Micro Biology Lab Packet
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Week1-Day1IntroOrientationandGloGerm1-18-18.pptWelcome to General Microbiology Lab
Biol 2421
Introductions
- My name is Professor Troy Giambernardi
- Pronounced “Gim-burr-nardi”
- Please introduce yourself to the students at your table.
- Name, program of study, how long you’ve been a student at Lone Star College, how & how far you come to get here
- Class list: Please tell me your name, using the name you would like to be called
- Some information, please: Student Information Sheet
- Next lab, I’ll circulate a seating chart
Syllabus and Schedule are posted on Desire 2 Learn (D2L)
- Class & Lab will use the same D2L site
- Lab Syllabus
- Lab Schedule
Safety Considerations
- Respect live microorganisms!
- Please let me know later if you are immunocompromised or immunosuppressed
- No food or drink in the laboratory
- Lab coat, smock, or old shirt is highly recommended
- Close-toed shoes are highly recommended
- You are responsible for your own Gloves
- Must cover any cuts/open sores with gloves or band-aid
- Other safety items
- Raise any safety concerns or questions
Procedure for Disinfecting a Lab Spill
- Cover generously with Lysol®
- Lay paper towels on top
- Let sit for 5-10 minutes
- Clean it up
- Wear gloves and/or use brush & dustpan
- Be cautious of broken glass
- Discard into biohazard trash
Basic Rules & Procedures for Working in the Biology Laboratory
- Please sign Basic Rules and Procedures Sheet circulating around the lab
- Put on the instructors table up front
- A duplicate copy is at the back of the class syllabus on D2L
Before you come to lab
- Please look at scheduled activities on the lab schedule in the syllabus
- Please read the lab exercises in the Lab Manual
- Microbiology Laboratory Theory & Application, 4th edition; Leboffe & Pierce
- Please bring the lab manual with you to lab.
Summary of how lab runs:
- Put away gum, drinks, cell phones, etc.
- Disinfect bench with Lysol.
- Listen to short introduction – take notes.
- Do the lab exercise(s).
- Complete “Data Sheet” questions at the back of each section of your lab manual germane to the Exercise(s) we are working on.
- These will be collected along with the posted Lab Packet questions for grading.
- Disinfect lab bench.
- Wash hands before you leave.
Exercise 1-1
Glo Germ Hand Wash
Glo Germ Hand Washing
- Purpose:
- To assess proper hand washing techniques
- To keep the lab environment from becoming contaminated
- To keep us from becoming infected by our cultures
- Follow protocol from instructor
Inoculating Petri Plates from Environment Cultures
Step 1: Obtain a sterile cotton swab, sterile saline, and one agar plate.
Step 2: Choose a surface to sample.
Step 3: Label the bottom of your plate with sample name, your name or initials, and date.
Step 4: Remove sterile swab, wet swab with saline, and sample surface of interest with wetted swab.
Inoculating Petri Plates
Step 5: Holding the petri dish lid at an 30°-45° angle, work the contaminated swab from the outside of the plate toward the center in a zig-zag pattern that covers approximately 25% of the plate surface (think pie or pizza slice!).
Inoculating Petri Plates
Step 6: Turn the petri plate 90° to the right, dragging the inoculation loop through the last section of the plate, moving from the outside to the inside in a zig-zag motion.
Step 7: Repeat this process twice more until the entire plate surface is covered.
NOTE: If you are trying to isolate individual colonies, each turn of the dish will give you fewer microbes so that you can distinguish individual colonies.
If you streak your plates correctly, the pattern should look like this on your plate.
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If you streak your plates correctly, the pattern should look like this on your plate.
Inoculating Petri Plates
Step 8: Replace plate lid and invert the plate for incubation at 37°C.
Plates are inverted (upside down) to avoid condensation spilling onto your cultures.
