intro for paper

Master project report

Title (I suggest 2 title to my project u can help me to choose the best one suitable to my project :

1. Brassica lectins act similarly to antibodies by providing a protective cell barrier against pathogenic bacteria and nematodes

2. The role of two Arabidopsis lectins during interaction with pathogenic and soil bacteria

Abstract: this part must be 250 words only no less no more please

I write this small part, I try to write short abstract to help explain the idea of my project to you, so u can start this part.

Also I provide u the aims of this project .which could be good to explain more the idea.

Induced defence gene expression is an effective way for plants to allocate resources against pests and pathogens when needed. Plant lectins are proteins that bind to carbohydrates with high specificity. They have the ability to recognise and bind glycoconjugates on the surface of microorganisms and to agglutinate cells. In this study, two highly inducible members of a lectin-encoding gene family from Arabidopsis thaliana and one from canola were used as potent defence genes in transgenic Arabidopsis and tobacco plants. DNA expression using quantitative-real time PCR and protein-ligand binding assays using glycan microarrays revealed distinctive lectin gene expression patterns during plant defence responses and global carbohydrate binding profiles. These lectins possess a signal peptide and occur in two isoforms (glycosilated and unglycosilated). Lectin-1 binds to mono- and di- saccharides and accumulates in the plant cell wall forming a protective barrier against bacterial invasions by causing effective immobilisation (clumping) similarly to antibodies. Arabidopsis and tobacco plants overexpressing Lectin-1 show strong resistance to Pseudomonas syringae pv tomato and these plants also displayed resistance to root-knot nematode infection.

Aims:

Aims 1: Trаnsform Arаbidopsis plаnts with Lectin 2 gene

In this aim, the transfer of the Arabidopsis plant with the Lectin 2 gene will be performed.

This can be achieved by the germinating and sporting of the wild seeds for about 6 weeks

Then start the transformation with Lectin 2. Then the floral dip methods will be initiated, and

After that all the seeds will be collected to sprout them for the next step

.

Aims 4: Evaluate resistance of Lectin 2 plants аgаinst Pseudomonаs syringаe

In the project we wish to test LECTIN2 for the ability to provide resistance. First LECTIN2- overexpressing plants will be generated. Primary transgenic will be assessed for resistance against P. syringae and possibly other pathogenic bacteria.

Introduction: this part must be 1000 only: ( I need this one now)

In this part I want u to write several paragraphs:

First one: about Arabidopsis plant, why it's to work on?? Idea about it?etc information show the reader what I it

Second about: plant gene transformation and transgenic in plant

What its? Why we use it ? Explain about it

Third: beneficial microbe in soil or plant interaction with soil microbes

Fourth: about Brassica lectins family (Lectin 2 and Canola gene)

Methods: I will send to u the details latter to write it after u finish the part above..