Bioenergetics simple questions

Questions (exercise 2):

a) Compare the shapes of the curves for the rate of reaction against L-isocitrate concentration in the presence and absence of ADP. Explain the effect of ADP and the underlying mechanism.

(4 marks)

b) Explain the metabolic significance of the effect of ADP on the activity of isocitrate dehydrogenase.

(2 marks)

c) Name any other intracellular molecules that normally regulate the activity of this enzyme and state whether they are activators or inhibitors and through which mechanism.

(2 marks)

Exercise 3

The data in the table below represents part of the laboratory analysis for a five day old female child rushed into hospital unconscious.

Table 2.Laboratory analysis.

Questions (exercise 3):

d) What condition is the child suffering from? Explain your ‘diagnosis’ in terms of each of the data in the table.

(5 marks)

i) What is the underlying [biochemical] cause of the condition the patient is suffering from? Include details of any relevant enzymatic reactions/pathways.

(3 marks)

j) What ‘unusual’ metabolite, not listed above, would you also expect to be found in the urine of the child? Explain how this metabolite might be related to the diagnosed disorder.

(3 marks)

k) Once stabilised the child was maintained on dietary therapy. Outline what form the dietary therapy would take, including any ‘supplements’ that may be added to the basic diet as required. [please note the patients age]

(4 marks)