lab report assistant

Macromolecules of Life

Hands-On Labs, Inc. Version 42-0085-00-01

Lab RepoRt assistant

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Exercise 1: Testing for Proteins

Data Table 1: Biuret results.
Substance Tested	Predicted Results	Biuret Color & Number of drops added
1: Egg white
2: Pepsin
3: Sugar
4: Distilled water

Questions

A. Which of the test tubes are the positive and negative controls? B. What conclusions can be made about this experiment?

C. How might biuret reagent be used in a practical real-life situation? D. How did the predicted results compare to the actual results?

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Exercise 2: Testing for Sugars

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Data Table 2: Benedict’s Reagent Results.
Test Sample	Predicted Results	Benedict’s results
#1 Glucose
#2 Starch
# 3 Onion juice
#4 Distilled Water

Questions

A. Which of the test tubes are used as the positive and negative controls? B. How did the predicted results compare to the actual results?

C. What can be concluded from this experiment?

D. How might Benedict’s reagent test be used in a practical real-life situation?

Exercise 3: Testing for Starch

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A. Write a hypothesis about whether a potato or onion has the most starch.

Observations Table: Exercise 3

A. View of potato under pocket microscope. Magnification =

B. View of potato cells with IKI. Magnification =

Observations Table: Exercise 3

A. View of onion cells without IKI. Magnification =

B. View of onion cells with IKI. Magnification =

Questions

A. Which vegetable has the most starch?

B. What is the purpose of starch in vegetables? Hint: What is the function of stored sugar?

C. How might the IKI reagent test be used in a practical real-life situation?

D. Did the results differ from your hypothesis? Explain.

Exercise 4: Testing for Lipids

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Data Table 3: Lipid test results.
Macromolecule Being Tested	Hypothesis: contain lipid or not	Results from test
Potato
Onion
Oil
dH2O

Questions

A. What is the test substance?

B. Which test represents the control?

C. Which test contained the most test substance?

D. Did the results agree with the initial hypothesis in every case? Explain why or not.

Exercise 5: Making Mayonnaise

ConCLusions

A. If you were given a piece of hot dog and a piece of carrot, using what you learned in these exercises, how would you analyze the composition of these materials?

B. What classes of compounds are present in living organisms that are not tested for in this exercise?