Genetics Worksheet

updatedDNAworksheet-Copy.docx

Part I - Name the enzyme

1. synthesizes RNA
2. elongates chains of DNA
3. seals nicks in DNA chains
4. cuts DNA at specific nucleotide sequences
5. uncoils DNA supercoils
6. binds to the promotor of an operon
7. synthesizes DNA
8. Recoils new DNA strands

Part II—Match the term with the appropriate phrase.

	Phrase	Term
	An agent that causes a change in the order of nucleotides in the DNA of a cell	a. Polymerase Chain Reaction
	Drugs used in the treatment of cancer and viral infections that causes an A-T pair to convert to C-G.	b. Hydrogen bonds
	An agent that causes breaks in the sugar-phosphate backbone of DNA causing translocations	c. Antiparallel

	An agent that causes covalent bonds to form between adjacent thymine resulting in mutations	e. Base analogs
	The forces that hold the double stranded DNA together	f. intron

	A process whereby DNA amounts can be amplified from a tiny drop to obtain a multitude of information	g. Ultraviolet light
	The orientation of paired DNA strands giving a 5’to 3’ and 3’ to 5’ configuration	h. mutagen
	Extra pieces of DNA that are cut out of RNA in eukaryotes	d. X-rays

Protein Synthesis

Directions: Determine the mRNA sequence using the DNA template provided. Then determine the amino acid sequence in the protein using the codon chart.

5’ TAC AAA GGA CCT TTT AAC CCC ACT 3’ DNA Template

______________________________________________mRNA

_________________________________________Protein

DNA strand TAC- GGC- CCA- TTA- AGG- GCA

mRNA sequence

Amino Acid seq.

Now rewrite the DNA, mRNA and the resulting A.A. sequence when considering the following mutations: (also what kind of mutations are these?)

1) In the DNA above insert or add a “G” between the 3rd and 4th codon

New DNA

New mRNA

New A.A.’s

2) In the original DNA delete the “A” from the 4th codon

New DNA

New mRNA

New A.A.’s

3) In the original DNA, substitute a “T” in the first position of 2nd codon

New DNA

New mRNA

New A.A.’s