Lab report
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ResearchProjectLabReportGuidelines.pdf
Lab5part2.pdf
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ResearchProjectLabReportGuidelines.pdf
WRITING A LABORATORY REPORT (.doc/.docx or .pdf format only)
Each student has to write an individual lab report by themselves, it is not a group report, though they will share same experimental data/image collected during the research project labs.
PARTS OF THE LAB REPORT
Follow the lab project report guidelines but remember this guideline is not comprehensive- be attentive of the guidelines explained by TAs for modifications/alteration.
To write a lab report, referencing the laboratory manual/ Biology textbook is only a beginning. Seek out original sources, primary literature (peer-reviewed journal articles). Limit textbook citations, as the information is less reliable than primary literature. Use the library/e-library resources to find more detailed information on the topic. The completed laboratory report is to include the following sections as described: 1. TITLE PAGE
• Informative report title (it should describe lab content concisely, adequately & appropriately)
• Your full name
• Your email address (UNT email)
• Course and section numbers (BIOL1760/61.xxx)
• Date report submitted
• Laboratory instructor name (TA name)
2. ABSTRACT
Concise summary in one paragraph (200-400 words) that summarizes your report as follows:
• The scientific context of your experiment (what are you testing & why?)
• What you did
• How you did it (generally – NO specifics; do not write the method here)
• What you found (state your results qualitatively, not quantitatively)
• What it means- 1-2 sentence discussion and conclusion
3. INTRODUCTION
• Background information so that a reader will understand the purpose of your experiment
• Explain the purpose of the research project
• State the formulated hypotheses /rationale and predictions being tested when appropriate for the research project.
• Generally, 2 paragraphs long, 1st paragraph going over major concepts and key terms. While the second
paragraph addresses the experiment, questions it is trying to answer, and predicted outcomes. • Talk about the experiment; what are the procedures, what were the controls, independent and dependent
variables, what analysis or comparison do you plan on doing to your results etc. • Give a brief description of treatments used and what was measured.
• Generally, this part of the lab report will have the most references/citations- cite experiments from journals &
relate to your experiment.
4. MATERIALS AND METHODS
• Describe the procedure in your own words (so that the procedure could be replicated)
o Make sure to report any changes from the lab protocol!!!
• Methods and materials should be written separately and concisely.
• Details like concentrations (mM, g/L, etc.), temperatures, and sample size should be mentioned
• Tables can be used to show your different treatment groups (i.e. how you set them up) • No references/citations usually noted in this section.
5. RESULTS
• Open with a statement of the overall findings from the experiment
• Describe your results (point out trends or important features) and explicitly reference figures/images
• Insert tables/figures/graphs/images to describe your results (see below for details)
• Mention in the results, what were the numbers, the calculated data, tables, graphs, charts, any other visuals such as pictures of specimens, gels where applicable, etc.
FIGURES/TABLES/DIAGRAMS- Summarize your data into graphs and tables (in the results section) with headings,
labels and legends:
• Graph: Figure 1 to Figure X
o Include Figure legends (text below the Figure) so that it is not necessary to refer back to the report to
understand the Figure. Include information about methods (temperature, concentration), how the
data are expressed, sample size, and any abbreviations
o Label the axes and define all treatments (including units if appropriate)
o Make sure to include a title! o Make sure the graphs are easy to read and sized appropriately
o Pay attention to formatting. For example, the title of a graph should not be on a separate page from the graph itself.
• Table: Table 1 to Table X
o Use columns for categories of information (i.e. size, shape, etc.)
o Use rows for the different entries (i.e. species of bacteria)
o Include Table captions (text above the Table) so that it is not necessary to refer back to the report to understand
the Table. A caption presents a succinct statement of the contents of the table.
• In Molecular Biology Project- Images with proper labels
• No references/citations usually noted in this section.
6. DISCUSSION
• Open with a statement that either supports/rejects hypothesis or rationale
• Back up that statement by referring to findings from the experiment (briefly, do not re-state the same data
from the results section & do not refer to figures/tables)
• Generally, the number of paragraphs reflects the amount of data.
• Interpret your results (data/image) and relate to your initial rationale or hypothesis at the beginning of the
experiment
• Discuss any expected/unexpected findings regarding the hypothesis/rationale you made in the
INTRODUCTION section
• Address any issues that you encountered during the experiment (like problems/errors)
o Explain why they might have occurred
o Explain what you could do to fix the problem/error
• Critique the experimental design used
o Does it adequately address the hypotheses/rationale being tested?
o Were there faulty assumptions in the design that make interpretation of the data difficult?
o What new questions are prompted by the results? • Describe technical factors that you believe might help the reader interpret your data
• If there are no data (tables/graphs) interpret/discuss problems related to the hypothesis.
• In Molecular Biology Project- discuss the relevance of the data/result of the DNA extraction and Gel
electrophoresis
• What do your results mean? Make a claim if you can "this evidence supports the idea that…." But do not say “proves”.
• Can have references/citations to help back your claim
7. CONCLUSION
The summary of the result and discussion in concise manner.
8. REFERENCES
• This section should list the references using APA format (http://owl.english.purdue.edu/owl/)
• Make sure to have in-text citations in the Project Report (citations in the different parts of the Project- e.g.-
Introduction/Discussion part)
• Prepare a complete alphabetized (by first author's last name) list of references cited in your report at the end
of the report.
• At least, 2 primary journal article citations required!
• DO NOT use direct quotes, paraphrase the info from the article and cite the source
• DO NOT use lab reports or presentations found online. ONLY use peer-reviewed publications.
• The citation should be in your text as well as the reference section!
• Copying/pasting from the web/any other source is considered plagiarism & will result in a zero!
• For references, text book is only a beginning. Seek out original sources, primary literature (peer-reviewed journal articles). Limit textbook citations, as the information is less reliable than primary literature. Use the library to find more detailed information on the topic.
9. GROUP PROJECT TEAM/SELF EVALUATION FORM
Directions: Complete an evaluation for each member of your team, including yourself. Justify scores of 1 or 5 for any parameter.
Scoring Parameters: Rate each member of the team on the following scale for each parameter:
5 – Strongly Agree, 4 – Agree, 3 – Neutral, 2 – Disagree, 1 – Strongly Disagree
NAME Helped the team move forward on the project by contributing ideas or solutions
Performed his/her share of duties
Assisted others as needed and acknowledged contributions from other team members
Prepared as expected for the project
Worked well with other team members and resolved conflicts in constructive manner
Total
Self (Group member-1)
Group Member-2
Group Member-3
Group Member-4
COMMENTS OR EXPLANATIONS:
FORMATTING THE DOCUMENT
• The report is to be typewritten.
• Send in .doc. .docx or .pdf format only.
• One-inch margins
• Twelve-point font (Times New Roman/Calibri/Arial)
• Double spaced (to allow for comments)
• Bold or underline headings for each section to separate it from the paragraph
• DO NOT use the words: I, we, got, etc.
• DO NOT use contractions (i.e. – “don’t”; instead, write “do not”)
• Use active past tense (http://owl.english.purdue.edu/owl/)
• Form complete sentences & check spelling!! • Approximate length of the report –as specified by the TA
• Spelling and grammar are essential; if you do not use both wisely, your report will not
have good grade.
• For students requiring writing assistance, visit the UNT Student Writing Center website at http://www.unt.edu/writinglab/. Locations and hours are listed. (Can get 2 extra credit points if you email the TA a confirmation that you went to the UNT Student Writing Center for your research project lab report)
• While expected to be neat in presentation, the lab report will be graded primarily on the accuracy and
quality of technical content. Late submission Policy: Check the due date for your lab report in your Canvas sections. All submission assignments (e.g. research project lab report) will have five points deducted per day if submitted later than the due date. A grade of zero for the assignment will be given after 2 late days, regardless of the reason for non-submission.
Talk to your instructor in advance of the due date if circumstances beyond your control keep you from turning in a report on time. Otherwise, the instructor will have no other option but to assign a grade of zero for the report. Policy on Academic Misconduct:
The laboratory report is to be in the words of the individual student. Therefore, reports that appear to be the work of more than one person will receive a grade of zero for the assignment for all parties involved. No cheating will be tolerated. Anyone cheating (all forms) or exhibiting behaviors consistent with cheating, including, but not limited to, assisting other students with cheating: accepting credit for another’s work, etc., will receive as a minimum punishment a grade of zero for the graded item.
Plagiarism is presenting the work of another as your own or re-using your work for another class in this class without permission. Plagiarism will not be tolerated; the student will get a grade of zero for that assessment. In circumstances where a student copied another student’s work and the student that shared their work both will get a grade of zero for the graded item. Self-plagiarism is also considered plagiarism. Please make yourself knowledgeable about self-plagiarism issues. More severe punishment will be given depending upon the circumstances. All incidents of academic dishonesty will be reported to the appropriate authorities.
Lab5part2.pdf
LAB WORKSHEET FOR CELL RESPIRATION PROJECT (WITH VERNIER LQ)
Students should read the full “Cell Respiration Verner LQ “ in Canvas for detailed notes.
Using the CO2 and O2 Gas Sensors, you will monitor the carbon dioxide produced and the oxygen consumed by different specimens during cell respiration.
In this experiment, you will
• Use an O2 Gas Sensor to measure concentrations of oxygen gas. • Use a CO2 Gas Sensor to measure concentrations of carbon dioxide gas. • Study the effect of temperature on cell respiration. In this experimental set up we are trying to see the difference in rates of respiration for 1. Germinated peas and non-germinated 2. Germinated peas in room temperature, and cold temperature. 3. Germinated peas and Insects.
MATERIALS:
LabQuest Bio-Chamber 250 LabQuest App Ice cubes Vernier CO2 Gas Sensor Two 100 mL beakers Vernier O2 Gas Sensor Thermometer 25 germinated peas and other specimens Logger Pro (optional)
PROCEDURE/METHOD
1. Turn on the lab quest.
2. Do not try to connect to WIFI with the lab quest.
3. Measure the room temperature using a thermometer and record it in Table 1.
4. Obtain 25 germinated peas (or specimen provided in the lab) and blot them dry between two pieces of paper towel.
5. Place the germinated peas into the respiration Bio Chamber 250.
6. Connect the O2 Gas Sensor and the CO2 Gas Sensor to LabQuest.
7. Insert the Gas Sensors into the Bio Chamber 250 as shown in Figure 1. Insert the sensor snugly. The O2 Gas Sensor should remain vertical throughout the experiment. The CO2 Gas Sensor is horizontal in this experimental set up.
8. Make sure the units for CO2 and O2 gases are same (ppm).
9. Wait three minutes, to let the setting stabilize-then start data collection.
10. Data is collected for the amounts of CO2 and O2 gas in the Bio Chamber for duration of time of the experiment (See Tables 1A-C)
11. Do not touch or move the Bio Chamber/Lab Quest set up during data collection otherwise the data will be disturbed, and the graph will be jerky.
12. Record the data in different time intervals in the tables (1A-C) given below for each specimen.
13. When data collection has finished, remove the sensors from the respiration chamber. Take the specimens (e.g. peas) out.
14. Thoroughly dry the inside of the respiration chamber with a paper towel and store the Gas Sensors properly; O2 Sensor vertically and CO2 Sensor horizontally
15. Repeat Steps 5–14-substituting the germinated peas with other specimens provided (e.g. non- germinated peas etc.)
16. One you have collected all the data-put the collected data in Excel sheet in tabular form.
DATA TABLE:
Table 1
Condition Temperature (°C)
Room
Table 1A
O2
Rate of respiration (ppm/s)
CO2 Rate of respiration
(ppm/s)
Germinated Peas
0 min 3 min 6 min 9 min 12 min
0 min 3 min 6 min 9 min 12 min
Non-germinated Peas
0 min 3 min 6 min 9 min 12 min
0 min 3 min 6 min 9 min 12 min
Table 1B
O2
Rate of respiration (ppm/s)
CO2 Rate of respiration
(ppm/s)
Germinated Peas, room temperature
0 min 3 min 6 min 9 min 12 min
0 min 3 min 6 min 9 min 12 min
Germinated Peas, cool temperature
0 min 3 min 6 min 9 min 12 min
0 min 3 min 6 min 9 min 12 min
Table 1C
O2
Rate of respiration (ppm/s)
CO2 Rate of respiration
(ppm/s)
Germinated Peas
0 min 3 min 6 min 9 min 12 min
0 min 3 min 6 min 9 min 12 min
Insects
0 min 3 min 6 min 9 min 12 min
0 min 3 min 6 min 9 min 12 min
Comparing Different Respiratory Gases in Different Specimens
Table 2A
Germinated Peas Non-Germinated Peas
Time Interval
(min) O2 (ppm/s) O2 (ppm/s)
0
3
6
9
12
Table 2B
Germinated Peas Non-Germinated Peas
Time Interval (min) CO2 (ppm/s) CO2 (ppm/s)
0
3
6
9
12
Table 3A
Germinated Peas in Room Temperature Germinated Peas in Cool Temperature
Time Interval (min) O2 (ppm/s) O2 (ppm/s)
0
3
6
9
12
Table 3B
Germinated Peas in Room Temperature Germinated Peas in Cool Temperature
Time Interval (min) CO2 (ppm/s) CO2 (ppm/s)
0
3
6
9
12
Table 4A
Germinated Peas Beetles
Time Interval (min) O2 (ppm/s) O2 (ppm/s)
0
3
6
9
12
Table 4B
Germinated Peas Beetles
Time Interval (Min) CO2 (ppm/s) CO2 (ppm/s)
0
3
6
9
12
THOUGHT QUESTIONS
1. Do you have evidence that cell respiration occurred in peas? Explain.
2. What is the effect of germination on the rate of cell respiration in peas?
3. What is the effect of temperature on the rate of cell respiration in peas?
4. Why do germinating peas undergo cell respiration?
5. Compare the respiration rate among various types of seeds.
6. Compare the respiration rate among various types of small animals, such as insects or worms.