Water quality report

____________________________________________________________ Report Information from ProQuest December 09 2015 16:41 ____________________________________________________________ COLUMBIA SOUTHERN UNIVERSITY LIBRARY ____________________________________________________________ Table of contents 1. Identifying fecal pollution sources using 3M^sup (TM)^ Petrifilm^sup (TM)^ count plates and antibiotic resistance analysis in the Horse Creek Watershed in Aiken County, SC (USA) ____________________________________________________________ Document 1 of 1 Identifying fecal pollution sources using 3M^sup (TM)^ Petrifilm^sup (TM)^ count plates and antibiotic resistance analysis in the Horse Creek Watershed in Aiken County, SC (USA) Author: Harmon, S Michele; West, Ryan T; Yates, James R http://search.proquest.com/docview/1617002732?accountid=33337 Abstract: Sources of fecal coliform pollution in a small South Carolina (USA) watershed were identified using inexpensive methods and commonly available equipment. Samples from the upper reaches of the watershed were analyzed with 3M^sup (TM)^ Petrifilm^sup (TM)^ count plates. We were able to narrow down the study's focus to one particular tributary, Sand River, that was the major contributor of the coliform pollution (both fecal and total) to a downstream reservoir that is heavily used for recreation purposes. Concentrations of total coliforms ranged from 2,400 to 120,333 cfu/100 mL, with sharp increases in coliform counts observed in samples taken after rain events. Positive correlations between turbidity and fecal coliform counts suggested a relationship between fecal pollution and stormwater runoff. Antibiotic resistance analysis (ARA) compared antibiotic resistance profiles of fecal coliform isolates from the stream to those of a watershed-specific fecal source library (equine, waterfowl, canines, and untreated sewage). Known fecal source isolates and unknown isolates from the stream were exposed to six antibiotics at three concentrations each. Discriminant analysis grouped known isolates with an overall average rate of correct classification (ARCC) of 84.3 %. A total of 401 isolates from the first stream location were classified as equine (45.9 %), sewage (39.4 %), waterfowl (6.2 %), and feline (8.5 %). A similar pattern was observed at the second sampling location, with 42.6 % equine, 45.2 % sewage, 2.8 % waterfowl, 0.6 % canine, and 8.8 % feline. While there were slight weather-dependent differences, the vast majority of the coliform pollution in this stream appeared to be from two sources, equine and sewage. This information will contribute to better land use decisions and further justify implementation of low-impact development practices within this urban watershed.[PUBLICATION ABSTRACT] Subject: Studies; Environmental monitoring; Antibiotics; Drug resistance; Water quality; Water pollution; Pollutants; Feces; Watershed management; Microbiology; Ecology Location: United States--US, Aiken County South Carolina Classification: 9130: Experimental/theoretical; 1540: Pollution control; 9190: United States Title: Identifying fecal pollution sources using 3M^sup (TM)^ Petrifilm^sup (TM)^ count plates and antibiotic resistance analysis in the Horse Creek Watershed in Aiken County, SC (USA) Publication title: Environmental Monitoring and Assessment Volume: 186 Issue: 12 Pages: 8215-8227 Publication year: 2014 Publication date: Dec 2014 Publisher: Springer Science & Business Media Place of publication: Dordrecht Country of publication: Netherlands Publication subject: Environmental Studies ISSN: 0167-6369 Source type: Scholarly Journals Language of publication: English Document type: Feature Document feature: References DOI: http://dx.doi.org/10.1007/s10661-014-3999-8 Accession number: 25139239 ProQuest document ID: 1617002732 Document URL: http://search.proquest.com/docview/1617002732?accountid=33337 Copyright: Springer International Publishing Switzerland 2014 Last updated: 2014-11-05 Database: ABI/INFORM Complete ____________________________________________________________ Contact us at: http://www.proquest.com/go/pqissupportcontact Copyright © 2015 ProQuest LLC. All rights reserved. Terms and Conditions: http://search.proquest.com/info/termsAndConditions