Genetics assignment due 3 hours

BIO 340 Activity # 5: Hardy-Weinberg and Chi-square analysis (PAGE 1 of 2)

Created by Dr. Susan A. Holechek for BIO 340 (2017)

TASTERS (p2 + 2pq) NON-TASTERS(q2) Allele frequency

# Frequency # Frequency p q

BIO 340 140 61

USA N.A. 0.25 N.A. 0.75

SS (p2) Ss (2pq) Ss (q2) Total

Observed BIO 340 Counts

Observed BIO 340 Frequency 1

Expected Frequency 0.75 1

Expected Counts

(O-E)2/E X2 =

Test paper S = Sodium benzoate:

The following data was obtained from a previous BIO 340 class. Data obtained from reference papers regarding the USA population are also provided and will be used to determine the “Expected” frequency and counts. You will have to analyze the data and provide phenotype, genotype and allele frequencies as well as perform a chi-square analysis. Complete every single empty square (up to two decimals required).

Name (Last, First): _______________________

Test paper T = Phenyilthiocarbamide (PTC)

TASTERS (p2 + 2pq) NON-TASTERS(q2) Allele frequency

# Frequency # Frequency p q

BIO 340 159 40

USA N.A. 0.70 N.A. 0.30

TT (p2) Tt (2pq) tt (q2) Total

Observed BIO 340 Counts

Observed BIO 340 Frequency 1

Expected Frequency 0.30 1

Expected Counts

(O-E)2/E X2 =

BIO 340 Activity # 5: Hardy-Weinberg and Chi-square analysis (PAGE 2 of 2)

Created by Dr. Susan A. Holechek for BIO 340 (2017)

Name (Last, First): _______________________

TASTERS (p2 + 2pq) NON-TASTERS(q2) Allele frequency

# Frequency # Frequency p q

BIO 340 162 38

USA N.A. N.A. 0.45 0.55

UU (p2) Uu (2pq) uu (q2) Total

Observed BIO 340 Counts

Observed BIO 340 Frequency 1

Expected Frequency 1

Expected Counts

(O-E)2/E X2 =

Test paper U = Thiourea:

Use the Chi-square stable, which can be found in the Chi-Square Analysis of Mendel's Data page of your online textbook as well as in the Module IV Chi-square analysis lecture, in order to determine if the null hypothesis (H0) for each of the Test papers is rejected or not. Write down your answer next to each of the compounds.

Sodium benzoate H0=

Phenyilthiocarbamide(PTC) H0=

ThioureaH0=

How do the BIO 340 results compare to those of previously published data from the USA population? Did you expect these results? Explain why.

Mention 3 reasons why most populations are not in Hardy-Weinberg equilibrium:

1)

2)

3)