due 24 hours

Experiment II, Day 8

October 6, 2016

Analysis of Transductants

Analysis of Transductants •  Observe control plate (no phage). Did anything grow? Why or

why not? •  Add 100µl of correct amino acids to M9 plates, be sure to wash

spreader between plates. •  Using a toothpick, patch 48 transductants, as show below. •  Borrow from neighbors if you don’t have enough colonies, but do

not pick the same colony twice!

Tryptophan

Histidine

Rifampicin Tryptophan

Histidine

Methionine

Incubate  plates,  agar  side  up,  at  30°C.  

(Selecting for Arg+/Met+)

(Selecting for RifR/RifS)

(Permissive plate, selecting for Arg+)