need matlab experts only

upplement:

Capture.JPG I have a data and an image and I need to do the photon counting histogram for this image. Here it is the FCS trace(or the data ) for the image, we need to know the concentration and the intensity for each part of the image that we would select .. And ,I need the portion with the maximum intensities within the image to be marked , need to add a markers for the positions with max. intensities.Also, you may need to look at this

https://www.cellmigration.org/resource/imaging/ppt-pdf/PCH.pdf

here it is the FCS data :

https://www.dropbox.com/s/fom0mcqbe1p2fi7/647%201st%20measurement_C001.tif?dl=0

Please I need detailed explanation for everything and if you have questions or suggestions please add them and if you can add references for me to study for more understanding that would be awesome.

have an FCS trace for an image and need to know the Photon counting Histogram and the concentration  for it in addition to that I need the program to be flexible so I can get the PCH for any part of the image I select ,and if you can do it in matlab or python that would be ok.