measure gel electrophoresis using excel (2 hours)

profilerojv0q

First watch this video and follow the steps 


https://youtu.be/24TYp-vwnuI


- Using your photo of the gel create a standard curve in Excel/numbers or graph paper of distance travelled from the well of each of the standard proteins. The ladder used was a 1 kb ladder from Invitogen Cat 10787-026

https://tools.thermofisher.com/content/sfs/manuals/1kb_plus_dnaladder_1000ug_man.pdf

- Using the standard curve calculate the size of the plasmid and the two digest fragments from your samples  

- Are the results of the plasmid what you expected - look at the plasmid map to figure this out.

- Are the two fragments the sizes you expected

  • 8 years ago
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