Write a report about project management plan
BudgetProjections1YrProject.xlsx.xlsx
1YearProject
| Proposal Budget Worksheet, 1 Year Project | Fringe Benefits Rate Non-Sponsored and Sponsored 07/01/2015-06/30/2016 | |||||||||
| This worksheet assumes that each subawardee will be issued a new contract each year. | Faculty and Classified Staff | 36.30% | ||||||||
| Enter data in year 1 and it will be projected in all future years using the indicated increment. | Hourly | 8.50% | ||||||||
| Data may be entered in any future year and it will be projected to the end. | Full-Time VCU Student | 0.00% | ||||||||
| Complements of Virginia Commonwealth University | ||||||||||
| **Facilities & Administration (F&A) Rates (O/C = On campus; Off/C = Off Campus) | ||||||||||
| 07/01/13 – Until notice: | ||||||||||
| O/C Research | 52.50% | |||||||||
| Increment Rate | 4.00% | Non-Personnel | Off/C Research | 26.00% | ||||||
| Increment Rate | 4.00% | Personnel | O/C Instruction | 40.00% | ||||||
| Fac. & Class. Fringe | 36.30% | Off/C Instruction | 26.00% | |||||||
| Hourly Fringe Rate | 8.50% | O/C Other | 35.00% | |||||||
| **F&A Rate (See Chart) | 52.50% | Off/C Other | 26.00% | |||||||
| Restricted Rates | ||||||||||
| Other Rates: Legacy On Campus Res | 52.00% | |||||||||
| Personnel | Other Rates: Industry Sponsored Clinical Trial | 25.00% | ||||||||
| Faculty & Classified | Other Rates: Sponsor Restricted Other | 20.00% | ||||||||
| Name | % Effort | Annual Salary | Year 1 | Other Rates: Sponsor Restricted Local | 10.00% | |||||
| Dr. Wu (Genentech) | 5% | 100,000 | 16,250 | Other Rates: Sponsor Restricted Training | 8.00% | |||||
| Project manager-Sr Scientist | 25% | 60,000 | 15,000 | Other Rates: Sponsor Restricted | 5.00% | |||||
| Bioinformaticist | 25% | 60,000 | 15,000 | Other Rates: No F&A Allowed by Sponsor | 0.00% | |||||
| Sequencing Technician-Sonya | 50% | 45,000 | 22,500 | |||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| Subtotals | 68,750 | |||||||||
| Summer & Part-Time Faculty | ||||||||||
| Name | % Effort | Annual Salary | Year 1 | |||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| Subtotals | 0 | |||||||||
| Hourly & Postdoctoral | ||||||||||
| Name | Hrs. Worked | Rate Per Hour | Year 1 | |||||||
| Bioinformactist- George | 20 | 50 | 1,000 | |||||||
| 0 | 0 | 0 | ||||||||
| 0 | 0 | 0 | ||||||||
| 0 | 0 | 0 | ||||||||
| 0 | 0 | 0 | ||||||||
| 0 | 0 | 0 | ||||||||
| 0 | 0 | 0 | ||||||||
| 0 | 0 | 0 | ||||||||
| 0 | 0 | 0 | ||||||||
| 0 | 0 | 0 | ||||||||
| Subtotals | 1,000 | |||||||||
| Nonfringe & Students | ||||||||||
| Name | % Effort | Annual Salary | Year 1 | |||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| 0% | 0 | 0 | ||||||||
| Subtotals | 0 | |||||||||
| Subtotal Personnel | 69,750 | |||||||||
| Fringe Faculty & Classified | 24,956 | |||||||||
| Fringe Summer & Part-Time Faculty | 0 | |||||||||
| Fringe Hourly | 85 | |||||||||
| Postdoctoral Health Ins. | 0 | |||||||||
| Subtotal Fringe | 25,041 | |||||||||
| Total Personnel | 94,791 | |||||||||
| Consultants | ||||||||||
| (Names) | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| Subtotals | 0 | |||||||||
| Equipment (>$5,000 each) | ||||||||||
| Servers | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| Subtotals | 0 | |||||||||
| Supplies | ||||||||||
| (Itemize) | ||||||||||
| MasterPure Complete RNA & DNA Purification Kit | 376 | |||||||||
| LB media | 48 | |||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| Subtotals | 424 | |||||||||
| Patient Care | ||||||||||
| Inpatient | 0 | |||||||||
| Outpatient | 0 | |||||||||
| Subtotals | 0 | |||||||||
| Alterations/Renovations | ||||||||||
| (Itemize) | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| Subtotals | 0 | |||||||||
| Travel/Shipping | ||||||||||
| (Itemize) | ||||||||||
| Shipping E.coli from KY Genentech/Roche location | 110 | |||||||||
| 0 | ||||||||||
| 0 | ||||||||||
| Subtotals | 110 | |||||||||
| Other | ||||||||||
| (Itemize) | 0 | |||||||||
| VCU Complete Run Supplies+Service | 10,000 | |||||||||
| VCU Full Gene Expression Analysis Supplies +Service | 25,000 | |||||||||
| VCU Mate Paired Run Supplies + Service | 8,000 | |||||||||
| 0 | ||||||||||
| Total Rent | ||||||||||
| Rent | 0 | 0 | ||||||||
| Total Tuition | ||||||||||
| Tuition | 0 | 0 | ||||||||
| Subtotals | 43,000 | |||||||||
| Subtotal Direct Cost at VCU | 138,325 | |||||||||
| 138,325 | ||||||||||
| Subawards | ||||||||||
| Subawardee #1 | ||||||||||
| Direct | 0 | |||||||||
| Indirect | 0 | |||||||||
| 0 | ||||||||||
| Subawardee #2 | ||||||||||
| Direct | 0 | |||||||||
| Indirect | 0 | |||||||||
| 0 | ||||||||||
| Subawardee #3 | ||||||||||
| Direct | 0 | |||||||||
| Indirect | 0 | |||||||||
| 0 | ||||||||||
| Subawardee #4 | ||||||||||
| Direct | 0 | |||||||||
| Indirect | 0 | |||||||||
| 0 | ||||||||||
| Total Direct Subawards | 0 | |||||||||
| Total Indirect Subawards | 0 | |||||||||
| Total Direct | 138,325 | |||||||||
| F&A Base | 138,325 | |||||||||
| Total F&A | 72,621 | |||||||||
| Total Costs | 210,946 | |||||||||
| 1-year DC total | 138,325 | |||||||||
| 1-year F&A base | 138,325 | |||||||||
| 1-year F&A total | 72,621 | |||||||||
| 1-yr total cost | 210,946 | |||||||||
| For NIH Applications: | ||||||||||
| Total VCU Direct Costs | 138,325 | |||||||||
| Consortium/Contractual Direct Costs | 0 | |||||||||
| Subtotal Direct Costs for Initial Budget Period (Item 7a, Face Page) | 138,325 | |||||||||
| Consortium/Contractual F&A Costs | 0 | |||||||||
| Total Direct Costs for Initial Budget Period | 138,325 | |||||||||
__MACOSX/._BudgetProjections1YrProject.xlsx.xlsx
Management Plan.pptx
High Throughput Sequencing of a Genetically Modified Microbe
Project Management Plan
1
Project Definition
Develop a budget estimate and operating plan for the sequencing of the genome of a genetically modified microorganism, E.coli.
Image Credit: http://s.wsj.net/public/resources/images/OB-ZC073_0930ec_F_20130930040254.jpg
2
Project Summary
Dr. Wu of Genentech
Genetically Modified E. coli
Characterize the complete genome
Characterize the complete transcriptome
Develop a project plan and a cost estimate
Image Credit: http://www.cbc.ca/news/technology/scientists-design-gmos-that-can-t-escape-into-the-wild-1.2929554
3
Bioinformatic Analysis
Image Credit: http://www.tgac.ac.uk/uploads/images/_DSC3572%20copy.jpg
http://www.jscimedcentral.com/Bioinformatics/images/Bioinformatics-banner4.jpg
Measure of Success: High quality analysis
Milestone of Progress: Complete processing of all data
Now it’s a time for some computational power
As a bioinformatics
Wet lab result
Analyze the data by Writing bunch of codes for
arranging the genome sequence, discover the mutation and modify the gene, and find the differences in the genome.
4
Results Assessment & Presentation to Dr. Wu
Image Credit: http://www.chicagonow.com/healthy-human/files/2015/12/97330-93928.jpg
http://dna.cs.byu.edu/bio465/puzzle.png
Measure of Success: Dr. Wu accepts the proposal
Milestone of Progress: Complete review and presentation
Deliverables
Whole Genome Sequence
Distance between Genes
RNA Expression Data
Understanding of the genetically modified microbe
Image Credit: http://www.bioinformatics.babraham.ac.uk/projects/fastqc/fastqc.png
https://seqcore.brcf.med.umich.edu/sites/default/files/images/normal3730.gif
Required Resources
VCU Sequencing Core Facilities
DNA extraction kits
Thermocycler
Pipettes
Nanodrop or Qbit analyzer
Library preparation kit
Sequencing reagents
Sample index kit
Freezer and fridge
Sequencer
Computer for data analysis
Image Credit: http://hihg.med.miami.edu/images/sized/images/_MG_685623newL-460x215.jpg
Required Resources
LB Broth
Erlenmeyer flasks
Test Tubes
Wu’s VCU collaborator’s lab
Bacterial Incubator
Bacterial Incubator/Shaker
Freezer
Fume Hood
Genetically Modified E.coli Sample
Image Credit: http://hihg.med.miami.edu/images/sized/images/_MG_685623newL-460x215.jpg
Good Laboratory Practices
21 CFR Part 58
Standard Sequencing Protocols
Standard DNA & RNA Extraction
Lab Notebooks
Adequate Facility
Lab Coats
Lab Gloves
Separate Rooms for Isolation
Storage of Sequence Data
Image Credit: http://www.esus.ie/projects-elearning/1/GoodLaboratoryPractice.png
Timetable for Tracking
| Task | Start Date | Time (days) |
| E.coli Shipment | 1-Jun | 2 |
| E.coli Growth | 3-Jun | 1 |
| DNA & RNA purification Kit | 4-Jun | 1 |
| Sequencing | 4-Jun | 90 |
| Bioinformatic Analysis | 4-Sep | 90 |
| Results Assessment/Presentation | 22-Sep | 14 |
Cost
| Personnel | Year 1 Cost |
| Dr. Wu (Genentech) | 16,250 |
| Project Manger-Sr. Scientist | 15,000 |
| Bioinformaticist | 15,000 |
| Sequencing Technician | 22,500 |
| Data Manager | 1,000 |
| Fringe Benefits | 25,041 |
| F&A | 93,706 |
| Supplies | Year 1 Cost |
| MasterPure Complete RNA & DNA Purification Kit | 376 |
| LB media | 48 |
| Total | 424 |
| Shipping | Year 1 Cost |
| Shipping E.coli from KY Genentech location | 110 |
| Other | Year 1 Cost |
| VCU Complete Run Supplies+Service | 10,000 |
| VCU Full Gene Expression Analysis Supplies +Service | 25,000 |
| VCU Mate Paired Run Supplies + Service | 8,000 |
| Total | 43,000 |
Total Cost for Project: 210,946
Recap Milestones & Measures
Task 1:
Milestone of progress: Viable E.coli arrived in the VCU lab
Task 2:
Measure of Success: Genetically modified bacteria grow and grow to a healthy concentration (108)
Milestone of Progress: E.coli grows
Task 3:
Measure of Success: The DNA and RNA are pure; the concentration high
Milestone of Progress: Isolation of DNA and RNA
Task 4:
Measure of Success: Get good quality results
Milestone of Progress: Complete the sequencing of the whole genome
Recap Milestones & Measures
Task 5:
Measure of Success: Get good quality results
Milestone of Progress: Complete the sequencing of the mate paired analysis
Task 6:
Measure of Success: Get good quality results
Milestone of Progress: Complete the expression analysis
Task 7:
Measure of Success: High quality analysis
Milestone of Progress: Complete processing of all data
Task 8
Measure of Success: Dr. Wu accepts the proposal
Milestone of Progress: Complete review and presentation
Executive Summary
Mission: Develop a project management plan to characterize a genetically modified E.coli
Objectives:
Obtain Whole Genome Sequence
Expression Analysis of Genome
Distances between Genes
Cost for a year is $209,291
Project Duration: 6 Months
References
__MACOSX/._Management Plan.pptx
Project II High throughput sequencing modified microbe.docx
Project II High throughput sequencing of a genetically modified microbe
The Solexa high though-put sequencer is the one of the fastest gene sequence analyzers in the world. Having successfully inserted some genes into e. coli to create a new mutant microorganism, molecular geneticist Dr. Wu of Genentech wants to characterize the complete genome of the mutant. You have been asked by Dr. Wu to give him a cost estimate for this work. If the following laboratory analyses and bioinformatics data analysis are required, determine the cost for this project for Dr. Wu.
One complete run on the high throughput Solexa sequencing instrument is needed at a cost of $10,000 in service charges from the VCU Genome Sequencing Center. In addition one full gene expression analysis on the Solexa at a cost of $2500 is required, as well as two additional mate paired runs on the Solexa at a cost of $4000 each. The sequencing will take about two weeks effort by a post doctoral scientists paid $45,000 annually. The labor for the laboratory sequencing work is included in the service center cost, but the cost for the bioinformatics data analysis is not. The High Performance Computing Center at VCU houses adequate computing capability, but the cost for the time and effort of a masters degree bioinformatics specialist must be covered by the project.
The data analysis will require determination of genome arrangement, mutation discovery and differentiation. The time estimated for the bioinformatics specialist may vary somewhat, but we estimate that it will take three months. The overhead is covered by the university Facilities and Administration cost, which is 50% on research projects such as this. If the BNFO specialist is paid $60,000 annually and his/her fringe benefits rate is 33.3%, develop a budget for Dr. Wu and justify each item for this laboratory research project.
__MACOSX/._Project II High throughput sequencing modified microbe.docx
Project II Project Management Plan Report Guide.docx
Project II Project Management Plan Report Guide
5. Objectives and deliverables
6. Tasks and timeline
7. Budget work sheet (as an Excel spreadsheet) with spaces for personnel, supplies, materials, equipment, travel, and other (including contracts for major equipment installation or maintenance contracts for equipment). This is the spreadsheet the university likes to use for research proposals. This is for your project management budget
8. Budget narrative
10. References