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Experiment Genetic Inheritance

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Genetic Inheritance Margaret E. Vorndam, M.S. Version 42-0061-00-01

Lab Report Assistant

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Data Table 1: Punnett Square for F1 Cross – Expected Genetic Outcomes

1 Parent, genes:

(student to fill in the blanks)

alleles > alleles v

F 1 Parent,

genes: (student fill in blank)

Data Table 2: Results of F1 Cross

Observed Phenotypes of F 2

Progeny # green plants = # white plants = Total # plants

Petri Dish 1 >

Petri Dish 2 >

Total

Experiment Genetic Inheritance

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Data Table 3: Dihybrid Cross in Corn – Results of P Cross

P = purple, p = yellow S = smooth, s = wrinkled (student to fill in all blanks )

Generation Expected Alleles Expected Alleles

P > dominant x recessive

ppss

F 1 Progeny >

Frequency >

Data Table 3A: Punnett Square for F1 Dihybrid Cross

Expected Genotypic Outcomes (student to fill in)

Parent 1 F 1

– can produce these gametes: (student to fill in)

Parent 2 F 1

– can produce these gametes: >

Shaded portion above represents the F2 progeny genotype and phenotype. Student to fill in.

Experiment Genetic Inheritance

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Data Table 4: Dihybrid Cross in Corn – Results of F1 Cross in F2 Progeny

Phenotype of Progeny

(What they look like – word description)

Genetic Designations possible for this Phenotype

e.g., PPSS

Predicted Allelic Frequency

(Expected Ratio)

Number of Phenotype Counted:

(Observed Number out of 100 counted)

Actual Allelic Frequency

(Observed Ratio)*

Ex: Yellow, wrinkled

ppss, ppss

1÷16= 0.06

4÷100= 0.04

* Actual Allelic Frequency (Observed Ratio) = Number of Phenotype Counted ÷ 100 total kernels counted

Data Table 5: Χ2 Goodness of Fit Test for F Phenotypic Results from F Corn Cross 2 1

Phenotype Description of F

2 Progeny

from Table 4

Observed Number from Table 4

Observed Ratio from Table 4

Expected Ratio from Table 4

* Expected Number, calculated

** [Observed No. – Exp. No.]2 ÷ Expected No.

e.g., Yellow, wrinkled

Σ Sum of column =

c2, Chi-square value *** >

* Expected Number, calculated = Σ Sum of Observed Number x Expected Ratio for that phenotype

** = (Observed number – Expected number, calculated) square ÷ Expected Number, calculated

*** c2, Chi-square value = Σ Sum of (Observed number – Expected number, calculated) squared ÷ Expected Number, calculated

Experiment Genetic Inheritance

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Data Table 6: Summarization of c2 Good Fit Results for F Corn Cross

c2 value from Table 5 =

Value at 3 Degrees of Freedom that is closest to c2 value =

What is the Fit Probability at the top of the column in which the value was found?

What is the % of probability that the observed results match the expected results? (Multiply Fit Probability by 100)

Reading at the top of the Table, this Fit Probability indicates that the expected results hypothesis is a

Good Fit Poor Fit

Circle the correct choice above

Experiment Genetic Inheritance

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Exercise 1: F1 Hybrid Cross

A. Expected phenotypic ratio of green to white progeny: Calculation of Expected Ratio (Frequency) = = Total Number of (Color) Seedlings ÷ Total of All Seedlings.

B. If 320 F 2

offspring resulted from this F 1

cross, how many would be green?

White?

Discussion

A. Did the results support or refute the hypothesis? Explain.

B. How similar are the observed to the expected results from the Punnett square?

C. If the results are not similar, how might the difference be explained?

D. Will a monohybrid F 1

cross in corn yield the same ratio of expected phenotype in progeny as for the tobacco seedlings? Why or why not?

E. If available, compare your F 2

seedling data to those of your classmates. Are the outcome ratios the same? Why might using a larger number of seedlings to determine this outcome be wise?

Experiment Genetic Inheritance

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Exercise 2: Dihybrid Genetic Crosses Procedure

1. Based on what you can conclude about its genetic makeup when told that the corn plant parent cross (P) pictured in Figure 2 is between a completely dominant plant and a completely recessive plant,

a. Construct and record a hypothesis about what the genetic makeup and the frequencies of the alleles for the F

1 progeny plants in the dihybrid cross of corn will be. Record your

hypothesis here:

b. If these F 1

progeny are mated, what will be the resulting allelic frequency for the F 2

progeny? Record this hypothesis here:

Results

A. What are the two hypotheses that you made about the allelic frequencies of progeny produced by the crosses:

P x P?

F 1

x F 1 ?

Experiment Genetic Inheritance

B. Based on what you know about phenotypes and Figure 2, for the P generation, what is the corn plant genotype on each cob containing the P corn kernels? One is completely dominant, so its genotype is

One is completely recessive, so its genotype is .

C. Would it make a difference in the outcome of this cross if the genotype of one parent is PPss and the other is ppSS?

D. From the phenotype of the kernels on each P generation cob what would the predicted genotype of any F

1 plant be?

E. Given the 2n equation predict how many different genetic outcomes will be possible from an F

1 cross resulting in the F

2 generation in a dihybrid corn cross.

Experiment Genetic Inheritance

F. If a F 2

corn cob resulting from this F 1

cross contained 563 seeds, how many of the seeds would you expect to look like the F

1 parent?

Questions

A. How well do the predicted results match the actual results in Table 4?

B. Based on the Punnett Square predictions, can a statement be made as to whether your hypotheses are supported or rejected? Which and why?

C. Dihybrid F

1 crosses result in a predictable F

2 progeny phenotypic frequency that holds true

universally. Based on the Expected outcome, what is it?

D. If your results are not as expected why might there be differences?

E. What applications might this type of genetic investigation have? How might the information be applied medically?

Experiment Genetic Inheritance

Exercise 3: Chi-square and Hypothesis Testing Questions

A. What can be concluded about your prediction of expected F 2

progeny phenotypic outcome from the F

1 cross? Was it close to the observed outcome?

B. How might the c2 test for fitness be used in other ways? Try it on the tobacco seedling F cross, for instance.

C. In a typical cross where a parent with a completely dominant trait is mated with a parent exhibiting a completely recessive trait, what is the expected genotypic outcome and allelic frequency for the F

1 progeny?

Give an example.

D. In a typical cross where hybrid F

1 parents are mated, what is the expected genotypic outcome

and allelic frequency of the F 2

progeny?

Give an example.

Experiment Genetic Inheritance

E. For the F 2

progeny produced from a typical F 1

hybrid mating, how many totally recessive individuals would be produced if the progeny total population is six offspring?

What if the progeny population was 20?

50?

1,000?

F. Excluding factors such as sex-linked genes, incomplete dominance or epistasis, etc., will the above cross results vary if different organisms are used, such as dogs or tulips? Why or why not?

G. How will factors such as sex-linked genes, incomplete dominance, or epistasis, etc., affect the expected outcomes that were investigated above?