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answer the question in excel following the order

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25.08.2024
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Work.xlsx

Work.xlsx

            order

                        The experiment found the expression of plant-Defence related genes in pathogenic infectious conditions and conditions in which beneficial microorganisms coexist with plants.
                    
                        Treatment 1	
                        neg	
                        Sterile tree
                    
                        Treatment 2	
                        Xcc	
                        The tree is infected with canker disease in lemon. (Xanthomonas citri pv citri)
                    
                        Treatment 3	
                        Pse	
                        A plant that has been planted with useful germs (Pseudomonas sp.)
                    
                        Gene of Interest: Plant-defense related gene
                    
                        LRRL	
                        leucine-rich repeat receptor-like protein gene
                    
                        RPP13L	
                        Disease resistance protein RPP13 gene
                    
                        CHSTL	
                        Chalcone synthase  Like gene
                    
                        BSK1L	
                        Brassinosteroid-signaling kinase 1 Like gene
                    
                        House keeping gene
                    
                        Ef1a gene (Elongation Factor 1 a Gene)
                    
                        question	
                        Find the expression of Plant-defense related gene (choose  1 gene) method ?
                    
                        Fold difference = 2^-ΔΔCt
                    
                        Fill the available values in the table.
                    
                        Create an easy-to-understand bar chart.
                    
                        Ready to explain / write a summary
                    
                        ANSWER	
                        Find average cq
                    
                        Find the SD and consider the statistics to use
                    
                        Find delta Ct
                    
                        Find delta delta Ct
                    
                        Find Fold difference = 2^-ΔΔCt
                    
            information 

                        Sample 6 h	
                        Ef1a gene (House Keeping Gene)	
                        	
                        RPP13L  gene	
                        	
                        ∆Ct 	
                        ∆∆Ct	
                        2^-∆∆Ct
                    
                        replicates for each	
                        	
                        cq rep. 1	
                        cq rep. 2	
                        cq rep. 3	
                        ave cq	
                        sd	
                        	
                        cq rep. 1	
                        cq rep. 2	
                        cq rep. 3	
                        ave cq	
                        sd
                    
                        1	
                        neg 1	
                        18.5	
                        18.53	
                        18.49	
                        	
                        21.67	
                        21.5	
                        21.8
                    
                        2	
                        neg 2	
                        20.34	
                        20.45	
                        20.4	
                        	
                        23.3	
                        23.6	
                        23.7
                    
                        3	
                        neg 3	
                        19.04	
                        19.18	
                        19.12	
                        	
                        22.48	
                        23.24	
                        22.96
                    
                        4	
                        neg 4	
                        20.13	
                        20.05	
                        20.06	
                        	
                        22.73	
                        22.8	
                        22.65
                    
                        5	
                        neg 5	
                        21.79	
                        22.37	
                        22.01	
                        	
                        24.87	
                        24.50	
                        25.02
                    
                        1	
                        xcc 1	
                        19.33	
                        19.31	
                        19.3	
                        	
                        22.13	
                        22.5	
                        21.87
                    
                        2	
                        xcc 2	
                        20.91	
                        21.15	
                        21.07	
                        	
                        24.14	
                        23.45	
                        23.7
                    
                        3	
                        xcc 3	
                        20.53	
                        20.34	
                        20.46	
                        	
                        22.75	
                        22.92	
                        23.41
                    
                        4	
                        xcc 4	
                        20.92	
                        20.87	
                        20.94	
                        	
                        22.03	
                        22.48	
                        22.64
                    
                        5	
                        xcc 5	
                        21.83	
                        22.26	
                        22.01	
                        	
                        23.78	
                        23.43	
                        23.5
                    
                        1	
                        pse 1	
                        20.92	
                        20.69	
                        20.73	
                        	
                        22.43	
                        22.82	
                        22.23
                    
                        2	
                        pse 2	
                        19.42	
                        19.50	
                        19.47	
                        	
                        21.38	
                        21.11	
                        21.65
                    
                        3	
                        pse 3	
                        20.60	
                        20.61	
                        20.58	
                        	
                        22.17	
                        22.56	
                        22.49
                    
                        4	
                        pse 4	
                        20.09	
                        20.53	
                        20.43	
                        	
                        22.04	
                        21.54	
                        22.16
                    
                        5	
                        pse 5	
                        23.31	
                        23.43	
                        23.35	
                        	
                        25.76	
                        25.40	
                        24

Work.xlsx

            order

                        The experiment found the expression of plant-Defence related genes in pathogenic infectious conditions and conditions in which beneficial microorganisms coexist with plants.
                    
                        Treatment 1	
                        neg	
                        Sterile tree
                    
                        Treatment 2	
                        Xcc	
                        The tree is infected with canker disease in lemon. (Xanthomonas citri pv citri)
                    
                        Treatment 3	
                        Pse	
                        A plant that has been planted with useful germs (Pseudomonas sp.)
                    
                        Gene of Interest: Plant-defense related gene
                    
                        LRRL	
                        leucine-rich repeat receptor-like protein gene
                    
                        RPP13L	
                        Disease resistance protein RPP13 gene
                    
                        CHSTL	
                        Chalcone synthase  Like gene
                    
                        BSK1L	
                        Brassinosteroid-signaling kinase 1 Like gene
                    
                        House keeping gene
                    
                        Ef1a gene (Elongation Factor 1 a Gene)
                    
                        question	
                        Find the expression of Plant-defense related gene (choose  1 gene) method ?
                    
                        Fold difference = 2^-ΔΔCt
                    
                        Fill the available values in the table.
                    
                        Create an easy-to-understand bar chart.
                    
                        Ready to explain / write a summary
                    
                        ANSWER	
                        Find average cq
                    
                        Find the SD and consider the statistics to use
                    
                        Find delta Ct
                    
                        Find delta delta Ct
                    
                        Find Fold difference = 2^-ΔΔCt
                    
            information 

                        Sample 6 h	
                        Ef1a gene (House Keeping Gene)	
                        	
                        RPP13L  gene	
                        	
                        ∆Ct 	
                        ∆∆Ct	
                        2^-∆∆Ct
                    
                        replicates for each	
                        	
                        cq rep. 1	
                        cq rep. 2	
                        cq rep. 3	
                        ave cq	
                        sd	
                        	
                        cq rep. 1	
                        cq rep. 2	
                        cq rep. 3	
                        ave cq	
                        sd
                    
                        1	
                        neg 1	
                        18.5	
                        18.53	
                        18.49	
                        	
                        21.67	
                        21.5	
                        21.8
                    
                        2	
                        neg 2	
                        20.34	
                        20.45	
                        20.4	
                        	
                        23.3	
                        23.6	
                        23.7
                    
                        3	
                        neg 3	
                        19.04	
                        19.18	
                        19.12	
                        	
                        22.48	
                        23.24	
                        22.96
                    
                        4	
                        neg 4	
                        20.13	
                        20.05	
                        20.06	
                        	
                        22.73	
                        22.8	
                        22.65
                    
                        5	
                        neg 5	
                        21.79	
                        22.37	
                        22.01	
                        	
                        24.87	
                        24.50	
                        25.02
                    
                        1	
                        xcc 1	
                        19.33	
                        19.31	
                        19.3	
                        	
                        22.13	
                        22.5	
                        21.87
                    
                        2	
                        xcc 2	
                        20.91	
                        21.15	
                        21.07	
                        	
                        24.14	
                        23.45	
                        23.7
                    
                        3	
                        xcc 3	
                        20.53	
                        20.34	
                        20.46	
                        	
                        22.75	
                        22.92	
                        23.41
                    
                        4	
                        xcc 4	
                        20.92	
                        20.87	
                        20.94	
                        	
                        22.03	
                        22.48	
                        22.64
                    
                        5	
                        xcc 5	
                        21.83	
                        22.26	
                        22.01	
                        	
                        23.78	
                        23.43	
                        23.5
                    
                        1	
                        pse 1	
                        20.92	
                        20.69	
                        20.73	
                        	
                        22.43	
                        22.82	
                        22.23
                    
                        2	
                        pse 2	
                        19.42	
                        19.50	
                        19.47	
                        	
                        21.38	
                        21.11	
                        21.65
                    
                        3	
                        pse 3	
                        20.60	
                        20.61	
                        20.58	
                        	
                        22.17	
                        22.56	
                        22.49
                    
                        4	
                        pse 4	
                        20.09	
                        20.53	
                        20.43	
                        	
                        22.04	
                        21.54	
                        22.16
                    
                        5	
                        pse 5	
                        23.31	
                        23.43	
                        23.35	
                        	
                        25.76	
                        25.40	
                        24

other Questions(10)

	The experiment found the expression of plant-Defence related genes in pathogenic infectious conditions and conditions in which beneficial microorganisms coexist with plants.
Treatment 1	neg	Sterile tree
Treatment 2	Xcc	The tree is infected with canker disease in lemon. (Xanthomonas citri pv citri)
Treatment 3	Pse	A plant that has been planted with useful germs (Pseudomonas sp.)
Gene of Interest: Plant-defense related gene
	LRRL	leucine-rich repeat receptor-like protein gene
	RPP13L	Disease resistance protein RPP13 gene
	CHSTL	Chalcone synthase Like gene
	BSK1L	Brassinosteroid-signaling kinase 1 Like gene
House keeping gene
	Ef1a gene (Elongation Factor 1 a Gene)
question	Find the expression of Plant-defense related gene (choose 1 gene) method ?
	Fold difference = 2^-ΔΔCt
	Fill the available values in the table.
	Create an easy-to-understand bar chart.
	Ready to explain / write a summary
ANSWER	Find average cq
	Find the SD and consider the statistics to use
	Find delta Ct
	Find delta delta Ct
	Find Fold difference = 2^-ΔΔCt

	Sample 6 h	Ef1a gene (House Keeping Gene)					RPP13L gene					∆Ct	∆∆Ct	2^-∆∆Ct
replicates for each		cq rep. 1	cq rep. 2	cq rep. 3	ave cq	sd	cq rep. 1	cq rep. 2	cq rep. 3	ave cq	sd
1	neg 1	18.5	18.53	18.49			21.67	21.5	21.8
2	neg 2	20.34	20.45	20.4			23.3	23.6	23.7
3	neg 3	19.04	19.18	19.12			22.48	23.24	22.96
4	neg 4	20.13	20.05	20.06			22.73	22.8	22.65
5	neg 5	21.79	22.37	22.01			24.87	24.50	25.02
1	xcc 1	19.33	19.31	19.3			22.13	22.5	21.87
2	xcc 2	20.91	21.15	21.07			24.14	23.45	23.7
3	xcc 3	20.53	20.34	20.46			22.75	22.92	23.41
4	xcc 4	20.92	20.87	20.94			22.03	22.48	22.64
5	xcc 5	21.83	22.26	22.01			23.78	23.43	23.5
1	pse 1	20.92	20.69	20.73			22.43	22.82	22.23
2	pse 2	19.42	19.50	19.47			21.38	21.11	21.65
3	pse 3	20.60	20.61	20.58			22.17	22.56	22.49
4	pse 4	20.09	20.53	20.43			22.04	21.54	22.16
5	pse 5	23.31	23.43	23.35			25.76	25.40	24