Worksheet5-DiffusionOsmosisEnzymes.docx

Lab 5 Diffusion, Osmosis and Enzyme Activity

THERE IS A SEPARATE INSTRUCTION SHEET FOR THIS LAB THAT YOU MUST READ.

I hereby declare that I performed the following experiments and all answers are my own.

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Name (typed)

Exercise 1

1.) Record results

Initial weight of bag (oz/g)

Final weight of bag (oz/g)

Change in weight (oz/g)

Time __________

Time ____________

Elapsed time ___________(minutes)

2.) Define diffusion –

3.) Why did the weight of the bag change?

4.) Upload photo for Exercise 1 here.

Exercise 2

1.) Record results

Observations of potato in water (cup 1)

Observations of potato in salt water (cup 2)

2.) Define osmosis –

3.) What is an isotonic solution?

Hypertonic solution?

Hypotonic solution?

4.) Is water (cup 1) a hypertonic or hypotonic solution?

Did water enter or leave the potato cell?

Is salt water (cup 2) a hypertonic or hypotonic solution?

Did water enter or leave the potato cell?

5.) Upload photo for Exercise 2 here.

Exercise 3

Using the above reaction answer the following questions:

1.) What is the enzyme?

What is the substrate?

What is the product?

How would you know if the product is made and the reaction occurred?

Experiment 3-1. Adding hydrogen peroxide

1.) Describe your observations here.

2.) Did the reaction occur?

3.) Upload photo for Experiment 3-1 here.

Experiment 3-2. Adding sucrose.

1.) Describe your observations here.

2.) Did the reaction occur?

If not, explain why it did not occur?

5.) Upload photo for Experiment 3-2 here.

Experiment 3-3. Adding vinegar.

1.) Describe your observations here.

2.) Did the reaction occur?

If not, explain why it did not occur?

3.) Upload photo for Experiment 3-3 here

Experiment 3-4. Adding heat.

Describe your observations here.

Did the reaction occur?

If not, explain why it did not occur?

5.) Upload photo for Experiment 3-4 here.

Once you have completed the worksheet you need to submit it using Blackboard.

Take Lab Quiz 5. This quiz covers the information from sections 4.9 and 5.4.