Unknown Project Lab Home work

Microbiology Lab Identification of Unknown Bacteria

Student Name:_____________________ Bacteria ID #: ______________________

Tests: Record the results of the tests you used to identify your unknown organism. You CANNOT list a result for every single test, only those which were used to identify your unknown organism. Skip the sections of the tests that were not necessary to run on your unknown. When recording results, all results must be recorded properly and in the same manner as shown in lab. Any test that has more than one result associated with it must have all possible results recorded.

Gram Stain Result: _______________________

Morphology Result: ______________________

Phenol Red Sugar Broth Tests: *This test is mandatory for everyone and it is the first test that you must run.

Phenol Red Glucose Broth Phenol Red Lactose Broth Results: Results:

Interpretation of Results: Interpretation of Results:

Based on my Phenol Red Glucose and Lactose results, the possible organisms are:

The next test that I will run will be:

The reason of what I will run that test is because:

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TSIA Test: *Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary.

Results:

Interpretation of Results:

Did you identify your organism? If you still haven’t identified your organism, list the possible

organisms that you may have based on the results on this test.

Which test will you run next and why?

Oxidase Test:

*Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary.

Results:

Interpretation of Results:

Did you identify your organism?

If you still haven’t identified your organism, list the possible organisms that you may have based on the results on this test.

Which test will you run next and why?

Catalase Test: *Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary.

Results:

Interpretation of Results:

Did you identify your organism?

If you still haven’t identified your organism, list the possible organisms that you may have based on the results on this test.

Which test will you run next and why?

SIM Test:

*Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary. You must record result and interpretation for S, I, and M.

Results for each test in SIM:

Interpretations for each test in SIM:

Did you identify your organism? If you still haven’t identified your organism, list the possible

organisms that you may have based on the results on this test.

Which test will you run next and why?

MR-VP Test: *Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary. You must record result and interpretation for MR and VP.

Results for each test in MR-VP:

Interpretation for each test in MR-VP:

Did you identify your organism? If you still haven’t identified your organism, list the possible

organisms that you may have based on the results on this test.

Which test will you run next and why?

Citrate Test:

*Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary.

Results:

Interpretation of Results:

Did you identify your organism? If you still haven’t identified your organism, list the possible

organisms that you may have based on the results on this test.

Which test will you run next and why?

Gelatinase Test: *Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary.

Results:

Interpretation of Results:

Did you identify your organism? If you still haven’t identified your organism, list the possible

organisms that you may have based on the results on this test.

Which test will you run next and why?

Starch Hydrolysis (Amylase) Test: *Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary.

Results:

Interpretation of Results:

Did you identify your organism? If you still haven’t identified your organism, list the possible

organisms that you may have based on the results on this test.

Which test will you run next and why?

Casease Test: *Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary.

Results:

Interpretation of Results:

Did you identify your organism? If you still haven’t identified your organism, list the possible

organisms that you may have based on the results on this test.

Which test will you run next and why?

Urease Test:

*Complete this table ONLY if you need it to identify your unknown. Points will be taken off if you fill this table and it is not necessary.

Results:

Interpretation of Results:

Did you identify your organism? If you still haven’t identified your organism, list the possible

organisms that you may have based on the results on this test.

Which test will you run next and why?

My unknown organism was identified as: __________________________ If the organism is pathogenic, describe one disease or condition, human or veterinary, that the identified organism is a pathogen for. If the organism is non-pathogenic, describe one use of it as tool in biotechnology. The research for this section should be complete and detailed in essay format and not bullet points. All sources must be cited using MLA format. You will type your information on the next page. Guidelines are as follows: Disease or condition (IF YOUR UNKNOWN IS PATHOGENIC): Full name of disease, mechanism of the disease (what does this organism do that causes a disease state in the host), toxins or cell damage description. Treatment of the disease both supportive and pharmaceutical. Include the prevalence of the disease in the United States and any programs or research that may be ongoing for the disease. Biotechnology Use (IF YOUR UNKNOWN IS NON-PATHOGENIC): What field of biotechnology is the organism being used in. List the use, what it is being used to produce and what the purpose and function of that biotech product is. Describe if it is used for industrial or pharmaceutical purposes. List the company currently using the organism and of the product is already on the market or still in research.

Essay

Citations in MLA Format