due 24 hours

exp2_day4.pdf

Welcome

Experiment II Day 4

September 22, 2016

ARGININE Rifampicin

ARGININE

TRYPTOPHAN TRYPTOPHAN

***Choose colonies from your Trp/Arg plate that the mixture was plated on last week, as well as the 16 colonies you streaked on Monday***

Add 50µl of each amino acid

The order of the plates is important!!!

Experiment II Day 4

•  Be sure to rinse your spreader with water when changing amino acids.

•  Incubate plates at 37°C.

•  Count the number of colonies on each of your plates and record them in the spreadsheet

Lysogens

•  Inoculate 1 colony from your lysogen plate into 5ml of LB

•  Incubate at 30°C

Clean-Up

•  Glass tubes go in the back under “discard tubes here” sign: –  Separate large and small

•  Please throw away any paper towel, trash, parafilm wrappers, etc.

•  BE SURE YOUR PLATES & CULTURES TUBES ARE AT THE CORRECT TEMPS!