Biology

Questions

1- Cite three (3) ethical concerns around the technology developed in the Wu et al.

Article.

2- Why did these authors begin with rodent models, prior to attempting the pig human

model?

e.g.

a) Cite the experimental benefits of the rodent model.

b) Cite the experimental benefits of the pig model.

3- How was the CRISPR system used in this paper?

Cite specific figures and provide the rationale for gene modification in the context of

these figures.

4- Cite and describe evidence from the Wu et al. paper that human cells integrated into

pig embryos.

5- Design an experiment:

• My current dog Hazel is a healthy shape and size, but is anxious and fearful.

• My former dog Bosley was amicable and confident, but prone to joint

problems.

• Design an experiment top produce a dog who is a healthy shape and size, and

who is also amicable and confident.

• Assume these characteristics are genetic, and you have access to adult and

embryonic clones of both Bosley and Hazel.

You may assume you have access to all resources currently available to scientists

today.

Be specific in elucidating the application of the scientific method to your experiment:

1) Observation

2) Hypothesis

3) Predictions

4) Experiment: Independent and dependent variables, controls, uncontrolled

variables, sample size

5) Results (predicted): How would you analyze your data

6) Conclusions: predict conclusions for the various outcomes of your experiment