bio tech

1. The authors of this      paper have converted Müller glia into retinal ganglion cells by reducing      the expression of what gene?

QUESTION 2

1. The model used in the      paper involves causing retinal injury to mice. Briefly describe the method      for inducing this injury.

QUESTION 3

1. Ptbp1 is found in      numerous cell types. What feature of the construct(s) used allowed      knockdown of Ptbp1 specifically in Müller glia?

10 points   

QUESTION 4

1. Retinal ganglion cells      receive visual information from photoreceptors through the bipolar cells      and retina amacrine cells, and transmit visual information (as action      potentials) to what regions in the brain?

10 points   

QUESTION 5

1. Parkinson's disease is      associated with the degeneration of what kind of neurons ,      in what region of the brain ?

10 points   

QUESTION 6

1. Briefly describe the      role of Oxidopamine (6-OHDA) in the experiment(s) described. What sort of      cells does it affect?

10 points   

QUESTION 7

1. What is the major      function of the enzyme tyrosine hydroxylase? 

10 points   

QUESTION 8

1. - What feature of the      tyrosine hydroxylase-expressing cells in the experimental mouse brains      suggests they changed cell type?
   
        - How does it suggest that?
   
        - What cell type were they (probably) originally?

10 points   

QUESTION 9

1. What is the clinica      significance of dopaminergic neurons in terms of human diseases?

10 points   

QUESTION 10

1. What is the significance      of ipsilateral and contralateral rotation behavior? What drug(s) are used      to induce these behaviors?