micro lab5

Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L”

Student Name: Click here to enter text. Access Code (located on the underside of the lid of your lab kit): Click here to enter text.

“Pre-Lab Questions”

1. What is a model organism, and why is Saccharomyces cerevisiae so often used as one?

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2. Research and discuss the properties of mold that make them sometimes beneficial to humans,

and sometimes potentially harmful.

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3. Parasitic helminths are a major cause of disease in undeveloped countries around the world.

Discuss the role that microbiologists can and have played in helping to reduce the number of

infections caused by parasitic helminths.

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“EXPERIMENT 1: Mold Growth on Bread and Fruit

Data Tables Table 1: Experiment 1 Growth Results

Condition Day of First Visible

Growth Extent of Growth on

Bread (Mean) Extent of Growth on

Apple (Mean)

Bright, Control Click here to enter text. Click here to enter text. Click here to enter text.

Bright, Water Click here to enter text. Click here to enter text. Click here to enter text.

Bright, Sugar Click here to enter text. Click here to enter text. Click here to enter text.

Bright, Lemon Click here to enter text. Click here to enter text. Click here to enter text.

Bright, Vinegar Click here to enter text. Click here to enter text. Click here to enter text.

Dark, Control Click here to enter text. Click here to enter text. Click here to enter text.

Dark, Water Click here to enter text. Click here to enter text. Click here to enter text.

Dark, Sugar Click here to enter text. Click here to enter text. Click here to enter text.

Dark, Lemon Click here to enter text. Click here to enter text. Click here to enter text.

Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L”

Condition Day of First Visible

Growth Extent of Growth on

Bread (Mean) Extent of Growth on

Apple (Mean)

Dark, Vinegar Click here to enter text. Click here to enter text. Click here to enter text.

Post-Lab Questions 1. Which condition produced the most mold growth? The least? The fastest? The slowest?

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2. What was the purpose of examining three pieces of bread and three pieces of apple in each

treatment?

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3. Based on your results, what conditions are most favorable for mold growth on bread? On apple?

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4. Would these conditions apply to all fungal growth?

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5. Did you notice a difference in mold growth on the bread vs the apple? If so, what do you think

might account for this difference?

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6. How would changing the type of bread (e.g. fresh from a bakery, no preservatives vs.

prepackaged with preservatives) affect the results?

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Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L”

7. How do you think changing the temperature at which the bags were incubated affected the

results?

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8. How would you test the predictions/hypotheses from Questions 6 and 7?

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9. Look up the pH of lemon juice and vinegar. Based on your results and your knowledge of

favorable environmental conditions for fungal growth, what can you conclude about the effect

of pH on growth?

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10. What is the source of the mold that grew on the samples?

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Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L”

Insert a photo of your mold growth. Include your name and access code handwritten in the

background of your photo.

“EXPERIMENT 2: Microscopic Observation of Saccharomyces Cerevisiae

Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L”

Post-Lab Questions 1. What information/structures were you able to glean from the Gram stain that you could not get

from the methylene blue stain?

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2. What information/structures were you able to glean from the methylene blue stain that you

could not get from the Gram stain?

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3. Is Saccharomyces cerevisiae Gram-positive or Gram-negative? Research and describe the

composition of yeast cell walls. How does the composition compare to the cell walls of Gram -

positive or Gram-negative bacteria?

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Insert a photo of your slide. Include your name and access code handwritten in the background of

your photo.

Lab 5 Eukaryotic Microbes, Parasitology, & Viruses BIO250L”