Micro-Unknown Research

Unknown Code: 322

Required Tests:

Growth on TSA

Figure 1. Colonies on TSA after 48 hours of growth at room temperature: colonies appear orange/red in color, 1 mm to 2 mm in diameter, round, dome-shaped, smooth, glossy, with straight edges.

Gram Stain

Figure 2. Cell morphological features, Gram stain: pink-stained cells that appear to have both rod- shaped cells and some small circular- shaped cells.

Oxidase Test

Figure 3. Oxidase test: no color developed within 5 seconds of adding bacterium to reagent

MacConkey Agar

Figure 4. MacConkey agar incubated at 28 o C: Shows growth and colonies appear red.

Figure 5. MacConkey agar incubated at 37 o C: Shows growth and colonies have no color.

Phenol Red Glucoae Broth

Figure 6. Phenol Red Glucose Broth at 24 hours: Incubated at 37 o C for 24 hours. Inoculated media is

turbid and yellow. The small bubble in the durham tube is the same size as the bubble in the control tube. Control = uninoculated tube.

Phenol Red Carbohydrate Broths

Figure 7. Phenol Red Fermentation Broths: Incubated at 37 o C for 24 hours. Inoculated phenol red

sucrose broth is turbid and yellow. Inoculated phenol red lactose broth is turbid and pink. Inoculated phenol red mannitol broth is turbid and pink. Control = uninoculated tube.

Sucrose Lactose Mannitol

Triple Sugar Iron Agar

Figure 8. Triple Sugar Iron Agar: Incubated at 37 o C for 24 hours. Inoculated media is solid with a yellow

slant and yellow butt. Control = uninoculated tube.

Motility

Figure 9. Motility Agar: Incubated at 37 o C for 48 hours. Inoculated media is clear and stab mark is red.

Control = uninoculated tube.

Control

Required Follow-Up Tests:

Nitrate Test

Figure 10. Nitrate broth at 48 hours after addition of Reagent A and Reagent B. No zinc added: inoculated media is red. Control = uninoculated tube.

Urea Agar

Figure 11. Urea Broth: Incubated at 37 o C for 48 hours. Inoculated media is turbid broth is a organish-

red color. Control = uninoculated tube.

Control

Citrate Agar

Figure 12. Citrate Agar: Incubated at 37 o C for 48 hours. Inoculated media is blue. Control =

uninoculated tube.

control