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114 RUBRIC Investigation 19: What Factors Affect the Intensity of Color?

General

· Report is due by beginning of next week’ class. Late lab reports will not be accepted.

· One hard copy of the report per group.

· Attach the contribution sheets to the end of the report.

Formatting (points can be deducted if these items are not followed)

· Title page with Investigation name, date, lab section, group members’ names, TA’s name.

· Must be written in third person, past tense, passive voice; no use of first person, and no contractions or slang. Uniform in spacing (double space) and fonts,

· Label each of the sections listed below, bold or underlined.

· Use proper grammar and spelling. Use superscripts and subscripts for chemical formulas and scientific notation. Remember to put a zero in front of the decimal point.

· Include all bibliographic entries of all reference material used.

Introduction (lpt)

· Brief description of the goal in this experiment

· Brief explanation of the spectroscopy technique to support the investigation and the related concepts: light, transmittance, concentration, Beer-Lambert’s law, etc.

· Brief description of the experimental plan used to achieve your goal.

Procedure (2pts)

· Provide a list of reagents/equipment made available in the lab that you used.

· Molarities of solutions must be listed correctly.

· Proper subscripts and physical states are very important.

· Provide a clear description (in a paragraph style) of the procedures used during the course of the experiment; include concentration/volumes of solutions. This should be detailed enough that another group could duplicate your work.

Results (6pts)

· For one dye color, plot the following functions of %T vs. concentration:

· % transmittance (y-axis) vs. concentration (x-axis)

·

image1.wmf

(

)

2

%

T

vs. concentration

·

image2.wmf

T

%

100

vs. concentration

·

image3.wmf

T

%

vs. concentration

·

image4.wmf

÷

ø

ö

ç

è

æ

100

%

log

T

vs. concentration

· Plot and discuss ALL the functions of % T vs. dye concentration that you calculated.

· For each plot, remember to:

· Label x-axis and y-axis (with units)

· Use appropriate software to find the straight-line trends (such as m, y-intercept)

· Include the R2 factor as a statistical measure of the best straight-line fit

· Show an example of the spreadsheet formulas

· Also write the best fit equation for each plot:

image5.wmf

(

)

(

)

b

ion

concentrat

m

T

f

+

=

%

· For example, if the slope is 0.6 and y-intercept is 4.2 for the

image6.wmf

T

%

vs. concentration plot, then the equation is:
image7.wmf

(

)

2

2

.

4

6

.

0

%

+

´

=

ion

concentrat

T

· Show one example of calculation of making diluted solutions from 100% original dye solution

· Use of proper units

· Include and discuss the percent transmittance, %T, (light vs. dye) in terms of wavelength (which wavelength is best for each dye solution? Why?)

Table 1. Percent Transmittance by Wavelength

Dye Color

430 nm

(Blue/Violet light)

470 nm

(Blue light)

565 nm

(Yellow light)

635 nm

(Red light)

Color 1 e.g. Red

Color 2 e.g. Green

Color 3 e.g. Blue

· Include a table of the original data: dye concentration vs. %T

Table 2. Percent Transmittance of the 10 Dilutions of the Red Food Coloring

Concentration

Blank

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

%T

Discussion (3pts)

a. Which graph gives the best fit line, or the straightest line?

1. Does it match the equation of Beer-Lambert’s law?

2. Explain each term (and its units) of the Beer-Lambert’s equation

b. Discuss how wavelength affected the percent transmittance of dyes

c. Discuss possible limitations in the spectroscopy technique used in this investigation

Conclusion (2pt)

· Restate the goal of this experiment

· Summarize the factors that affect the intensity of color and how

· Summarize the straight-line plot that relates the function of %T vs. dye concentration. Describe the usefulness of having a straight-line plot how is this related to Beer-Lambert’s law.

Works Cited (1pt)

Reference the manual and other reputable sources using ACS format.

Beatriz I. Smith 2/24/15

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