Cell Membrane Transport

profilefatalbert619
bk-1_cell_membrane-transport_rptv_fix_fall_2013.rtf

Lab Report Name: ____________________

Section: ___________________

1

Cell Membrane Transport

Exercise 1 - Diffusion through an Artificial Membrane

Data Table 1- Changes during Dialysis

Color

Before Dialysis

After Dialysis

Solution in Plastic Cup

Solution in Dialysis Bag

Data Table 2- Benedict’s test Results

Color of Solution

Before Heating

After Heating

IKI solution in 16 oz cup

Solution in Dialysis Bag

Distilled water

Questions:

  • What is the purpose of this exercise? What is being tested?

  • What color change was observed in the dialysis tubing? What does that change indicate?

  • Was there a color change in the water surrounding the tubing? If so, explain.

  • What color change was observed in the water from the 16 oz cup containing the IKI used after heating? What does that change indicate?

  • What does the Benedict’s reagent detect?

  • What does the IKI solution detect?

  • In what way is a cell membrane similar to the model cell made of dialysis tubing?

  • Is the transport mechanism in the model cell passive or active, and why?

Exercise 2 –

Data Table 3: Diffusion of KMnO 4 at Various Temperatures

Questions:

  • How does temperature affect the rate of diffusion?

  • State a general hypothesis to cover how temperature affects rate of diffusion.

Exercise 3 - Tonicity and Diffusion:

Data Table 4-Potato Dimensions

Potato

Before Osmosis

(L x W)cm

After Osmosis

(L x W) cm

Tonicity: Hypertonic, Isotonic or Hypotonic

Distilled water

10% Sodium Chloride

Questions:

  • What is the condition of each potato strip after soaking in the test tubes for an hour? Which one is limp and which one is crisp?

  • How would you explain the difference in the conditions of the potato strips using the concept of tonicity?

  • What was the tonicity of the fresh water solution with respect to the potato cells?

  • What was the tonicity of the salt water solution with respect to the potato cells?

  • How do the changes in the conditions of the potato strips relate to the wilting of plants?

  • How does keeping vegetables cool slow them from wilting?