Final Lab Report for Microbiology
ThaliaRamos99
Spring2022ClassActivity1.docxActivity #1 Section: U01
Lab Title: Introduction to Staining. Student Name: Thalia Ramos Gigato
Date: 01/25/2022 Student ID: 6141550
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Staining Technique |
Species |
Objective |
Picture |
Description Cellular Morphology |
|
Simple Stain |
Yeast- crystal violet |
40x |
|
Shape: cocci Arrangement: Clustered Color: Purple Staining characteristics: Crystal Violet Gram positive |
|
Simple Stain |
Yeast- Safranin |
40x |
|
Shape: cocci Arrangement: clustered Color: pink Staining characteristics: Safranin Gram negative |
|
Gram Stain |
Escherichia coli |
40x |
|
Shape: rods Arrangement: mostly in pairs Color: pink Staining characteristics: Gram negative
|
|
Gram Stain |
Staphylococcus aureus |
40x |
|
Shape: rods Arrangement: clustered Color: purple Staining characteristics: Crystal violet, Safranin Gram positive
|
|
Gram Stain |
Bacillus megaterium (24h) |
40x |
|
Shape: rods Arrangement: clustered Color: purple Staining characteristics: Crystal violet, safranin Gram positive |
|
Gram Stain |
Bacillus megaterium (48h) |
40x |
|
Shape: rods Arrangement: clumped Color: purple Staining characteristics: Crystal violet, safranin Gram positive |
|
Gram Stain
|
Mycobacterium sp. |
40x |
|
Shape: cocci Arrangement: clustered Color: pink Staining characteristics: Gram negative |
|
Acid fast stain |
Staphylococcus aureus |
40x |
|
Shape: spherical Arrangement: clumped Color: purple/blue Staining characteristics: Gram positive |
|
Acid fast stain |
Mycobacterium sp. |
40x |
|
Shape: rod Arrangement: in chains Color: pink Staining characteristics: Carbol fuchsin, acid alcohol, methylene blue. Gram positive Note: The results aren’t consistent since the color should be purple, this might be caused by a cross-contamination. |
|
Spores Stain |
Bacillus megaterium (24h) |
40x |
|
Shape: rods Arrangement: clumped Color: purple Staining characteristics: Gram positive
|
|
Spores Stain |
Bacillus megaterium (48h) |
40x |
|
Shape: rods Arrangement: clustered Color: purple Staining characteristics: Gram positive |
