ENZYMES
JRAE79
lab_report_-_wk_10_enzymes_1.docx
Enzymes: Temperature, pH, and Specificity
Hands-on labs, inc. Version 42-0054-00-01
LAB REPORT
PHOTOS – Include THREE digital photos with your lab report, either as separate attachments
to an e-mail or paste into your document.
1. Photo #1 – Ex. #1 – Take a photo of the five glucose strips after dipping,
label them a,b,c,d,e to match the data table #1.
2. Photo #2 – Ex. #2 – Take a photo of the 12 glucose strips after dipping,
label them a,b,c, and the times 5,10,15,20 min. to match the data table #2.
3. Photo #3 – Ex. #3 – Take a photo of the four glucose strips after dipping,
label them a,b,c,d, to match the data table #3.
Exercise 1: Enzyme Specificity
Observations
|
Data Table 1: Glucose Concentration |
|
|
Wells |
Concentration of Glucose |
|
a |
|
|
b |
|
|
c |
|
|
d |
|
|
e |
|
Experiment
EnzymEs TEmpEraTurE pH spEcificiTy
142
©Hands-On Labs, Inc.
www.LabPaq.com
Questions
A. What determines a person’s ability to digest lactose?
B. Which of the wells showed a positive result for glucose? Explain the results.
C. Explain why testing for glucose is used to determine the activity of the enzyme lactase.
D. Explain the experimental conditions for the five different wells.
Exercise 2: Enzymes and Temperature
Observations
|
Data Table 2: Presence of glucose in wells indicating lactase activity at various temperatures |
||
|
Well |
Time (min) |
Concentration of Glucose |
|
a |
5 |
|
|
|
10 |
|
|
|
15 |
|
|
|
20 |
|
|
b |
5 |
|
|
|
10 |
|
|
|
15 |
|
|
|
20 |
|
|
c |
5 |
|
|
|
10 |
|
|
|
15 |
|
|
|
20 |
|
Questions
A. What happens when an enzyme is boiled? Is this effect reversible?
C. Based on your experiment results, what is the optimal temperature for lactase function?
D. Explain what happens as far as the effectiveness of the enzyme at the freezing temperature. Can this effect be overcome when the temperature rises?
Exercise 3: Enzymes and pH
Observations
|
Data Table 3: Glucose in wells a-d indicates enzyme activity at various pH levels |
||
|
Well |
pH |
Concentration of Glucose |
|
a |
3.5 |
|
|
b |
5.0 |
|
|
c |
6.8 |
|
|
d |
11.0 |
|
Questions
A. Graph the data placing glucose concentrations on the y-axis and the pH values on the x-axis.
B. What was the effect of pH on the enzyme lactase? Is this true for all enzymes?
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42
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0054
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01
LAB REPORT
PHOTOS
–
Include
THREE
digital photos with your lab report, either as separate attachments
to an e
-
mail or paste into your document.
1.
Photo #1
–
Ex. #1
–
Take a photo of the five glucose strips after dipping,
label them a,b,c,d,e to match the data table #1.
2.
Photo #2
–
Ex. #2
–
Take a photo of the 12 glucose strips after dipping,
label them a,b,c, and the times 5,10,15,20 min. to match the data table #2.
3.
Photo #
3
–
Ex. #3
–
Take a photo of the four glucose strips after dipping,
label them a,b,c,d, to match the data
table #3.
E
x
e
r
cise 1: En
z
yme Specificity
O
bservations
D
a
t
a
T
able 1: Glu
c
ose Conce
n
t
r
a
tion
W
ells
Conce
n
t
r
a
tion
of Glu
c
ose
a
b
c
d
e
Experiment
EnzymEs TEmpEraTurE pH spEcificiTy
14
2
©Hands-On Labs, Inc.
www.LabPaq.com
Enzymes: Temperature, pH, and Specificity
Hands-on labs, inc. Version 42-0054-00-01
LAB REPORT
PHOTOS – Include THREE digital photos with your lab report, either as separate attachments
to an e-mail or paste into your document.
1. Photo #1 – Ex. #1 – Take a photo of the five glucose strips after dipping,
label them a,b,c,d,e to match the data table #1.
2. Photo #2 – Ex. #2 – Take a photo of the 12 glucose strips after dipping,
label them a,b,c, and the times 5,10,15,20 min. to match the data table #2.
3. Photo #3 – Ex. #3 – Take a photo of the four glucose strips after dipping,
label them a,b,c,d, to match the data table #3.
Exercise 1: Enzyme Specificity
Observations
Data Table 1: Glucose Concentration
Wells Concentration of Glucose
a
b
c
d
e
