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Experiment II Day 3

September 20, 2016

Experiment II Day 3 •  Examine the controls. •  Assess the purity of all the plates. •  Count the number of transconjugants on each of the selective

plates. •  Record your counts on the class spreadsheet. •  Prepare 8 M9 streptomycin plates with trp and arg as before

per group. •  Purify 48 of the His+ conjugational recombinants. •  Note: Pick isolated individual colonies only.

Streak FROM: Tryp/Arg mixed plate

50 µl Tryp + 50 µl Arg

2  M9,  Str  +  Tryp+Arg  Plates  -­‐>  16  streaks  per  person  

Incubate at 37°C

Return your plates to their bin when you are done counting and streaking