Score for this attempt: 80 out of 80
Submitted Feb 19 at 6:29pm
This attempt took 81 minutes.
Question 1
2 / 2 pts
All cellular RNAs are transcribed from ________.
DNA templates
RNA templates
either DNA or RNA templates, but not both in the same organism
a combination of DNA and RNA templates
none of the above
Question 2
2 / 2 pts
What type of modification is used to make CpG islands?
methylation of guanine
acetylation of guanine
phosphorylation of guanine
acetylation of cytosine
methylation of cytosine
Question 3
2 / 2 pts
DNA polymerase from Thermus aquaticus is used in PCR because ________.
the enzyme is readily deactivated by heat, effectively halting the reaction
it is stable at high temperatures
the genes from Thermus aquaticus are readily distinguished from those of
"normal" organisms
it is a soluble protein
it is not infectious
Question 4
2 / 2 pts
In most organisms, replication proceeds in a ________ manner from the
________.
bidirectional; lagging strand
bidirectional; replication origin
bidirectional; theta site
unidirectional; chromosome ends
none of the above
Question 5
2 / 2 pts
Which of the following best describes a new strand of DNA relative to the
template strand used to synthesize it?
a palindromic copy of the template
an exact duplicate of the template
an exact copy of the template but with the 3' and 5' ends reversed
a negative copy of the template
none of the above
Question 6
2 / 2 pts
What reagents are required to perform PCR?
DNA fragment, primers flanking the region of interest, dNTPs, ddNTPs, DNA
polymerase
DNA fragment, primers flanking the region of interest, dNTPs, DNA
endonuclease
DNA fragment, one primer, dNTPs, DNA polymerase, DNA endonuclease
DNA fragment, one primer, dNTPs, DNA polymerase, DNA ligase
DNA fragment, primers flanking the region of interest, dNTPs, DNA
polymerase
Question 7
2 / 2 pts
Which of the following best describes the functioning of the lac repressor
protein?
in the presence of allolactose, the protein binds the operator inhibiting
transcription
in the absence of allolactose, the protein binds the operator inhibiting
transcription
in the absence of allolactose, the protein binds the operator enhancing
transcription
in the presence of allolactose, the protein binds the operator enhancing
transcription
none of the above
Question 8
2 / 2 pts
Synthesis of telomeres requires the enzyme ________, which is a type of
________.
telomerase; reverse transcriptase
ribonuclease; RNA-specific nuclease
telomerase; helicase
ribotelomerase; ligase
none of the above
Question 9
2 / 2 pts
Which of the nucleosome proteins is found in the linker DNA between
nucleosomes and not actually part of the nucleosome core?
H1
H2A
H2B
H3
H4
Question 10
2 / 2 pts
A gene knockout is ________.
a gene present on a YAC
a gene that has been inactivated or removed from an organism
a dominant gene that knocks out expression of other genes
a gene inserted in place of another gene
none of the above
Question 11
2 / 2 pts
Histone proteins that bind to areas of DNA that are highly transcribed are
often ________ on ________ residues.
phosphorylated; tyrosine
acetylated; lysine
phosphorylated; serine
acetylated; histidine
methylated; lysine
Question 12
2 / 2 pts
In DNA, the ribose derivative lacks an ________ on C-________.
amine; 3
amine; 2
alcohol; 3
alcohol; 2
none of the above
Question 13
2 / 2 pts
What does allolactose bind?
lac repressor
promoter
lac operon
operator
all of the above
Question 14
2 / 2 pts
Which of the eukaryotic transcription factors is responsible for binding the
TATA box?
TFIID
TFIIH
TFIIF
TFIIE
TFIIB
Question 15
2 / 2 pts
Which of the following is the major factor in determining how quickly an
mRNA is degraded?
degree of intron removal
length of poly(A) tail
overall length of the mRNA
how quickly the cap is removed
all of the above play a role
Question 16
2 / 2 pts
In a typical gene, what percentage of the bases represents introns?
50%
10%
90%
5%
25%
Question 17
2 / 2 pts
What protein/RNA complex is responsible for the removal of introns?
ribozyme
intronase
RNA processing complex
spliceosome
none of the above
Question 18
2 / 2 pts
The DNA strand that serves as the template for the synthesis of RNA is often
called the ________.
noncoding strand
coding strand
transcription strand
transfer strand
messenger strand
Question 19
2 / 2 pts
Which of the following is thought to be the origin of RNA interference?
removal of mistranscribed mRNA
removal of excessive mRNA
antibiotic for RNA based bacteria
antiviral defense
none of the above
Question 20
2 / 2 pts
Which transcription factor is required to aid RNA polymerase in the removal
of an incorrect nucleotide incorporated into the growing RNA?
TFIIX
TFIIZ
TFIIP
TFIIT
TFIIS
Question 21
2 / 2 pts
Eukaryotic transcription termination generally occurs at ________ sites in the
DNA sequence.
stop
CpG island
exonuclease binding
pause
disconnect
Question 22
2 / 2 pts
Without telomeres, what would happen to the ends of eukaryotic
chromosomes during replication?
they would be replicated just like the rest of the chromosome
they would be lengthened
they would be mutated
they would be shortened
none of the above
Question 23
2 / 2 pts
What sugar is metabolized by the enzymes under the control of
the lac operon?
lactose
sucrose
maltose
mannose
galactose
Question 24
2 / 2 pts
What effect does a topoisomerase have upon DNA?
converts double stranded DNA to single stranded DNA
changes the supercoiling of DNA
changes B-form DNA to A-form
denatures DNA
none of the above
Question 25
2 / 2 pts
In living organisms, genetic information is most often stored in the form of
________.
ribonucleic acid
enzymes
deoxyribonucleic acid
deoxynucleotides
proteins
Question 26
2 / 2 pts
Which of the following would likely result in the formation of RNA?
injection of antisense RNA from yeast into a human
injection of antisense RNA from a human into human cells
injection of sense RNA into C. elegans
all of the above
none of the above
Question 27
2 / 2 pts
What term describes the production of multiple identical organisms from a
single ancestor?
cloning
ligation
sequencing
phenotyping
transcription
Question 28
2 / 2 pts
During termination, Rho acts as a ________.
ligase
NTP hydrolase
single-strand binding protein
helicase
polymerase
Question 29
2 / 2 pts
What DNA sequence is found on the template strand at the termination of
prokaryotic transcription?
series of CG repeats followed by string of 10-12 U
section of Z-DNA followed by repetitive CAT sequence
series of TATA boxes
series of CpG islands
palindromic sequence followed by poly(A) region
Question 30
2 / 2 pts
What term describes a small, circular molecule of DNA that can be used to
transfer genetic material from one organism to another?
tRNA
bacteriophage
splice
clone
plasmid
Question 31
2 / 2 pts
What technique involves addition of primers that do not exactly match the
sequence of a gene, thus allowing for introduction of a mutation?
transformation
selection
cloning
site-directed mutagenesis
none of the above
Question 32
2 / 2 pts
Which of the following is found in high concentration in all telomeres?
U
C
G
A
T
Question 33
2 / 2 pts
What is the region immediately adjacent to the starting site for RNA
polymerization called?
enhancer
initiator
TF binding site
consensus sequence
promoter
Question 34
2 / 2 pts
Which of the following represents the correct order of steps in a PCR reaction
beginning with double-stranded DNA?
strand separation, primer extension, primer annealing
strand separation, primer annealing, primer extension
primer annealing, strand separation, primer extension
primer extension, primer annealing, strand separation
primer annealing, primer extension, strand separation
Question 35
2 / 2 pts
Genomic DNA that encodes proteins undergoes the process of ________ to
produce ________.
transcription; tRNA
translation; rRNA
transcription; proteins
translation; proteins
transcription; mRNA
Question 36
2 / 2 pts
Which of the following best describes the nucleic acid complex formed during
RNA polymerization?
one DNA-RNA hybrid with the other DNA strand bound to an RNA molecule
on RNA polymerase
one DNA-RNA hybrid and one single stranded DNA
two DNA-RNA hybrids since both DNA strands are transcribed simultaneously
there is no interaction between the growing RNA and the DNA
none of the above
Question 37
2 / 2 pts
In the nucleus, DNA is wrapped around ________ to form ________.
polyarginine; 30-nm fibers
polyribose; euchromatin
histones; nucleosomes
ribosomes; heterochromatin
none of the above
Question 38
2 / 2 pts
Besides the obvious role of keeping DNA in a single strand state, what do
single-strand binding proteins do?
aid DNA polymerase with DNA binding
protect single stand DNA from nucleases
aid in the formation of hairpin structures
prevent supercoiling
all of the above
Question 39
2 / 2 pts
What is the process used for genes shuffling in meiosis that is also used to
repair double-strand breaks?
nonhomologous end-joining
telomerase-assisted ligation
non-mutagenic strand coupling
homologous end-joining
recombination
Question 40
2 / 2 pts
Which strand of DNA is replicated exclusively in a discontinuous fashion?
forward strand
leading strand
the strand that is read in a 5′ to 3′ direction
reverse strand
lagging strand
Quiz Score: 80 out of 80
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Time: 81 minutes
