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Mid-Term Lab Practical Review – Group Activity

1. Match each reagent/tool with its associated technique:

a. Antibiotic filter disc

b. Depression slide

c. Nigrosin

d. Iodine

e. Inoculating loop & agar plate

f. Autoclave

g. Malachite green

h. Immersion oil

i. Bibulous paper

j. Hot water bath

_____ Sterilization

_____ Gram stains

_____ Motility testing

_____ Microscopy with the 100X objective

_____ Kirby-Bauer Test

_____ Isolation streaking

_____ Determination of thermal death time

_____ Negative staining

_____ Endospore staining

_____ Drying your prepared slide

2. Outline the steps for preparing a Gram stain, including preparing the smear.

3. You would expect a Mycobacterium species to be: (Gram +/Acid fast). (pick one)

4. Is Brownian motion the same as true motility? Explain.