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Values of effective height equivalent to a theoretical plate (H) vs. average linear velocity (u) for HARPO (k' = 2.66) at 37 oC on a 10.0-m long Altech Summer Time column with 0.10-mm bore diameter, and packed with a 3 um cyclodextrin bonded stationary phase attached to 3 μm-diameter particles are:

u(cm/s)*: 7.0 12.0 19.0 27.0 45.0

H (mm)**: 0.130 0.100 0.100 0.130 0.170

For each data point calculate tr' and the effective plate numbers.Graph H vs. u and determine uopt and Hmin from the fit of the experimental data to the Van Deemeter expression. Also, report the recovered A, B, C terms with appropriate uncertainties (i.e., +).

* Uncertainty is 5% RSD; **Uncertainty is 7% RSD