimen, leaving the specimen clear and unstained.

• Differential stain
• Flagellar stain
• Gram stain
• Negative stain
• Schaeffer-Fulton spore stain
• Simple stain
• Ziehl-Neelsen acid-fast stain
• Cationic dye (also called basic dye)

12.  A differential stain used to make endospores easier to visualize.

• Differential stain
• Flagellar stain
• Gram stain
• Negative stain
• Schaeffer-Fulton spore stain
• Simple stain
• Ziehl-Neelsen acid-fast stain
• Cationic dye (also called basic dye)

13.  A single dye used to reveal basic cell shapes and arrangements.

• Differential stain
• Flagellar stain
• Gram stain
• Negative stain
• Schaeffer-Fulton spore stain
• Simple stain
• Ziehl-Neelsen acid-fast stain
• Cationic dye (also called basic dye)

14.  A differential stain for organisms that are not decolorized in alcohol, such as the bacteria that cause Hansen's Disease (leprosy) and tuberculosis.

• Differential stain
• Flagellar stain
• Gram stain
• Negative stain
• Schaeffer-Fulton spore stain
• Simple stain
• Ziehl-Neelsen acid-fast stain
• Cationic dye (also called basic dye)

15.  An ionic compound, used for staining bacteria, in which the positive ion imparts the color.

• Differential stain
• Flagellar stain
• Gram stain
• Negative stain
• Schaeffer-Fulton spore stain
• Simple stain
• Ziehl-Neelsen acid-fast stain
• Cationic dye (also called basic dye)