assignment
Bella edwards
Su20PosterGuidelinesOnly_revRST2.pptxPoster instructions
Poster, in general
The sections of the poster are:
Introduction
Hypothesis
Results
Conclusions
Future Directions
Literature Cited
I have put these on separate slides on the handout so you can see them more closely, but you should put them in a scientific poster format (48 inches wide, 36 inches tall)
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Introduction or Background
This should contain the revised introductory and hypothesis material from Writing Assignment 2.
You can use bullet lists or paragraphs.
You can also use images, but be sure to cite them!
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Results
This section should include a description of the bioinformatics discoveries that you made and all figures that relate. At a minimum the results should include your “story” of the process that we have used to approach your ‘experiment’.
Example figures are shown on the following 5 slides and are the minimum that should be included.
All figures need figure titles and legends. They go below the figure.
Tables (if you use a table) also need a table title. The title goes above the table.
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Results: exon/intron diagrams
The gene structure for your mRNA isoforms. This should be cited as being from Ensembl release 100 - April 2020
Citation (put it in CSE format): Sarah E Hunt, William McLaren, Laurent Gil, Anja Thormann, Helen Schuilenburg, Dan Sheppard, Andrew Parton, Irina M Armean, Stephen J Trevanion, Paul Flicek, Fiona Cunningham Ensembl variation resources Database Volume 2018 doi:10.1093/database/bay119.
The figure (above) is compiled from the “snips” of the transcripts in Ensmbl. You should label it clearly as male/female and isoform number. Include a legend describing the features (boxes, lines,colors)
Olfr554 Isoforms
Male, -202
Female, -203
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Results –multiple alignment
Alignment of the two isoforms
If your splicing was in the UTR, you will put the regulatory RNA diagram here.
Translation software (ExPasy) and alignment software (clustal and boxshade) should be cited.
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Your alignment may be VERY large. You can use a whole column on the poster if needed. You may also highlight the area where the difference(s) is (are). –Talk to Dr. Taylor about how to do this.
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Results – Domain structure
Domain structure of your protein isoforms, mark those you found
Everyone should have this, even the regulatory RNA folks. They just won’t have isoform below their diagram.
Domain search software should be cited.
RefSeq
Isoform 1
If this program gives no results, you should use the alternate directions for domain searching.—CONTACT DR. TAYLOR
If still no results, just put that in your results section text.
Note: making a truncated/nonfunctional protein is a regulatory mechanism by which the cell can effectively reduce or eliminate the functional product (protein) without having to alter transcription of the gene – it makes for a quick response…
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Results: Comparison Tables
| Feature | Mouse |
| Gene name | |
| Gene stable ID | |
| Chromosome | |
| Refseq mRNA ID | |
| Description |
Table 1: Details on _____________________(your gene of interest)
| Feature | Increased expression in Males | Increased expression in Females |
| Transcript name | ||
| Ensembl Transcript ID | ||
| Transcript start | ||
| Transcript end | ||
| Transcript length | ||
| Number of exons | ||
| Number of amino acids | ||
Table2: Features of _______________ gene expression in the genital ridge of Mus musculus at day E11
Note that I have removed the human column from this table, since we did not have time to include that protocol-
HOWEVER! If you were able to find that information, or some of it, then please include it and use it in your conclusions, if you wish.
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Conclusions
This will be a major focus of the poster draft review- you will be able to revise it before the final version.
a. Make a list of the big conclusions you can make from your results and then order them according to how it makes sense to present them. The order does NOT have to be the order for your results section. but often it is… think about how to best tell your story. At a minimum, you will need to make clear the answers to the following questions:
What was the nature of the alternative splicing differencesbetween your isoforms? (protein-coding-exon skipping, UTR-exon skipping (and 5’ or 3’?) was it intron retention?)
Do the predicted RNAs make different proteins?
What conclusions might you make regarding alternate proteins that are made – do you think they will be functional or function differently based on the domains present/absent?
What might the functionality of the proteins indicate for development of the tissue in the sex in which they are expressed?
What does the known function of the gene product suggest about its function at this early stage of sex development, if any?
b. You’ll want to do some literature searching here to give context and find similar/different results from others in the field. Each person’s integration of their results into the current literature will be different and depend on your conclusions and the literature.
c. You’ll want to end the conclusions by drawing the reader back out to the big picture of this project, that these genes may be involved in sex determination/ differentiation.
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Future Directions
This also will be a major focus of the poster draft review- you will be able to revise it before the final version.
At a minimum, you will need to make clear the answer to the following questions and suggest at least 2 future directions:
What results from your experiment should be followed up on? And how?
Can you suggest a specific hypothesis that could be studied in follow-up? (laboratory or bioinformatics)
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Literature Cited
You will need to use CSE format as we have been doing this entire semester. In-text citations are expected in all sections using the name-year format. Full references of all in text citations should be in the Literature Cited in alphabetical order in CSE format.
NB: these slides are adapted from those developed by Dr. Seipelt-Thieman for the regular semester-long course
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