lab report ASAP (2 PAGES)

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Objective, Background, method, result and conclusion (ALL IN PAST TENS )

ANSWER THE Questions.


SEE THE RESULTS IN EXCEL SHEET



 

  • a table of the data from the growth experiment 
    • Time in mins
    • count date from each group. 
    • average data 
    • actual dilution (remember the 0.1 ml added to the plate is another 10 fold dilution)
    • Cfu/ml (represent with one digit to the left of the decimal point and two to the right then the exponent)
    • Absorbance at 600nm 
    • Calculated CFU using the Aligent website 
  • GraphTitleY axisX axisE coli Growth CurveAbsorbance (600nm)Time (mins)E. coli Growth CurveLog bacterial numbers (CFU MM/ml) Aligent)TimeE. coli Growth CurveLog bacterial numbers (CFU MM/ml)TimeE coli Standard CurveAbsorbance (600nm)Cell numbers (MM/ml) for both Aligent and your CFU data
    • Image:MC4100 OD vs CFU curve.jpg

Questions

  • Look at the growth curves using Aligent CFU and your CFU: are they similar or different.  If they are different why is that..
  • Look at the aligent  plate count vs absorbance graph  - are they similar are they different.  If they are different why do you think that 
  • Look at the aligent and your cfu data vs time  - calculate the maximum time of doubling 
  • 8 years ago
  • 20
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