Unknown project (microbiology)
sbudha
UnknownResultsandBiochemicalChart2.docxUnknown Project Results Sheet Unknown #____
Gram Reaction ________________
Acid-fast Reaction ________________
Endospore ________________
Cell morphology ________________
and arrangement
Description of the colony:
Color _________________
Unique Characteristics ___________
Growth Characteristics:
Oxygen requirements? _____________
Biochemical tests: (+ or -)
Catalase _______ Phenylalanine deaminase ________
Oxidase _______ Decarboxylase tests:
Indole _______ Arginine ________
Methyl Red _______ Lysine ________
Voges-Proskauer _______ Ornithine ________
Citrate _______ Casein hydrolysis ________
Motility _______ Starch hydrolysis ________
Hydrogen sulfide _______ Lipid hydrolysis ________
Phenol red sugars: DNase ________
Glucose (gas) _______
Glucose (no gas) _______
Lactose _______
Sucrose _______
Mannitol _______
Gelatin hydrolysis _______
Urea hydrolysis _______
Nitrate reduction _______
The bacterium was identified as ____________________
(Genus and species)
Add description of your unknown here:
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Name of test |
Purpose/Biochemical Reaction |
Name of Medium |
Indicator or Reagent |
Positive Reaction (visible reaction) |
Negative Reaction (visible reaction) |
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Casein hydrolysis |
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Catalase test
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N/A |
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Citrate test
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Blue slant |
Green slant |
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Deaminase test
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Decarboxylase test |
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DNAse
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Gelatin hydrolysis |
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H2S production
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Indole test
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Lipid hydrolysis
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Methylene blue (indicator) |
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Methyl red test
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Motility (from TTC) |
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Nitrate reduction |
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Oxidase test
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N/A |
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Phenol red sugar broths (various sugars) |
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Starch hydrolysis
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Urea hydrolysis
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Voges-Proskauer |
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