LAB report 12 HOURS( 2 PAGES)

DO NOT DO RESULT SECTION 

This lab report will cover the transformation of E.coli  and the restriction digest of the plasmid (labs 5 to 8).

Use the Standard lab report format and include the following 

- Overall objective 

- Background to what you were trying to do 

- Methods - used to create reagents and used for the transformation, plasmid isolation, restriction digest and agarose gels.  Create sub section for each 

Transformation

- What was the selectable marker?

- How do you know you successfully transformed the cells with pGlo

- What was the most critical step in the transformation process

Plasmid preparation

- Why does only the E. coli grown in ara/amp fluoresce

Restriction digest 

- What size fragments would you expect when cutting the plasmid with the two enzymes used? 

- Are the results of the plasmid what you expected.

- Are the two fragments the sizes you expected ?