chemistry lab report
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my_friend_workexperiment4_2.docxExperiment 4: Electropolymerized Conducting Polymers.
Introduction:
Conductive polymers (CP) exhibit very useful properties such as flexibility, solubility [1], electrical conductivity, low energy optical transitions, low ionization potential, and high electron affinity.[2] These characterizations make them such effective candidates for many applications such as antistatic and antimagnetic shielding devices[3], microwave attenuation[4], light emitting devices, optical sensors, enzymatic biosensors[5], electronic circuits, and detectors of odors and flavors. The most widely known conducting polymers are polypyrole, polyanaline, and polythiophene. By applying an electrical potential (reversible reaction), these polymers can be reduced. The role of these polymers when they are used as active templates in biosensor applications is the immobilization of dynamic species on the electrode. This will contribute to enhancing the sensitivity and the accuracy of analyte detection. CPs have been used for stabilizing numerous biological species such as enzymes, antibodies, haptens, DNA, and more interestingly the whole cells. [1]
Aim:
The aim of performing this experiment is to create a conducting polypyrrole film which consists of a stabilized enzyme, identify the film and its characteristics, and utilize it as glucose biosensor.
Procedure:
“Refer to Manual for NANO 3101/8302, Electropolymerized Conducting Polymers, Flinders University, p.24-29.”
Results and Discussion:
In the biosensor uses, the deposition of the polymers on the electrode surface can be done by applying an oxidative potential. During this action, the enzymes can be stabilized, and by modifying the deposition time, the amounts of the deposited layer can be recreated. The sensitivity, selectivity, and the accuracy of detection of the biosensors are reliant on the architecture of the polymer, the biological activity of the enzymatic immobilization, and the electropolymerisation circumstances.
In this experiment, the glucose oxidase (enzyme) was immobilized in a conducting polypyrole film on an electrode to find out their appropriateness as a functioning electrode. The performance of the electrode was measured through a Cyclic Voltammogram (CV) of ferricyanide
The geometric area of the electrode was measured by a ruler, and it was found to be 3.14 mm ²which is identical to 0.00314 cm².
The Randles-Sevcik equation is used in the redox reactions
at 25 C °
Where is the peak current, A is the electrode area (cm²), n is the number of electrons involved, C is the concentration of the bulk (mol/ml) for active species, v is the scan rate (V/s), and D is the diffusion coefficient.
n = 1, therefore
, therefore = 0.002756809.
V = 20mV/s = 0.02 V/s, therefore
C = 10 mM = 0.01 mol/L = 0.00001 mol/mL.
can be determined from figure.1
Figure 1: Cyclic Voltammograms (CV) as a function of escalating the scan rate for Platinum Electrode in ferrricyanide solution.
This can be done by evaluating the average of the anodic and cathodic peak current. An example for tabulating the electrode area experimentally will be shown below. At the scan rate of 20mV/s, the average peak current will be equal to
= 0.00001735.
A is the unknown electrode area.
The calculations above were repeated for the different scan rates, the table below summarizes those calculations
|
Scan Rate (V)- mV/S |
Peak Current ( |
Electrode Area (A) - cm² |
|
20 |
0.00001735 |
0.0165 |
|
50 |
0.00002595 |
0.0156 |
|
100 |
0.00003625 |
0.0154 |
|
200 |
0.0000491 |
0.0147 |
Table.1. A summary of the bulk electrode’s calculations
The CV shown in figure 1 for scan rates ranging from 20 to 200 mV/s indicated a formal potential between 210 and 300 mV, with a difference in peak potential of about 0 mV for some and 40 mV for the others. This means that the system is reversible as the peak potential is reliant on the scan rate and the peak currents increase continuously with the scan rate; these results were comparable to the theory, also mathematical confirmation of these results can be seen in Table1. Additionally, as it can be seen from Table1, when increasing the scan rate, the electrode area decreased over time due in large part to the electrolyte which caused erosion of the electrode.
To explain the mechanism of the CV in more detail, the ferricyanide solution is electrolyzed by placing it in contact with the electrode surface. Then, making that surface amply positive and negative, allows the electron to transfer (. The surface is started at a particular voltage with respect to the reference electrode, which is Ag/AgCl. The electrode voltage is increased and decreased at a linear rate, and finally, the voltage is changed back to the original value at the same linear rate. When the surface becomes sufficiently negative and positive, a solution species exchanges electrons with the surface. This results in a measurable current in the electrode circuitry. When the voltage cycle is reversed, which is the case in this experiment, the electron transfer between electrode and chemical species will often be reversed. These features are illustrated in Figure 1 and 2.
Figure 2: CV for Electrode coating with polypyrole
It was observed that the peak current and the potential were much larger in the electrode coated with polypyrole than those without polymers. Moreover, in the CV of the electrode coated with polypyrole, the background current was notably larger due to the polymers overreacting. It can be implied that their areas at the different scan rates provided were larger mainly because of the polypyrole layer thickness protecting the electrode surface from the corrosion as the polypyrole’s resistivity is strong.
Figure.2 Shows the CV of the electrode coated with polypyrole.
The electrode surface area coated with polymer appeared as a smooth dark film with brown spots, which indicates that the electrode is of high quality.
The table below demonstrates , and E° (reversible potential) at different scan rates:
|
Scan Rate (V)- mV/S |
|
|
|
E° |
|
20 |
|
|
0.00003075 |
27 |
|
50 |
|
|
0.0000579 |
29 |
|
100 |
|
|
0.000097 |
51 |
|
200 |
|
|
0.0001675 |
53 |
Table.2. , and E° for the electrode coated with polypyrrole.
Example of calculating E° at 20 mV/s scan rate.
E° = Ep, anodic – Ep, cathodic = 279 – 252 = 27.
The total charge was found to be 6.779 mc.
In the glucose analysis, it was observed that increasing concentrations of the glucose in the buffer solution leads to an increase in the peak current (Figure 3). The calibration curve supports this result by indicating the concentration of the glucose is proportional to the current.
Figure 3. Time Vs current for the glucose addition.
Conclusion:
In closing, the conductive polymer films are fitting in bioseonsor uses due to their electrochemical sensitivity to the presence of chosen ions in a solution. Therefore the development of a glucose biosensor based on conducting polymers immobilized with enzyme has intrigued many scientists around the world. This is because of its capability to detect different biological species in various environments.
Questions:
1- How do the geometric and experimentally determined electrode’s area compare and what other factors may affect the apparent electrode surface area?
The geometric electrode area (0.00314 cm²) was slightly bigger than the experimentally determined electrode’s area, which is because of the reaction’s effects on the electrode. The factors that might have an effect on the appearance of the surface area include the surface roughness of the electrode [6] as well as the concentration of the electrolyte, polymers and enzymes. Other factors might be the enzyme stability, the amounts of voltages applied to the electrode, the purity of the electrode and the electrolyte, the electrode’s resistivity, reaction time, and any unknown containments in the film, or the solutions.
2- Which area did you use for the subsequent calculations and why?
The calculated electrode area at 200 mV/S scan rate was used for the subsequent calculations due to its capability to obtain the desired plot within the given area.
3- Produce a calibration graph of current vs. glucose concentration.
Figure 4: Calibration Curve of Current Vs.Glucose Concentration
4- Determine the concentration of glucose in the sports drinks?
Figure5: Time Vs Current for Sport Drinks
By finding the maximum current for each addition of sports drinks and applying this number to the equation obtained from the calibration curve, the concentration of glucose in sports drinks can be determined.
From figure 3: y= 82.212x-0.1419 (1)
Where y is the maximum current of each addition of sports drinks, x is the concentration of glucose in sports drinks.
Rearranging ( 1),
After adding 1ml,
= 0.001726 mM
The calculations above were repeated for the 10 additions.
The average concentration was found to be 0.001726 mM, which is the concentration of glucose in sports drinks.
5- If a different amount of enzyme were incorporated into the polymer electrode, how would the results differ?
The enzyme behaves as an organic catalyst in the reaction. In one case, when adding higher amounts of enzyme to the polymer, the reaction will be faster which will cause the production of a higher current. On the other hand, when a smaller amount of enzyme is added, the reaction will be slower; therefore the current will be less.
6- If a lower potential were chosen for the TB analysis, what effect would it have on the plot? Would a lower potential be advantageous? Explain why/why not.
If a lower potential was chosen for the Time Based analysis, the current would be less. This is an advantage because it might lead to increasing the sensitivity of the biosensor which would therefore increase the analyte detection.
7- What range of glucose concentrations in this electrode useful over?
From the data found in the experiment, the range of glucose concentrations would be 0.00115 mM to 0.0107 mM. However, from the literature, the range is 2-30mM.
8- Calculate Km and Vmax by performing a Michaelis-Menten analysis.
Where V is the rate of reaction is the concentration of the glucose, Vmax and Km are constants which can be determined from the calibration curve. Due to the difficulties in solving this equation, the values of these constants obtained from this experiment should be comparable to the literature. Therefore, these values were taken from that reading material; Km was tabulated to be 1.5 mM, while Vmax was 1 nm/s. [7]
9- How does this biosensor compare to other you have used or researched?
Glucose biosensors are extremely capable sensors compared to the others since they can detect different things easily. They can be used in clinical diagnostics, environmental monitoring, food freshness, and biochemical and chemical monitoring. The techniques of manufacturing this biosensor vary greatly due the inexpensive, relatively reliable starting materials.
10- If you were able to repeat the experiment several times what variables would change to optimize the biosensor?
The data that was obtained from the experiment was adequate. However, in order to improve the performance of the biosensor, the area of the electrode could be changed, the concentration of the glucose oxidase might be altered, and the film thickness could be adjusted. Also, different concentrations of the polypyrole and the electrolyte would optimize the biosensor.
11- Discuss the factors that limit the sensors dynamic range and relative sensitivity, the concentration range and errors associate with the biosensor. Also discuss the reproducibility of the technique?
All of the factors that can be optimized to produce a better biosensor affect the overall sensitivity of the biosensor.
The factors that limit the sensors dynamic range are dependent on the scan rate, the polymer’s conductivity, thickness, and resistivity. Furthermore, enzyme stability, concentration of the glucose, and electrode surface area can be considered as other elements that limit the biosensors.
Generally, as glucose concentration increases, so does the current. Thus, concentration is a limiting factor.
The errors that may be associated with the biosensor included, but are not limited to: calibration/human error, concentration of the electrolytic solution, and the thickness of the coated polymer.
The reproducibility of the technique can be demonstrated in the calibration curve of the immobilized enzyme, its stability, and the electrode preparation; illustrating homogenous enzyme kinetics that can be easily repeated. This technique is considered to be a very reliable one because the starting materials such as polypyrole and glucose oxdiaze are readily accessible. Also, using the electrochemical analyzer to perform the CV of the electrodes is convenient even with the facilities with limited resources. Therefore, this analytical method can be simply modified for the determination of other biological species.
References:
1. Voelcker, N., Nanotechnology 3 GE, Laboratory Manual 2010, Adelaide, South Australia Flinders University
2. Gerard, M., A. Chaubey, and B.D. Malhotra, Application of conducting polymers to biosensors, in Biosensors and Bioelectronics. 2002. p. 345-359.
3. Bagotsky, V.S., Fundamentals of Electrochemistry Second ed. The Electrochemical Society Series 2006, Hoboken: Wiley & Sons, Inc.
4. Kuhn, H.H., A.D. Child, and W.C. Kimbrell, Toward real applications of conductive polymers, in Synthetic Metals. 1995. p. 2139-2142.
5. Trojanowicz, M., Application of Conducting Polymers in Chemical Analysis, in Microchimica Acta. 2003, Springer Wien. p. 75-91.
6. De Levie, R., The influence of surface roughness of solid electrodes on electrochemical measurements, in Electrochimica Acta. 1965. p. 113-130.
7. Sadik, O.A., et al., Electropolymerized Conducting Polymers as Glucose Sensors, in Journal of Chemical Education. 1999, American Chemical Society. p. 967-null.
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-7.9620000000001E-5 -7.98300000000013E-5 -8.08400000000003E-5 -8.15400000000003E-5 -8.06000000000012E-5 -8.011E-5 -8.15100000000002E-5 -8.15700000000008E-5 -8.08700000000002E-5 -8.10900000000006E-5 -8.14800000000001E-5 -8.20600000000001E-5 -8.15400000000003E-5 -8.06000000000012E-5 -8.22400000000001E-5 -8.298E-5 -8.17600000000012E-5 -8.19100000000001E-5 -8.246E-5 -8.30400000000012E-5 -8.25500000000014E-5 -8.194E-5 -8.35300000000018E-5 -8.37100000000013E-5 -8.298E-5 -8.30700000000012E-5 -8.34000000000003E-5 -8.441E-5 -8.40100000000002E-5 -8.34700000000001E-5 -8.49300000000012E-5 -8.49900000000013E-5 -8.40100000000002E-5 -8.438E-5 -8.49900000000013E-5 -8.59700000000003E-5 -8.49900000000013E-5 -8.45000000000015E-5 -8.59700000000003E-5 -8.636E-5 -8.594E-5 -8.61500000000008E-5 -8.65800000000002E-5 -8.72200000000003E-5 -8.65800000000002E-5 -8.59700000000003E-5 -8.78600000000002E-5 -8.82600000000014E-5 -8.728E-5 -8.76200000000012E-5 -8.82600000000014E-5 -8.89000000000013E-5 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Potential (mV)
Current(A)
Glucose 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5 11.0 11.5 12.0 12.5 13.0 13.5 14.0 14.5 15.0 15.5 16.0 16.5 17.0 17.5 18.0 18.5 19.0 19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.5 25.0 25.5 26.0 26.5 27.0 27.5 28.0 28.5 29.0 29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.5 34.0 34.5 35.0 35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.0 39.5 40.0 40.5 41.0 41.5 42.0 42.5 43.0 43.5 44.0 44.5 45.0 45.5 46.0 46.5 47.0 47.5 48.0 48.5 49.0 49.5 50.0 50.5 51.0 51.5 52.0 52.5 53.0 53.5 54.0 54.5 55.0 55.5 56.0 56.5 57.0 57.5 58.0 58.5 59.0 59.5 60.0 60.5 61.0 61.5 62.0 62.5 63.0 63.5 64.0 64.5 65.0 65.5 66.0 66.5 67.0 67.5 68.0 68.5 69.0 69.5 70.0 70.5 71.0 71.5 72.0 72.5 73.0 73.5 74.0 74.5 75.0 75.5 76.0 76.5 77.0 77.5 78.0 78.5 79.0 79.5 80.0 80.5 81.0 81.5 82.0 82.5 83.0 83.5 84.0 84.5 85.0 85.5 86.0 86.5 87.0 87.5 88.0 88.5 89.0 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 95.5 96.0 96.5 97.0 97.5 98.0 98.5 99.0 99.5 100.0 100.5 101.0 101.5 102.0 102.5 103.0 103.5 104.0 104.5 105.0 105.5 106.0 106.5 107.0 107.5 108.0 108.5 109.0 109.5 110.0 110.5 111.0 111.5 112.0 112.5 113.0 113.5 114.0 114.5 115.0 115.5 116.0 116.5 117.0 117.5 118.0 118.5 119.0 119.5 120.0 120.5 121.0 121.5 122.0 122.5 123.0 123.5 124.0 124.5 125.0 125.5 126.0 126.5 127.0 127.5 128.0 128.5 129.0 129.5 130.0 130.5 131.0 131.5 132.0 132.5 133.0 133.5 134.0 134.5 135.0 135.5 136.0 136.5 137.0 137.5 138.0 138.5 139.0 139.5 140.0 140.5 141.0 141.5 142.0 142.5 143.0 143.5 144.0 144.5 145.0 145.5 146.0 146.5 147.0 147.5 148.0 148.5 149.0 149.5 150.0 150.5 151.0 151.5 152.0 152.5 153.0 153.5 154.0 154.5 155.0 155.5 156.0 156.5 157.0 157.5 158.0 158.5 159.0 159.5 160.0 160.5 161.0 161.5 162.0 162.5 163.0 163.5 164.0 164.5 165.0 165.5 166.0 166.5 167.0 167.5 168.0 168.5 169.0 169.5 170.0 170.5 171.0 171.5 172.0 172.5 173.0 173.5 174.0 174.5 175.0 175.5 176.0 176.5 177.0 177.5 178.0 178.5 179.0 179.5 180.0 180.5 181.0 181.5 182.0 182.5 183.0 183.5 184.0 184.5 185.0 185.5 186.0 186.5 187.0 187.5 188.0 188.5 189.0 189.5 190.0 190.5 191.0 191.5 192.0 192.5 193.0 193.5 194.0 194.5 195.0 195.5 196.0 196.5 197.0 197.5 198.0 198.5 199.0 199.5 200.0 200.5 201.0 201.5 202.0 202.5 203.0 203.5 204.0 204.5 205.0 205.5 206.0 206.5 207.0 207.5 208.0 208.5 209.0 209.5 210.0 210.5 211.0 211.5 212.0 212.5 213.0 213.5 214.0 214.5 215.0 215.5 216.0 216.5 217.0 217.5 218.0 218.5 219.0 219.5 220.0 220.5 221.0 221.5 222.0 222.5 223.0 223.5 224.0 224.5 225.0 225.5 226.0 226.5 227.0 227.5 228.0 228.5 229.0 229.5 230.0 230.5 231.0 231.5 232.0 232.5 233.0 233.5 234.0 234.5 235.0 235.5 236.0 236.5 237.0 237.5 238.0 238.5 239.0 239.5 240.0 240.5 241.0 241.5 242.0 242.5 243.0 243.5 244.0 244.5 245.0 245.5 246.0 246.5 247.0 247.5 248.0 248.5 249.0 249.5 250.0 250.5 251.0 251.5 252.0 252.5 253.0 253.5 254.0 254.5 255.0 255.5 256.0 256.5 257.0 257.5 258.0 258.5 259.0 259.5 260.0 260.5 261.0 261.5 262.0 262.5 263.0 263.5 264.0 264.5 265.0 265.5 266.0 266.5 267.0 267.5 268.0 268.5 269.0 269.5 270.0 270.5 271.0 271.5 272.0 272.5 273.0 273.5 274.0 274.5 275.0 275.5 276.0 276.5 277.0 277.5 278.0 278.5 279.0 279.5 280.0 280.5 281.0 281.5 282.0 282.5 283.0 283.5 284.0 284.5 285.0 285.5 286.0 286.5 287.0 287.5 288.0 288.5 289.0 289.5 290.0 290.5 291.0 291.5 292.0 292.5 293.0 293.5 294.0 294.5 295.0 295.5 296.0 296.5 297.0 297.5 298.0 298.5 299.0 299.5 300.0 300.5 301.0 301.5 302.0 302.5 303.0 303.5 304.0 304.5 305.0 305.5 306.0 306.5 307.0 307.5 308.0 308.5 309.0 309.5 310.0 310.5 311.0 311.5 312.0 312.5 313.0 313.5 314.0 314.5 315.0 315.5 316.0 316.5 317.0 317.5 318.0 318.5 319.0 319.5 320.0 320.5 321.0 321.5 322.0 322.5 323.0 323.5 324.0 324.5 325.0 325.5 326.0 326.5 327.0 327.5 328.0 328.5 329.0 329.5 330.0 330.5 331.0 331.5 332.0 332.5 333.0 333.5 334.0 334.5 335.0 335.5 336.0 336.5 337.0 337.5 338.0 338.5 339.0 339.5 340.0 340.5 341.0 341.5 342.0 342.5 343.0 343.5 344.0 344.5 345.0 345.5 346.0 346.5 347.0 347.5 348.0 348.5 349.0 349.5 350.0 350.5 351.0 351.5 352.0 352.5 353.0 353.5 354.0 354.5 355.0 355.5 356.0 356.5 357.0 357.5 358.0 358.5 359.0 359.5 360.0 360.5 361.0 361.5 362.0 362.5 363.0 363.5 364.0 364.5 365.0 365.5 366.0 366.5 367.0 367.5 368.0 368.5 369.0 369.5 370.0 370.5 371.0 371.5 372.0 372.5 373.0 373.5 374.0 374.5 375.0 375.5 376.0 376.5 377.0 377.5 378.0 378.5 379.0 379.5 380.0 380.5 381.0 381.5 382.0 382.5 383.0 383.5 384.0 384.5 385.0 385.5 386.0 386.5 387.0 387.5 388.0 388.5 389.0 389.5 390.0 390.5 391.0 391.5 392.0 392.5 393.0 393.5 394.0 394.5 395.0 395.5 396.0 396.5 397.0 397.5 398.0 398.5 399.0 399.5 400.0 400.5 401.0 401.5 402.0 402.5 403.0 403.5 404.0 404.5 405.0 405.5 406.0 406.5 407.0 407.5 408.0 408.5 409.0 409.5 410.0 410.5 411.0 411.5 412.0 412.5 413.0 413.5 414.0 414.5 415.0 415.5 416.0 416.5 417.0 417.5 418.0 418.5 419.0 419.5 420.0 420.5 421.0 421.5 422.0 422.5 423.0 423.5 424.0 424.5 425.0 425.5 426.0 426.5 427.0 427.5 428.0 428.5 429.0 429.5 430.0 430.5 431.0 431.5 432.0 432.5 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1.035E-6 1.034E-6 1.031E-6 1.03E-6 1.028E-6 1.026E-6 1.025E-6 1.025E-6 1.025E-6 1.025E-6 1.025E-6 1.025E-6 1.025E-6 1.024E-6 1.021E-6 1.021E-6 1.021E-6 1.021E-6 1.02E-6 1.017E-6 1.016E-6 1.015E-6 1.015E-6 1.015E-6 1.015E-6 1.015E-6 1.015E-6 1.015E-6 1.013E-6 1.011E-6 1.01E-6 1.008E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.005E-6 1.003E-6 1.002E-6 1.001E-6 1.001E-6 1.001E-6 1.001E-6 1.001E-6 1.001E-6 9.98800000000002E-7 9.96100000000002E-7 9.96100000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.95500000000003E-7 9.94000000000002E-7 9.91800000000002E-7 9.90900000000003E-7 9.90600000000003E-7 9.89700000000002E-7 9.88500000000003E-7 1.003E-6 1.025E-6 1.025E-6 1.038E-6 1.704E-6 1.635E-6 1.539E-6 1.483E-6 1.448E-6 1.418E-6 1.394E-6 1.373E-6 1.354E-6 1.337E-6 1.321E-6 1.307E-6 1.295E-6 1.281E-6 1.272E-6 1.26E-6 1.251E-6 1.242E-6 1.236E-6 1.228E-6 1.22E-6 1.213E-6 1.205E-6 1.201E-6 1.194E-6 1.188E-6 1.182E-6 1.177E-6 1.172E-6 1.165E-6 1.162E-6 1.157E-6 1.152E-6 1.147E-6 1.142E-6 1.14E-6 1.135E-6 1.132E-6 1.125E-6 1.123E- 6 1.121E-6 1.117E-6 1.113E-6 1.109E-6 1.104E-6 1.103E-6 1.1E-6 1.096E-6 1.093E-6 1.093E-6 1.086E-6 1.084E-6 1.084E-6 1.08E-6 1.079E-6 1.074E-6 1.074E-6 1.07E-6 1.065E-6 1.064E-6 1.064E-6 1.062E-6 1.06E-6 1.056E-6 1.055E-6 1.054E-6 1.051E-6 1.046E-6 1.045E-6 1.045E-6 1.045E-6 1.042E-6 1.04E-6 1.04E-6 1.036E-6 1.035E-6 1.035E-6 1.031E-6 1.029E-6 1.025E-6 1.025E-6 1.025E-6 1.025E-6 1.025E-6 1.021E-6 1.021E-6 1.02E-6 1.016E-6 1.015E-6 1.015E-6 1.015E-6 1.015E-6 1.011E-6 1.009E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.005E-6 1.002E-6 1.001E-6 1.001E-6 1.001E-6 9.99100000000002E-7 9.96100000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.94600000000002E-7 9.91200000000003E-7 9.90600000000003E-7 9.88500000000003E-7 9.86600000000003E-7 9.86300000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.84800000000002E-7 9.82100000000002E-7 9.81400000000002E-7 9.81400000000002E-7 9.81400000000002E-7 9.81100000000002E-7 9.79900000000002E-7 9.77500000000002E-7 9.76600000000003E-7 9.76600000000003E-7 9.76300000000002E-7 9.76300000000002E-7 9.76300000000002E-7 9.76300000000002E-7 9.76300000000002E-7 9.76000000000002E-7 9.74700000000001E-7 9.72600000000001E-7 9.71400000000002E-7 9.70800000000001E-7 9.69500000000002E-7 9.67700000000002E-7 9.66800000000002E-7 9.66800000000002E-7 9.66800000000002E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66200000000003E-7 9.64400000000001E-7 9.63100000000001E-7 9.62200000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61600000000002E-7 9.61000000000002E-7 9.59500000000002E-7 9.58600000000002E-7 9.57300000000003E-7 9.79600000000002E-7 1.076E-6 1.715E-6 1.659E-6 1.581E-6 1.517E-6 1.47E-6 1.439E-6 1.415E-6 1.393E-6 1.372E-6 1.353E-6 1.334E-6 1.318E-6 1.303E-6 1.291E-6 1.278E-6 1.266E-6 1.256E-6 1.247E-6 1.238E-6 1.229E-6 1.22E-6 1.213E-6 1.204E-6 1.199E-6 1.192E-6 1.183E-6 1.179E-6 1.172E-6 1.164E-6 1.161E-6 1.155E-6 1.151E-6 1.143E-6 1.14E-6 1.134E-6 1.13E-6 1.123E-6 1.122E-6 1.118E-6 1.113E-6 1.107E-6 1.103E-6 1.101E-6 1.096E-6 1.093E-6 1.091E-6 1.084E-6 1.084E-6 1.081E-6 1.079E-6 1.074E-6 1.073E-6 1.067E-6 1.064E-6 1.064E-6 1.06E-6 1.059E-6 1.055E-6 1.054E-6 1.05E-6 1.045E-6 1.045E-6 1.045E-6 1.043E-6 1.04E-6 1.039E-6 1.035E-6 1.035E-6 1.033E-6 1.029E-6 1.025E-6 1.025E-6 1.025E-6 1.025E-6 1.021E-6 1.021E-6 1.018E-6 1.016E-6 1.015E-6 1.015E-6 1.014E-6 1.01E-6 1.007E-6 1.006E-6 1.006E-6 1.006E-6 1.006E-6 1.005E-6 1.001E-6 1.001E-6 1.001E-6 9.98500000000003E-7 9.96100000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.95500000000003E-7 9.91800000000002E-7 9.90300000000003E-7 9.87500000000003E-7 9.86300000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.83000000000002E-7 9.81400000000002E-7 9.81400000000002E-7 9.81100000000002E-7 9.77800000000001E-7 9.76600000000003E-7 9.76300000000002E-7 9.76300000000002E-7 9.76300000000002E-7 9.76000000000002E-7 9.72600000000001E-7 9.70800000000001E-7 9.68300000000002E-7 9.66800000000002E-7 9.66800000000002E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66200000000003E-7 9.63100000000001E-7 9.61900000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61600000000002E-7 9.58900000000002E-7 9.57000000000003E-7 9.57000000000003E-7 9.56700000000003E-7 9.56700000000003E-7 9.56700000000003E-7 9.56100000000002E-7 9.52800000000001E-7 9.51500000000003E-7 9.50300000000002E-7 9.48200000000003E-7 9.47300000000002E-7 9.47300000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.44800000000001E-7 9.42700000000003E-7 9.42400000000002E-7 9.42400000000002E-7 9.41800000000002E-7 9.38400000000003E-7 9.37500000000003E-7 9.37500000000003E-7 9.37200000000003E-7 9.37200000000003E-7 9.37200000000003E-7 9.37200000000003E-7 9.37200000000003E-7 9.37200000000003E-7 9.36900000000003E-7 9.35400000000003E-7 9.33200000000002E-7 9.32600000000003E-7 9.31700000000002E-7 9.29300000000001E-7 9.27700000000001E-7 9.27700000000001E-7 9.27700000000001E-7 9.27400000000002E-7 9.27400000000002E-7 9.27400000000002E-7 1.068E-6 1.233E-6 1.389E-6 1.692E-6 1.629E-6 1.548E-6 1.488E-6 1.45E-6 1.425E-6 1.402E-6 1.379E-6 1.357E-6 1.336E-6 1.318E-6 1.301E-6 1.285E-6 1.273E-6 1.259E-6 1.246E-6 1.237E-6 1.224E-6 1.216E-6 1.205E-6 1.198E-6 1.188E-6 1.18E-6 1.172E-6 1.163E-6 1.159E-6 1.152E-6 1.143E-6 1.14E-6 1.133E-6 1.125E-6 1.122E-6 1.117E-6 1.112E-6 1.104E-6 1.103E-6 1.096E-6 1.093E-6 1.087E-6 1.084E-6 1.081E-6 1.077E-6 1.074E-6 1.068E-6 1.064E-6 1.064E-6 1.06E-6 1.055E-6 1.054E-6 1.047E-6 1.045E-6 1.045E-6 1.041E-6 1.039E-6 1.035E-6 1.034E-6 1.029E-6 1.025E-6 1.025E-6 1.025E-6 1.021E-6 1.018E-6 1.015E-6 1.015E-6 1.013E-6 1.008E-6 1.006E-6 1.006E-6 1.006E-6 1.003E-6 1.001E-6 1.001E-6 9.96100000000002E-7 9.95800000000002E-7 9.95800000000002E-7 9.92100000000002E-7 9.89400000000002E-7 9.86300000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.86000000000003E-7 9.85700000000003E-7 9.81800000000002E-7 9.81400000000002E-7 9.80500000000003E-7 9.76900000000002E-7 9.76600000000003E-7 9.76300000000002E-7 9.76300000000002E-7 9.75300000000002E-7 9.71700000000002E-7 9.69800000000001E-7 9.67100000000002E-7 9.66800000000002E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.66500000000003E-7 9.64400000000001E-7 9.62200000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61900000000002E-7 9.61600000000002E-7 9.61000000000002E-7 9.58600000000002E-7 9.57000000000003E-7 9.57000000000003E-7 9.57000000000003E-7 9.56700000000003E-7 9.56700000000003E-7 9.56700000000003E-7 9.56700000000003E-7 9.56700000000003E-7 9.56700000000003E-7 9.56400000000002E-7 9.55500000000003E-7 9.54300000000002E-7 9.52800000000001E-7 9.52100000000002E-7 9.51800000000001E-7 9.51500000000003E-7 9.51200000000002E-7 9.50600000000003E-7 9.50300000000002E-7 9.49700000000002E-7 9.49100000000002E-7 9.48500000000003E-7 9.48200000000003E-7 9.47900000000003E-7 9.47600000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47300000000002E-7 9.47000000000002E-7 9.47300000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.47000000000002E-7 9.46700000000003E-7 9.45700000000002E-7 9.43900000000002E-7 9.42700000000003E-7 9.42400000000002E-7 9.42400000000002E-7 9.42400000000002E-7 9.42400000000002E-7 9.42400000000002E-7
Time (Seconds)
Current (A)
Calibration Curve of Current Vs.Glucose Concentration
Micro 0.00115000000000002 0.00222000000000003 0.00320000000000003 0.0041 0.00500000000000001 0.0058 0.00656 0.00727000000000007 0.00794000000000007 0.00857 0.00917 0.00973 0.0102 0.0107 0.03 0.05 0.1 0.17 0.24 0.3 0.380000000000003 0.42 0.53 0.53 0.610000000000001 0.690000000000001 0.753000000000005 0.742000000000005Concentration (mM)
Current (µA/cm²)
Time Vs Current for Sport Drinks
Drinks 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5 11.0 11.5 12.0 12.5 13.0 13.5 14.0 14.5 15.0 15.5 16.0 16.5 17.0 17.5 18.0 18.5 19.0 19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.5 25.0 25.5 26.0 26.5 27.0 27.5 28.0 28.5 29.0 29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.5 34.0 34.5 35.0 35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.0 39.5 40.0 40.5 41.0 41.5 42.0 42.5 43.0 43.5 44.0 44.5 45.0 45.5 46.0 46.5 47.0 47.5 48.0 48.5 49.0 49.5 50.0 50.5 51.0 51.5 52.0 52.5 53.0 53.5 54.0 54.5 55.0 55.5 56.0 56.5 57.0 57.5 58.0 58.5 59.0 59.5 60.0 60.5 61.0 61.5 62.0 62.5 63.0 63.5 64.0 64.5 65.0 65.5 66.0 66.5 67.0 67.5 68.0 68.5 69.0 69.5 70.0 70.5 71.0 71.5 72.0 72.5 73.0 73.5 74.0 74.5 75.0 75.5 76.0 76.5 77.0 77.5 78.0 78.5 79.0 79.5 80.0 80.5 81.0 81.5 82.0 82.5 83.0 83.5 84.0 84.5 85.0 85.5 86.0 86.5 87.0 87.5 88.0 88.5 89.0 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 95.5 96.0 96.5 97.0 97.5 98.0 98.5 99.0 99.5 100.0 100.5 101.0 101.5 102.0 102.5 103.0 103.5 104.0 104.5 105.0 105.5 106.0 106.5 107.0 107.5 108.0 108.5 109.0 109.5 110.0 110.5 111.0 111.5 112.0 112.5 113.0 113.5 114.0 114.5 115.0 115.5 116.0 116.5 117.0 117.5 118.0 118.5 119.0 119.5 120.0 120.5 121.0 121.5 122.0 122.5 123.0 123.5 124.0 124.5 125.0 125.5 126.0 126.5 127.0 127.5 128.0 128.5 129.0 129.5 130.0 130.5 131.0 131.5 132.0 132.5 133.0 133.5 134.0 134.5 135.0 135.5 136.0 136.5 137.0 137.5 138.0 138.5 139.0 139.5 140.0 140.5 141.0 141.5 142.0 142.5 143.0 143.5 144.0 144.5 145.0 145.5 146.0 146.5 147.0 147.5 148.0 148.5 149.0 149.5 150.0 150.5 151.0 151.5 152.0 152.5 153.0 153.5 154.0 154.5 155.0 155.5 156.0 156.5 157.0 157.5 158.0 158.5 159.0 159.5 160.0 160.5 161.0 161.5 162.0 162.5 163.0 163.5 164.0 164.5 165.0 165.5 166.0 166.5 167.0 167.5 168.0 168.5 169.0 169.5 170.0 170.5 171.0 171.5 172.0 172.5 173.0 173.5 174.0 174.5 175.0 175.5 176.0 176.5 177.0 177.5 178.0 178.5 179.0 179.5 180.0 180.5 181.0 181.5 182.0 182.5 183.0 183.5 184.0 184.5 185.0 185.5 186.0 186.5 187.0 187.5 188.0 188.5 189.0 189.5 190.0 190.5 191.0 191.5 192.0 192.5 193.0 193.5 194.0 194.5 195.0 195.5 196.0 196.5 197.0 197.5 198.0 198.5 199.0 199.5 200.0 200.5 201.0 201.5 202.0 202.5 203.0 203.5 204.0 204.5 205.0 205.5 206.0 206.5 207.0 207.5 208.0 208.5 209.0 209.5 210.0 210.5 211.0 211.5 212.0 212.5 213.0 213.5 214.0 214.5 215.0 215.5 216.0 216.5 217.0 217.5 218.0 218.5 219.0 219.5 220.0 220.5 221.0 221.5 222.0 222.5 223.0 223.5 224.0 224.5 225.0 225.5 226.0 226.5 227.0 227.5 228.0 228.5 229.0 229.5 230.0 230.5 231.0 231.5 232.0 232.5 233.0 233.5 234.0 234.5 235.0 235.5 236.0 236.5 237.0 237.5 238.0 238.5 239.0 239.5 240.0 240.5 241.0 241.5 242.0 242.5 243.0 243.5 244.0 244.5 245.0 245.5 246.0 246.5 247.0 247.5 248.0 248.5 249.0 249.5 250.0 250.5 251.0 251.5 252.0 252.5 253.0 253.5 254.0 254.5 255.0 255.5 256.0 256.5 257.0 257.5 258.0 258.5 259.0 259.5 260.0 260.5 261.0 261.5 262.0 262.5 263.0 263.5 264.0 264.5 265.0 265.5 266.0 266.5 267.0 267.5 268.0 268.5 269.0 269.5 270.0 270.5 271.0 271.5 272.0 272.5 273.0 273.5 274.0 274.5 275.0 275.5 276.0 276.5 277.0 277.5 278.0 278.5 279.0 279.5 280.0 280.5 281.0 281.5 282.0 282.5 283.0 283.5 284.0 284.5 285.0 285.5 286.0 286.5 287.0 287.5 288.0 288.5 289.0 289.5 290.0 290.5 291.0 291.5 292.0 292.5 293.0 293.5 294.0 294.5 295.0 295.5 296.0 296.5 297.0 297.5 298.0 298.5 299.0 299.5 300.0 300.5 301.0 301.5 302.0 302.5 303.0 303.5 304.0 304.5 305.0 305.5 306.0 306.5 307.0 307.5 308.0 308.5 309.0 309.5 310.0 310.5 311.0 311.5 312.0 312.5 313.0 313.5 314.0 314.5 315.0 315.5 316.0 316.5 317.0 317.5 318.0 318.5 319.0 319.5 320.0 320.5 321.0 321.5 322.0 322.5 323.0 323.5 324.0 324.5 325.0 325.5 326.0 326.5 327.0 327.5 328.0 328.5 329.0 329.5 330.0 330.5 331.0 331.5 332.0 332.5 333.0 333.5 334.0 334.5 335.0 335.5 336.0 336.5 337.0 337.5 338.0 338.5 339.0 339.5 340.0 340.5 341.0 341.5 342.0 342.5 343.0 343.5 344.0 344.5 345.0 345.5 346.0 346.5 347.0 347.5 348.0 348.5 349.0 349.5 350.0 350.5 351.0 351.5 352.0 352.5 353.0 353.5 354.0 354.5 355.0 355.5 356.0 356.5 357.0 357.5 358.0 358.5 359.0 359.5 360.0 360.5 361.0 361.5 362.0 362.5 363.0 363.5 364.0 364.5 365.0 365.5 366.0 366.5 367.0 367.5 368.0 368.5 369.0 369.5 370.0 370.5 371.0 371.5 372.0 372.5 373.0 373.5 374.0 374.5 375.0 375.5 376.0 376.5 377.0 377.5 378.0 378.5 379.0 379.5 380.0 380.5 381.0 381.5 382.0 382.5 383.0 383.5 384.0 384.5 385.0 385.5 386.0 386.5 387.0 387.5 388.0 388.5 389.0 389.5 390.0 390.5 391.0 391.5 392.0 392.5 393.0 393.5 394.0 394.5 395.0 395.5 396.0 396.5 397.0 397.5 398.0 398.5 399.0 399.5 400.0 400.5 401.0 401.5 402.0 402.5 403.0 403.5 404.0 404.5 405.0 405.5 406.0 406.5 407.0 407.5 408.0 408.5 409.0 409.5 410.0 410.5 411.0 411.5 412.0 412.5 413.0 413.5 414.0 414.5 415.0 415.5 416.0 416.5 417.0 417.5 418.0 418.5 419.0 419.5 420.0 420.5 421.0 421.5 422.0 422.5 423.0 423.5 424.0 424.5 425.0 425.5 426.0 426.5 427.0 427.5 428.0 428.5 429.0 429.5 430.0 430.5 431.0 431.5 432.0 432.5 433.0 433.5 434.0 434.5 435.0 435.5 436.0 436.5 437.0 437.5 438.0 438.5 439.0 439.5 440.0 440.5 441.0 441.5 442.0 442.5 443.0 443.5 444.0 444.5 445.0 445.5 446.0 446.5 447.0 447.5 448.0 448.5 449.0 449.5 450.0 450.5 451.0 451.5 452.0 452.5 453.0 453.5 454.0 454.5 455.0 455.5 456.0 456.5 457.0 457.5 458.0 458.5 459.0 459.5 460.0 460.5 461.0 461.5 462.0 462.5 463.0 463.5 464.0 464.5 465.0 465.5 466.0 466.5 467.0 467.5 468.0 468.5 469.0 469.5 470.0 470.5 471.0 471.5 472.0 472.5 473.0 473.5 474.0 474.5 475.0 475.5 476.0 476.5 477.0 477.5 478.0 478.5 479.0 479.5 480.0 480.5 481.0 481.5 482.0 482.5 483.0 483.5 484.0 484.5 485.0 485.5 486.0 486.5 487.0 487.5 488.0 488.5 489.0 489.5 490.0 490.5 491.0 491.5 492.0 492.5 493.0 493.5 494.0 494.5 495.0 495.5 496.0 496.5 497.0 497.5 498.0 498.5 499.0 499.5 500.0 500.5 501.0 501.5 502.0 502.5 503.0 503.5 504.0 504.5 505.0 505.5 506.0 506.5 507.0 507.5 508.0 508.5 509.0 509.5 510.0 510.5 511.0 511.5 512.0 512.5 513.0 513.5 514.0 514.5 515.0 515.5 516.0 516.5 517.0 517.5 518.0 518.5 519.0 519.5 520.0 520.5 521.0 521.5 522.0 522.5 523.0 523.5 524.0 524.5 525.0 525.5 526.0 526.5 527.0 527.5 528.0 528.5 529.0 529.5 530.0 530.5 531.0 531.5 532.0 532.5 533.0 533.5 534.0 534.5 535.0 535.5 536.0 536.5 537.0 537.5 538.0 538.5 539.0 539.5 540.0 540.5 541.0 541.5 542.0 542.5 543.0 543.5 544.0 544.5 545.0 545.5 546.0 546.5 547.0 547.5 548.0 548.5 549.0 549.5 550.0 550.5 551.0 551.5 552.0 552.5 553.0 553.5 554.0 554.5 555.0 555.5 556.0 556.5 557.0 557.5 558.0 558.5 559.0 559.5 560.0 560.5 561.0 561.5 562.0 562.5 563.0 563.5 564.0 564.5 565.0 565.5 566.0 566.5 567.0 567.5 568.0 568.5 569.0 569.5 570.0 570.5 571.0 571.5 572.0 572.5 573.0 573.5 574.0 574.5 575.0 575.5 576.0 576.5 577.0 577.5 578.0 578.5 579.0 579.5 580.0 580.5 581.0 581.5 582.0 582.5 583.0 583.5 584.0 584.5 585.0 585.5 586.0 586.5 587.0 587.5 588.0 588.5 589.0 589.5 590.0 590.5 591.0 591.5 592.0 592.5 593.0 593.5 594.0 594.5 595.0 595.5 596.0 596.5 597.0 597.5 598.0 598.5 599.0 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1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.82500000000003E-7 1.83400000000004E-7 1.83400000000004E-7 1.83700000000003E-7 1.83700000000003E-7 1.83700000000003E-7 1.83400000000004E-7 1.83400000000004E-7 1.83100000000003E-7 1.82800000000004E-7 1.82500000000003E-7 1.82200000000004E-7 1.82200000000004E-7 1.81900000000003E-7 1.81600000000004E-7 1.81600000000004E-7 1.81300000000003E-7 1.81300000000003E-7 1.82500000000003E-7 2.72500000000005E-7 2.55100000000003E-7 2.19400000000004E-7 2.05700000000004E-7 2.01100000000001E-7 1.95600000000004E-7 1.95000000000004E-7 1.92900000000004E-7 1.88900000000004E-7 1.85500000000003E-7 1.85200000000004E-7 1.85200000000004E-7 1.85200000000004E-7 1.85200000000004E-7 1.85200000000004E-7 1.85200000000004E-7 1.85200000000004E-7 1.83700000000003E-7 1.81900000000003E-7 1.81000000000004E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80400000000004E-7 1.80400000000004E-7 1.80400000000004E-7 1.80100000000003E-7 1.80100000000003E-7 1.79700000000003E-7 1.79400000000004E-7 1.79100000000003E-7 1.78800000000003E-7 1.78500000000003E-7 1.78500000000003E-7 1.78200000000003E-7 1.77900000000003E-7 1.77600000000003E-7 1.77300000000003E-7 1.77000000000003E-7 1.77000000000003E-7 1.76700000000003E-7 1.76700000000003E-7 1.76400000000003E-7 1.76400000000003E-7 1.76400000000003E-7 1.76100000000003E-7 1.76100000000003E-7 1.76100000000003E-7 1.76100000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75500000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75500000000003E-7 1.75500000000003E -7 1.75500000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75800000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75800000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000 003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.85500000000003E-7 2.01100000000001E-7 2.13000000000004E-7 2.13900000000003E-7 2.02000000000004E-7 1.9530000 0000004E-7 1.92600000000004E-7 1.85900000000003E-7 1.85200000000004E-7 1.85200000000004E-7 1.83400000000004E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80700000000003E-7 1.80400000000004E-7 1.80400000000004E-7 1.80400000000004E-7 1.80400000000004E-7 1.80400000000004E-7 1.80400000000004E-7 1.80400000000004E-7 1.80700000000003E-7 1.80400000000004E-7 1.80400000000004E-7 1.80400000000004E-7 1.80400000000004E-7 1.80400000000004E-7 1.80100000000003E-7 1.79700000000003E-7 1.79100000000003E-7 1.78500000000003E-7 1.77600000000003E-7 1.77000000000003E-7 1.76400000000003E-7 1.76100000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75800000000003E-7 1.75500000000003E-7 1.758 00000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75200000000003E-7 1.75200000000003E-7 1.74900000000003E-7 1.74600000000003E-7 1.74600000000003E-7 1.74300000000003E-7 1.73600000000003E-7 1.73600000000003E-7 1.72700000000003E-7 1.72400000000003E-7 1.72100000000003E-7 1.71500000000002E-7 1.71200000000003E-7 1.71200000000003E-7 1.70900000000002E-7 1.70900000000002E-7 1.70600000000003E-7 1.70600000000003E-7 1.70600000000003E-7 1.70600000000003E-7 1.70600000000003E-7 1.70600000000003E-7 1.70600000000003E-7 1.70600000000003E-7 1.70600000000003E-7 1.70600000000003E-7 1.70300000000002E-7 1.70300000000002E-7 1.70300000000002E-7 1.70300000000002E-7 1.70000000000003E-7 1.70000000000003E-7 1.70000000000003E-7 1.70000000000003E-7 1.69700000000003E-7 1.69700000000003E-7 1.69400000000004E-7 1.69400000000004E-7 1.69100000000003E-7 1.69100000000003E-7 1.69100000000003E-7 1.68800000000004E-7 1.68800000000004E-7 1.68500000000003E-7 1.68500000000003E-7 1.68500000000003E-7 1.68200000000004E-7 1.68200000000004E-7 1.67800000000004E-7 1.67800000000004E-7 1.67500000000003E-7 1.67500000000003E-7 1.67500000000003E-7 1.67200000000004E-7 1.67200000000004E-7 1.67200000000004E-7 1.66900000000003E-7 1.66600000000004E-7 1.66900000000003E-7 1.66600000000004E-7 1.66600000000004E-7 1.66600000000004E-7 1.66600000000004E-7 1.66300000000003E-7 1.66300000000003E-7 1.66300000000003E-7 1.66300000000003E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.70900000000002E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75800000000003E-7 1.83100000000003E-7 2.24900000000005E-7 2.08400000000004E-7 1.91300000000004E-7 1.85200000000004E-7 1.82200000000004E-7 1.80700000000003E-7 1.80400000000004E-7 1.77300000000003E-7 1.75800000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.75500000000003E-7 1.74000000000003E-7 1.71500000000002E-7 1.70600000000003E-7 1.70300000000002E-7 1.70000000000003E-7 1.69100000000003E-7 1.68200000000004E-7 1.67200000000004E-7 1.66600000000004E-7 1.66300000000003E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.66000000000004E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7 1.65700000000003E-7Time(Seconds)
Current(A)
4-20mVs 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0 21.0 22.0 23.0 24.0 25.0 26.0 27.0 28.0 29.0 30.0 31.0 32.0 33.0 34.0 35.0 36.0 37.0 38.0 39.0 40.0 41.0 42.0 43.0 44.0 45.0 46.0 47.0 48.0 49.0 50.0 51.0 52.0 53.0 54.0 55.0 56.0 57.0 58.0 59.0 60.0 61.0 62.0 63.0 64.0 65.0 66.0 67.0 68.0 69.0 70.0 71.0 72.0 73.0 74.0 75.0 76.0 77.0 78.0 79.0 80.0 81.0 82.0 83.0 84.0 85.0 86.0 87.0 88.0 89.0 90.0 91.0 92.0 93.0 94.0 95.0 96.0 97.0 98.0 99.0 100.0 101.0 102.0 103.0 104.0 105.0 106.0 107.0 108.0 109.0 110.0 111.0 112.0 113.0 114.0 115.0 116.0 117.0 118.0 119.0 120.0 121.0 122.0 123.0 124.0 125.0 126.0 127.0 128.0 129.0 130.0 131.0 132 .0 133.0 134.0 135.0 136.0 137.0 138.0 139.0 140.0 141.0 142.0 143.0 144.0 145.0 146.0 147.0 148.0 149.0 150.0 151.0 152.0 153.0 154.0 155.0 156.0 157.0 158.0 159.0 160.0 161.0 162.0 163.0 164.0 165.0 166.0 167.0 168.0 169.0 170.0 171.0 172.0 173.0 174.0 175.0 176.0 177.0 178.0 179.0 180.0 181.0 182.0 183.0 184.0 185.0 186.0 187.0 188.0 189.0 190.0 191.0 192.0 193.0 194.0 195.0 196.0 197.0 198.0 199.0 200.0 201.0 202.0 203.0 204.0 205.0 206.0 207.0 208.0 209.0 210.0 211.0 212.0 213.0 214.0 215.0 216.0 217.0 218.0 219.0 220.0 221.0 222.0 223.0 224.0 225.0 226.0 227.0 228.0 229.0 230.0 231.0 232.0 233.0 234.0 235.0 236.0 237.0 238.0 239.0 240.0 241.0 242.0 243.0 244.0 245.0 246.0 247.0 248.0 249.0 250.0 251.0 252.0 253.0 254.0 255.0 256.0 257.0 258.0 259.0 260.0 261.0 262.0 263.0 264.0 265.0 266.0 267.0 268.0 269.0 270.0 271.0 272.0 273.0 274.0 275.0 276.0 277.0 278.0 279.0 280.0 281.0 282.0 283.0 284.0 285.0 286.0 287.0 288.0 289.0 290.0 291.0 292.0 293.0 294.0 295.0 296.0 297.0 298.0 299.0 300.0 301.0 302.0 303.0 304.0 305.0 306.0 307.0 308.0 309.0 310.0 311.0 312.0 313.0 314.0 315.0 316.0 317.0 318.0 319.0 320.0 321.0 322.0 323.0 324.0 325.0 326.0 327.0 328.0 329.0 330.0 331.0 332.0 333.0 334.0 335.0 336.0 337.0 338.0 339.0 340.0 341.0 342.0 343.0 344.0 345.0 346.0 347.0 348.0 349.0 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Current (A)
