WK-2-AP-PT2

BIOS251 OL, Week 2 Lab

Name:

OL Lab 2: Protein Denaturation

Learning Objectives:

· Understand how protein denaturation.

· Understand how interactions between side groups influence the protein structure

· Understand the chemical and physical causes of protein denaturation

· Understand the results of protein denaturation

Introduction:

Proteins are one of the major biomolecules in the body. It has to maintain its proper shape and structure for it to be functional biomolecule. Protein’s structure can be disrupted due to many factors. In this simulation you will explore how chemical and physical factors cause protein denaturation. You will perform various experiments using ovalbumin, the primary protein found in eggs, in order to understand the chemical and physical causes of protein denaturation. Will you be able to unravel the factors that affect a protein molecule?

Part 1: Complete Labster Protein Denaturation

Part 2: Report and Reflection

Purpose: Describe in your own words and in complete sentences, the purpose of this experiment.

Observations: List 3 observations you have made in this simulation.

Answer all the questions below:

1. List out four abundant elements found in the body and state their contribution to body functions. ( 2 points)

2. How does the protein shape determine its function? (1 point)

3. What are enzymes and how does pH and temperature affect enzymatic activity? (3 points)

4. List 3 enzymes that function at different pH in our body.( 3 points)

5. Reflection: Reflect on at least 2 key concepts you have learned from this simulation. How would you relate it to the physiological functions of the body?

Grading Rubric: 

Activity	Deliverable	Points
Part 1	Complete simulation	15
Part 2	Complete lab report and answer questions  · Purpose (1 point)  · Observation (3 points) · Questions (9 points)  · Reflection (2 points)	15
Total	Complete all lab activities	30