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9/19/18, 4(12 PMThread: DB #7 – BIO-101-1-Fall-2018-CRN-20168

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BIO- 101- 1- Fall- 2018- CRN- 20168 PRINCIPLES OF BIOLOGY & LAB

Discussion Board Forum: Week 5 Discussion Forum Thread: DB #7H

Thread: DB #7

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Anonymous

DB #7

1 month ago

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Select and summarize two of the short articles linked under “Broad News” on the CRISPR Research News page. (https://www.broadinstitute.org/project-spotlight/crispr-news- and-press-releases)

RE: DB #7

1 day agoSara Smith

The first article I chose to read was titled, "Reading the rule of gene regulation with CRISPR". This article started off by discussing the rules that go into genome protien-coding components. For instance, we know where it begins, it ends, and the pieces of it's geography. However, the part where that dictates which genes the cell reads, we do not know this much about. This is where CRISPR could come in and help. CRISPR in correlation to gene editing, could lend a helping hand in grasping these unknown rules. In two different approaches, using CRISPR to locate these unkown rules, led to finding and locating several genetic regulators. From locating these genetic regulators lend,

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My Institution My Courses My ContentHamad Alzahid

Discussion Prompt:

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9/19/18, 4(12 PMThread: DB #7 – BIO-101-1-Fall-2018-CRN-20168

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the scientist to focus on the sequence that was being created. CRISPR was able to probe these sequences directly, unlike they were able to do before. IN addition, CRISPR was able to look at these sequences within their native habits, rather than having to remove them. In conclusion, CRISPR allowed for these scientist to look at the hidden side of genetics and its rule, which was not prominent before.

The second article I chose to read was titled, "Smile for the camera, CPf1". This article was based around a picture of the first 3D atomic resolution structure of a CRISPR enzyme called Cpf1, and embracing RNA strand and a target DNA strand. This picture produces some accounts for differences in the Cas9 and Cpf1. In additions, gives another view of how CRISPR enzymes can precisley target and cut DNA. In addition, this view allows for scientists, to be able to more properly identify how CRISPR can effect DNA. This picture can ultimately allow for more procedure s and hypothesis to be created because it gives one a visual perspective.

RE: DB #7

18 hours agoErin Pyle

In the first article, "Scientists unveil CRISPR-based diagnostic platform" in 2017 states the unveil of CRISPR based on a diagnostic platform. To further explain, this means that scientists have developed a tool in order for them to be able to diagnose patients. The goal of the tool is to be able to provide a diagnostic tool that is inexpensive and has the power to greatly transform research and the global public health. These diseases they are able to diagnose involve detecting antibiotic-resistant genes, e Coli bacteria, potential Zika virus in patients blood or urine and many more. By having an enzyme that will target the direct area, they are able to pinpoint the presence of an RNA or DNA molecule. The new tool called “Sherlock” has the ability to be aware of potential vital outbreaks as mentioned along with cancer.

The second article, "SHERLOCK advances its CRISPR-based diagnostic tool" 2018, clarifies the goal of the “Sherlock” device as testing and research have been able to back the information. By allowing a team of scientists to research and study the device, they

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9/19/18, 4(12 PMThread: DB #7 – BIO-101-1-Fall-2018-CRN-20168

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have come to the ability to recognize pathogens, tumor DNA and or any genetic signature. With that being said, this ability allows medical professionals to use a simple paper strip test to uncover viruses such as Zika. By using the “Sherlock” medical professionals have the accessibility to an inexpensive and affordable device that is able to detect nucleic acid which relates to viruses, tumor DNA, and other targets. The team of researchers have been able to successfully recognize various cancerous DNA and or viruses that can cause potential damage. As a result of the testing, they are able to help prevent further damage and seek the correct course of treatment depending on the individuals' needs. Science has come a long way as far as being able to recognize treatment and understanding diseases. With the “Sherlock” and CRISPR process, many lives are able to be saved as a result of being treated and uncovered by the genetic process and testing.

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