Lab

Create a 2- to 3-page document in Microsoft Word for providing answers to questions in the following:

Exercise4: Purebacterialcolonies

1.   Whenanagarplateis inoculated,whyis theloopsterilizedaftertheinitialinoculumis puton?

2.   Distinguishbetweena purecultureandamixedculture.

3.   Definea bacterialcolony.Listfourcharacteristicsbywhichbacterialcoloniesmaybe distinguished.

4.   Whyshoulda Petridishnotbe leftopenforanyextendedperiod?

5.   Whydoesthestreakingmethod youusedto inoculateyourplatesresultin isolatedcolonies?

Exercise5: Pourplateandstreakingtechniquetoobtainpurecultures

1.   Discusstherelativeconvenienceofpour-andstreak-platetechniquesinculturingclinical specimens.

2.   Howdoyoudecidewhichcoloniesshouldbepickedfrom aplatecultureofamixedflora?

3.   Whyisitnecessarytomakepuresubculturesoforganismsgrownfrom clinicalspecimens?

4.   Whatkindsofclinicalspecimensmayyielda mixedflorainbacterialcultures?

5.   Whenmorethanonecolonytypeappearsinpureculture,whatarethemostlikelysourcesof extraneouscontamination?

Exercise3: Primarymediaforisolationofmicroorganisms

1.Definea differentialmediumanddiscussitspurpose.

2.Definea selectivemediumanddescribeitsuses.

3.WhyisMacConkeyagarselectiveas wellasdifferential?

4.Whyisbloodagarusefulasaprimaryisolationmedium?

5.Whatis themajordifferencebetweenModifiedThayer-Martin(MTM)andchocolateagar?Whenwould youuseMTMratherthanchocolateagar?

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