Bioengineering/matlab

Finding Km Values

http://www.brenda-enzymes.org/

Finding Km Values

• BRENDA – Kinetic activity database – Catalogs enzyme activity and other

kinetics-focused papers – EC Number

• #.#.#.#(##) – Identifies enzyme – Not species specific

– Can also search for substrates & ligands

Ex: Find Km for Succinyl-CoA Synthetase

Tissue-Specific in Humans GDP-Forming: Anabolic Metabolis ADP-Forming: Catabolic Metabolis