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Lab scenario​: Several patients have submitted DNA to be tested for a disease marker. Your group will receive three replicate DNA samples from two “patients”*. The results from all BME100 teams’ reactions will eventually be collected on a master chart for statistical analysis. If you mix up or mislabel your samples, you could misdiagnose a “patient.” 1. Make sure that your group has all of the Materials​listed in the protocol you wrote in Lab A. 2. Cut the strip of empty PCR tubes in half so that you have two strips of four linked tubes. This is necessary to fit all of your tubes into the OpenPCR machine. 3. Use a black marker to label the sides of the empty tubes with the Tube Labels​your group created. Do NOT label the lids (tops) of the tubes! 4. Place the tubes in a rack. 5. Start with the empty​tube you labeled as the positive control. Using proper pipetting technique, transfer 50 μL of PCR reaction mix into this empty tube. Discard​the disposable tip into the collection cup. Do not reuse tips and cross contaminate your samples! 6. Using a fresh pipette tip, transfer the positive control DNA/ primer mix into the same tube. The total volume in your positive control PCR reaction tube is now 100 μL. 7. Repeat steps 5 and 6 for the negative control, patient 1 replicates 1, 2, and 3, and patient 2 replicates 1, 2, and 3. Use the appropriate DNA/ primer mix for the corresponding tubes. When you are done, all of your labeled tubes should contain DNA/ primer mix plus PCR mix, resulting in a 100 μL complete PCR reaction​in each tube. 8. Close the lids tightly on your PCR reaction tubes. 9. Take the tubes over to your assigned PCR machine. Place the tubes into the slots in the heating block. Do not start the machine until all 16 slots are filled (multiple groups will need to run reactions simultaneously). If you are unsure whether it is okay to start a run, ask a TA for help.