writing
Angela Poole
BIO102
2-1-15
Lab report
Measuring Osmosis
Introduction:
The first important in this lab to know the concept of osmosis. Also, in this lab will force in Osmosis because Osmosis part of kinetic energy. Osmosis is a special type of diffusion of movement of molecules from areas of higher concentration to areas of lower concentration or vice versa. However, the best example of osmosis is the diffusion of water goes over membrane. Moreover, water will moved from high concentration to low concentration. The osmosis occurs until dynamic equilibrium has been reached, this is the point where the concentration in both areas equal and they will not movement. We should know the solution.
Hypothesis:
I will try in this experiment to confirm that osmosis occurs from high concentration to low and diffuse down. “is the spontaneous net movement of solvent molecules through a partially permeable membrane into a region of higher solute concentration, in the direction that tends to equalize the solute concentrations on the two sides. It may also be used to describe a physical process in which any solvent moves across a semipermeable membrane (permeable to the solvent, but not the solute) separating two solutions of different concentrations. Osmosis can be made to do work.”
Method:
In this experiment I should have beaker w\disti ited (H2O), CLEP, STRING DIALYSIS BAG, RING STAND, RUBEER SOPPER AND CLAMP. After that I should cut the dialysis bag and secure it with a tie at the end of a soaked tube. First of all to fill the dialysis bag with 20 ml solution containing sucrose. The best things the solution could not cross the membrane. Moreover, I should to tie the end of the dialysis bag using a string to be sure that dialysis bag is completely enveloped with no air gap from the tube. Also it is enveloped to make sure that there are no leaks in the membrane. I should be sure that the outside tune had been washed of any spilled solution. After that I have to record the time every 5 minutes. In addition I should record the data every minutes interval over the next 60 minutes.
Results:
In each group was use different sucrose solution. I was for 20% the result was as follows.
The graph shows the measurements of the 10% sucrose,20% sucrose ,and 40%. Like what I see in the table the 20% was going very slow, but the 10% was going very fast and also the 40% was going very fast that what I saw in the lap last week.
Discussion:
When I did this experiment there were three groups. In my group we used sucrose of 20%, but the other two groups used 10% and 40% of sucrose. Every group checked every five minutes.Observed passive transport for osmosis. I was wrong for my hypothesis that I thought the 20% going fast, but the 10% and 40% are going up every five minutes. I can understand water moves toward the most concentrated solution.” Water potential quantifies the tendency of water to move from one area to another due to osmosis, gravity, mechanical pressure, or matrix effects such as capillary action.” osmosis continues to enter the water through the membrane until equal water.
References:
http://en.wikipedia.org/wiki/Osmosis
http://en.wikipedia.org/wiki/Water_potential
10% Sucrose 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 0.3 0.6 1.3 1.7 2.3 3.0 3.5 4.1 4.6 5.0 5.6 5.9 20% Sucrose 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 1.5 3.6 5.0 5.7 6.5 7.0 8.2 8.8 9.1 9.5 9.7 40% Sucrose 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 0.5 2.0 3.0 5.5 8.5 12.5 15.0 17.5 20.5 24.0 27.0