Stem Cell Research

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Study Findings from National Institutes of Health (NIH) Provide New Insights into Stem Cell Research (Facs-isolation and Culture of Fibro-adipogenic Progenitors and Muscle Stem Cells From Unperturbed and Injured Mouse Skeletal Muscle). Date: Feb. 6, 2023 From: Stem Cell Week Publisher: NewsRX LLC Document Type: Article Length: 486 words

Full Text: 2023 FEB 6 (NewsRx) -- By a News Reporter-Staff News Editor at Stem Cell Week -- Researchers detail new data in Stem Cell Research. According to news originating from Bethesda, Maryland, by NewsRx correspondents, research stated, "Fibro-adipogenic progenitor cells (FAPs) are a population of skeletal muscle-resident mesenchymal stromal cells (MSCs) capable of differentiating along fibrogenic, adipogenic, osteogenic, or chondrogenic lineage. Together with muscle stem cells (MuSCs), FAPs play a critical role in muscle homeostasis, repair, and regeneration, while actively maintaining and remodeling the extracellular matrix (ECM)."

Financial support for this research came from National Institutes of Health (NIH) - USA.

Our news journalists obtained a quote from the research from the National Institutes of Health (NIH), "In pathological conditions, such as chronic damage and muscular dystrophies, FAPs undergo aberrant activation and differentiate into collagen-producing fibroblasts and adipocytes, leading to fibrosis and intramuscular fatty infiltration. Thus, FAPs play a dual role in muscle regeneration, either by sustaining MuSC turnover and promoting tissue repair or contributing to fibrotic scar formation and ectopic fat infiltrates, which compromise the integrity and function of the skeletal muscle tissue. A proper purification of FAPs and MuSCs is a prerequisite for understanding the biological role of these cells in physiological as well as in pathological conditions. Here, we describe a standardized method for the simultaneous isolation of FAPs and MuSCs from limb muscles of adult mice using fluorescence- activated cell sorting (FACS). The protocol describes in detail the mechanical and enzymatic dissociation of mononucleated cells from whole limb muscles and injured tibialis anterior (TA) muscles. FAPs and MuSCs are subsequently isolated using a semi- automated cell sorter to obtain pure cell populations."

According to the news editors, the research concluded: "We additionally describe an optimized method for culturing quiescent and activated FAPs and MuSCs, either alone or in coculture conditions."

This research has been peer-reviewed.

For more information on this research see: Facs-isolation and Culture of Fibro-adipogenic Progenitors and Muscle Stem Cells From Unperturbed and Injured Mouse Skeletal Muscle. Journal of Visualized Experiments, 2022(184). Journal of Visualized Experiments can be contacted at: Journal of Visualized Experiments, 1 Alewife Center, Ste 200, Cambridge, MA 02140, USA.

The news correspondents report that additional information may be obtained from Giulia Riparini, Niamsd, Lab Muscle Stem Cells & Gene Regulat, National Institutes of Health (NIH), Bethesda, MD 20892, United States. Additional authors for this research include Vittorio Sartorelli and James M. Simone.

Keywords for this news article include: Bethesda, Maryland, United States, North and Central America, Stem Cell Research, National Institutes of Health (NIH).

The citation for this news report is: NewsRx. Study Findings from National Institutes of Health (NIH) Provide New Insights into Stem Cell Research (Facs-isolation and Culture of Fibro-adipogenic Progenitors and Muscle Stem Cells From Unperturbed and Injured Mouse Skeletal Muscle). Stem Cell Week. February 6, 2023; p 1523.

http://www.newsrx.com/newsletters/Stem-Cell-Week.html Source Citation (APA 7th Edition) Study Findings from National Institutes of Health (NIH) Provide New Insights into Stem Cell Research (Facs-isolation and Culture of

Fibro-adipogenic Progenitors and Muscle Stem Cells From Unperturbed and Injured Mouse Skeletal Muscle). (2023). Stem Cell Week, 1523. https://link.gale.com/apps/doc/A735942901/GPS?u=lirn50909&sid=bookmark-GPS&xid=bfd13dc9

Gale Document Number: GALE|A735942901

http://www.newsrx.com/newsletters/Stem-Cell-Week.html