Order 626741: Microbiology Staph Lab report

Materials​ ​and ​ ​methods Describe ​ ​the ​ ​staph ​ ​strip ​ ​materials​ ​and ​ ​methods Look​ ​here: http://microgenbioproducts.com/wp-content/uploads/sites/8/2016/08/Microgen-Bioproducts-Fli er-Staph-ID-LOW-REZ.pdf http://www.tpm-tpm.com.tw/downfile.php?file=products/145023165835.pdf

Gram​ ​stain ​ ​(positive),​ ​catalase ​ ​(positive)​ ​and ​ ​latex​ ​agglutination/coagulase ​ ​tests​ ​are performed ​ ​as​ ​pre-tests​ ​on ​ ​the ​ ​isolate.​ ​Colony​ ​pigmentation ​ ​is​ ​recorded ​ ​and ​ ​then ​ ​1-2 ​ ​colonies are ​ ​inoculated ​ ​into ​ ​the ​ ​suspending ​ ​medium.​ ​The ​ ​suspension ​ ​is​ ​inoculated ​ ​into ​ ​the ​ ​12 ​ ​well ​ ​test strip ​ ​and ​ ​incubated ​ ​at​ ​37°C.​ ​After​ ​24 ​ ​hours​ ​incubation ​ ​the ​ ​strips​ ​are ​ ​read ​ ​and ​ ​the ​ ​Nitrate ​ ​and PYR ​ ​reagents​ ​added.​ ​The ​ ​resulting ​ ​5 ​ ​digit​ ​numerical ​ ​code ​ ​is​ ​entered ​ ​in ​ ​to ​ ​the ​ ​software ​ ​and ​ ​an identi ​ ​cation ​ ​returned.

Blast​ ​search The ​ ​sequence ​ ​data ​ ​returned ​ ​from​ ​the ​ ​sequencing ​ ​lab ​ ​(add ​ ​trace ​ ​file ​ ​at​ ​the ​ ​end ​ ​-​ ​do ​ ​not include ​ ​in ​ ​page ​ ​count)​ ​was​ ​trimmed ​ ​of​ ​garbled ​ ​ends​ ​and ​ ​compared ​ ​by​ ​means​ ​of​ ​a ​ ​BLAST search ​ ​to ​ ​the ​ ​database ​ ​of​ ​prokaryotic​ ​16S​ ​rRNA​ ​sequences​ ​maintained ​ ​by​ ​the ​ ​National Center​ ​for​ ​Biotechnology​ ​Information ​ ​at​ ​the ​ ​NIH. Results MSA​ ​plates Pictures​ ​of​ ​the ​ ​plates,​ ​each ​ ​pic​ ​captioned ​ ​with ​ ​explanation ​ ​of​ ​which ​ ​sample ​ ​it​ ​is

List​ ​and ​ ​describe ​ ​the ​ ​result​ ​of​ ​the ​ ​staph ​ ​strip Electrophoresis Photo ​ ​of​ ​gel Describe ​ ​result Blast​ ​search The ​ ​result​ ​ID ​ ​links​ ​are ​ ​for​ ​sequences​ ​1 ​ ​and ​ ​2 ​ ​are,​ ​respectively: https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Get&RID=0FDBYFZ7014 https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Get&RID=0FP3UW8P014

The ​ ​BLAST​ ​search ​ ​returned ​ ​100% ​ ​sequence ​ ​identity​ ​over​ ​the ​ ​longest​ ​fragment​ ​to ​ ​two sequences​ ​from​ ​Staphylococcus​ ​epidermis.​ ​Other​ ​sequences​ ​with ​ ​100% ​ ​identity​ ​included ​ ​S. caprae,​ ​S.​ ​capitis,​ ​S.​ ​saccharolyticus.​ ​It​ ​returned ​ ​a ​ ​99% ​ ​identity​ ​with ​ ​S.​ ​aureus​ ​for​ ​fragment​ ​1, and ​ ​100% ​ ​with ​ ​fragment​ ​2,​ ​both ​ ​over​ ​shorter​ ​lengths.​ ​The ​ ​hit​ ​tables​ ​are ​ ​available ​ ​in spreadsheet​ ​format​ ​(attached) Discussion While ​ ​S.​ ​epidermis​ ​scores​ ​at​ ​the ​ ​top,​ ​and ​ ​16S​ ​rRNA​ ​is​ ​highly​ ​conserved,​ ​there ​ ​is​ ​perhaps​ ​not a ​ ​very​ ​high ​ ​level ​ ​of​ ​confidence ​ ​in ​ ​the ​ ​difference ​ ​between ​ ​100% ​ ​and ​ ​99% ​ ​identical. Nevertheless,​ ​the ​ ​data ​ ​is​ ​most​ ​consistent​ ​with ​ ​the ​ ​identification ​ ​of​ ​S.​ ​epidermis. References BLAST​ ​ ​PROGRAMS.​ ​Altschul,​ ​S.F.,​ ​Gish,​ ​W.,​ ​Miller,​ ​W.,​ ​Myers,​ ​E.W.​ ​&​ ​Lipman,​ ​D.J.​ ​(1990)​ ​"​Basic local​ ​alignment​ ​search​ ​tool​."​ ​J.​ ​Mol.​ ​Biol.​ ​215:403-410.​ ​PubMed. Proper​ ​citation​ ​for​ ​these​ ​if​ ​end​ ​up​ ​using​ ​them: http://microgenbioproducts.com/wp-content/uploads/sites/8/2016/08/Microgen-Bioproducts-Fli er-Staph-ID-LOW-REZ.pdf http://www.tpm-tpm.com.tw/downfile.php?file=products/145023165835.pdf