correct Methods and results and do annotated bibliography. Lab Report for Bio 1 "Enzymes"

Writing a Scientific Report or Paper

Results of careful laboratory work are not useful unless they can be presented in a clear, concise manner to others

for comment and evaluation. Such presentations are usually in the form of a scientific paper published in a reputable

scientific journal. Scientific communications have many things in common, which leads to a rather standard style of

writing that allow the results and meaning of experimentation to be quickly grasped by the reader. Scientists do not

expect to read attractive, stimulating prose to obtain information from technical scientific papers. The experimental

design, results and explanation of results are what are attractive and stimulating not the cleverness of the prose. The

following discussion should be useful in helping you prepare your laboratory reports, which are scientific reports.

Read it carefully before beginning your reports. Your laboratory instructor may make additional comments. The

specific format of a scientific paper varies among journals. However, the format presented below is the most

commonly used. It is the format you must use in your scientific writing for this course.

Part I: Format of a Scientific Report

The scientific report will be composed of seven sections. Each section will have a heading immediately followed by

the text, figures or graphs. The order of the sections is: title, abstract, introduction, methods, results, discussion and

literature cited.

A) Format regulations:

• typed

• double spaced

• 10-12 font, Times New Roman

• 1 inch margins

• pages numbered

• titled sections

• untitled hypothesis

• Quotes are NOT allowed. Everything must be properly paraphrased.

• No website references are permitted as sources. No exceptions.

• Everything must be properly cited. It is considered plagiarism if it is not.

• Write in third person, past tense

The overall presentation/grammar/spelling will be evaluated. Although this is not an English class, these elements

are important to the proper communication of science. Before you turn in your final version, use the spell check

function and reread your report. You should also take the time to visit the Center for Academic Success to

participate in the Read, Write, and Cite Workshop series for additional help on writing your reports.

Note: Never write statements like the following: “My lab report is about…”, “My hypothesis is…”,

or any version of this type of statement.

(1) Title Section

Create a title that briefly conveys to the reader the purpose of the paper. The title of your report must be informative.

Many readers scan journal article titles and the decision whether or not to pursue an article is based on the

information in the title. Generally, this information includes: primary factor(s) manipulated or studied; outcome of

manipulation (the response or effects); and organism studied, if relevant. An example of an informative title would

be: "The Effect of Varying Serotonin Concentrations on Calcium Release at Synaptic Membrane in Motor Neurons

of Aplysia."

The title page must contain your name, Panther ID, lab partners’ names, title, and lab section.

(2) Abstract Section

The Abstract should be an autonomous summary of the entire report. It serves to help readers determine how

relevant the report is to their own interests. This section is brief, only one paragraph, in which the author indicates

what was done, the reasoning behind it, the results and the conclusions. It must highlight only the most important

elements of each major sections of the report (Introduction, Methods, Results, and Discussion). The scientific report

can be summarized into an abstract with four types of statements: purpose statements that are general in describing

the importance and/or goals of the research; methods statements that explain what was done and how it was done;

results statements that describe what information or data was acquired; and discussion/conclusion statements that

explain what the information or data probably means and what conclusions are drawn. Only the most important

aspects of the report should make it to the abstract.

This section should be between 200 and 250 words in length. This section should contain a clear summary of what

was demonstrated, how each part of the lab was carried out and how conclusions were reached. This section should

contain one or two purpose statements (without saying "The purpose of this experiment is..."), a complete summary

of each experiment (method statements) in a few sentences, and brief, accurate explanations of the results. The final

sentences should be the concluding statements.

(3) Introduction Section

This section should indicate why the study was done and give the reader sufficient background to understand the

report. The "why" of the study will include historical information that leads to your study and the significance of the

study to a specific discipline (such as developmental neurobiology) to which the study belongs. The reader, after

perusing the introduction, should know precisely the importance of the problem being addressed. You should write

about the questions you will be answering in this experiment. Although, the content of the introduction should start

broad and narrow in scope as the introduction proceeds, be careful not to start too broad. This could lead to

problems with the scope of the paper. Note: any historical background (that is, previous studies) must be properly

cited. This section must include 4 peer reviewed outside sources (outside of lab book and text book), of which 2

should be primary literature.

This section contains the basic background information for the lab report. Be sure to comment on what is the

significance of this study and its relation to the larger field. Give an example of why the study is significant. Your

hypothesis is used to make the prediction(s). The predictions are based on the background information that was

gathered. You should have a clear statement of the reason for performing the lab along with including the rationale

for each technique used.

• What was the purpose of each experiment? For each experiment, include questions that will be answered and the expected predictions of the results for each question. Try to include a hypothesis. (without saying

"My predictions are..." or "My hypothesis is...")

• Note: Just because you are writing a report about a lab exercise does not mean you are basing the entire report on one hypothesis. You are more than likely going to need to discuss more than one set of variables,

which would lead to more than one hypothesis or prediction.

• This section should be between 450-700 words long and smoothly flow from one topic to the next.

(4) Methods Section

A reader can evaluate the results of your study only if he or she understands the experimental design, the materials

used and the reasoning behind them. Thus, it is important to carefully outline procedures and techniques used.

Complicated procedures might be graphically outlined. Besides procedures, this section should include models or

equipment used (this should not be written like a laundry list of materials), source of chemicals (if relevant),

numbers and types of organisms used, including sex and strain sample sizes, number of times experimental

procedure was performed, and other pertinent factors. Please note: There should NEVER be a list of materials in this

or any section.

• This section is 200-350 words and is a narrative. No credit will be given for a methods section written in a bulleted format.

• Include a brief description of how each experiment was performed. There should be enough detail so the reader (experienced researcher) could repeat the experiment. This does not mean having an extensive

section on how you labeled a test tube. That is not important for the replication of the experiment. You

need to include the aspects of the methods which are crucial for replication.

• You should explain the procedural steps taken (summarize) and not create a duplicate of the instructions from the lab manual. You must write the procedures in your own words, not the manual’s or anyone else’s.

• Again, NO list of materials is permitted.

(5) Results Section

It is crucial that the outcomes of experiments are carefully organized and clearly presented. This is best

accomplished by presenting data in clearly labeled graphs and tables. What the tables and/or graphs are meant to

indicate should be clear without reference to text. However, references to each graph and table MUST be made in

the text of the Results section. Graphs and tables should be numbered in the order in which they are mentioned in

the text; that is, tables should be labeled as consecutive series (Table 1, Table 2, etc.). Each figure should have a

figure title, number, and brief caption. The section text should elaborate on the information presented on the table as

well as, summarize information presented in tables and/or graphs that will be pertinent to the discussion section.

You cannot turn in a lab report with a results section that does not have associated text explaining the incorporated

figures. Analyses must be performed on class data. Thus, the data represented in your report should be

representative of the class data, not just your group’s data. Included in this section should be sample data in the form

of a picture. This can be a picture of your groups data as representative of the type of data collected.

• All graphs and tables should be your own work! Graphs and tables should not be identical to anyone else's in the lab, including your lab partner's.

• Also include a concise detailed description, which clearly summarizes the graph or table. Include comments about the results for each experiment and control. This text is a verbal description of what the

table/graph/picture is illustrating (enough detail to be on its own).

• The text should be between 250-450 words long

• Do not interpret the results in this section. This section focuses on what you observed quantitatively and qualitatively throughout the collection of data. The discussion section will include why things happened the

way this did.

(6) Discussion Section

In brief, the Discussion section is where results are interpreted and conclusions are drawn. The significance and

interpretation of the study should be explained in this section. Specific points made in the Results section should be

discussed in light of previous studies and hypotheses. This means there needs to be cited literature in this section.

Often, new hypotheses are put forth, based on the experimental outcome. This may be included in your discussion.

The most important part of the Discussion section is establishing what the results indicate, both for the ongoing

study and for future studies. For each point made in the results, you need to discuss why things happened the way

they did. If there were errors made, then this needs to be included and in context with the interpretation of the

results. Were the questions you included in the Introduction section, answered by this study? If not, how could the

study be redesigned? Some questions that can be answered in interpreting the results in the Discussions section are:

Why are these results the same as (or different from) previously published studies? What parameters of the

experimental design were important in the expected (or unexpected) results? Are some of the results due to artifacts?

How do you know? How might the experimental design be altered to diminish artifacts? What are limitations of the

experimental design? Why are these results important in a broader context?

• This section should be approximately 500-700 words long.

• This section should include a clear discussion in relation to each hypothesis and/or prediction and should discuss what is known about the topic (reported by other researchers).

• Include a brief summary of your results with reference points to your results, and explain how you interpreted your results (why did things turn out the way they did?). Be sure to refer to the specific

illustration whenever discussing the results.

• Remember nothing is EVER proven. So the results either support or refute your original hypotheses/predictions.

• Also, explain why the control and experimental outcomes were what you expected or were not what you expected.

• Comments should also be made about problems and/or improvements for the next time. This is where you can discuss the experimental design and follow-up studies.

• This section is where you are essentially asking "why" everything/anything happened during the experiment.

(7) Literature Cited Section

This section includes the alphabetical listing of all sources of fact or theory mentioned in your paper that were not

generated by you. This will primarily include research articles, but may include review articles and texts as well.

The citations should be written using APA (6th edition) format.

• You MUST include at least six references. There should be at least 2 primary sources, 2 secondary sources, and you must include the general biology textbook, and lab manual (don’t forget to cite the methods).

• You must include 4 peer reviewed outside sources (outside of lab book and text book), of which 2 should be primary literature.

• No website references or any encyclopedia references are allowed.

• YOU MUST HAVE IN-TEXT CITATIONS!!!

• Any citation in the body of the report is put at the end of a sentence and should be done like this (Author's last name, year). There are very few exceptions to this. Do not write all of the author’s names for in-text

citations. If there is only one author, then write the name of the principle author. If there are two authors,

then you must write both names and if there are multiple authors write et al. after the name of the principle

author (Yes in italics- it's in Latin). Example: (Alberte et al., 2012)

• Anything that you or other biology students reading your report would not know off the top of your head, needs to have a citation at the end of the sentence. This includes anything looked up and used. This is

especially important in the introduction.

• Your textbook can be cited as the source of basic biological information related to the topic of the report.

• Many sentences in the introduction and discussion will have citations at the end, so make sure the content flows smoothly from beginning to end. Try not to have every sentence with a citation. Use connecting

sentences and your own ideas or summaries of the experiments where possible. Remember to put the in-

text citation before the final punctuation of the sentence.

Rules for constructing a bibliography

1. Use APA, 6th Edition Citation guide for BOTH in-text citations and your bibliography 2. References should be listed in alphabetical order 3. If a particular reference takes more than one line, then all lines (except the first) should be indented 4. Include ALL authors, never write “et al.,” when writing the full literature cited page 5. Remember to write “and” before the name of the last author, if the article or book has multiple authors. 6. References should not be numbered nor bulleted. 7. Everything counts including but not limited to the placement of period, italics, parentheses, etc. 8. If there are no in-text citations and no works cited section the report will be considered plagiarized.

Part II: Guidelines for BSC1010 Lab Reports

A) General Rules:

• If you are not present in lab you cannot turn-in a lab report for that lab.

• You need to be an active participant in all labs which contain experiments for the lab report. You will clean up your bench area before you leave the lab.

• You will be present (or have a make-up) for the lab. Refer to the syllabus for the make-up policy.

• You will turn in your lab report in its entirety. Turning in one or more components or sections of the lab report after the due date and time will make the entire lab report late.

• This lab report must be your own work (no plagiarism). Content used from references needs to be cited. All text and graphs should be original and not the same as your partners. You cannot resubmit work

previously done in another class (per the plagiarism policy)

• You will need to turn in only an electronic copy. Turnitin will be used to check for plagiarism. 10 points per day will be deducted from your lab report until your instructor has your report through Turnitin.

• It is your responsibility to turn your lab report on time electronically to your instructor, per their instructions. A late lab report will lose 10 points every day it is late. It is your responsibility to know how to

properly submit your report AND to check to make sure it is submitted. Even if it is a “careless little

mistake” it is a “careless little mistake” which cost you points on your report. Be absolutely sure you

submitted your report.

• The due dates have been posted since the first week of the term and are well known.

Hints for Scientific Writing: Note: Problems arise in writing scientific papers because of specific aims of scientific

writing: to be clear, concise, unambiguous, and accurate. Due to the space restrictions in journals and time

limitations of your instructor, every word must help to convey the required information. The report as a whole

should be objective and self-explanatory. The following seven recommendations should help you with your writing

for this scientific report.

1. Avoid wordiness. Eliminate redundancies. 2. Write in the past tense. You can use the present tense for conjectures in the discussion section. 3. Do not use footnotes. 4. Be sure that there is text in the Results section. Also, make sure that each graph and/or table is referred to

and that reference is not made to nonexistent tables or graphs.

5. Check that each section contains the proper information; for example, do not put results in the Methods section.

6. Check that each Literature Cited item is in the text and that each citation in the text appears in the Literature Cited section. This includes proper in-text citations.

7. Never write "My hypothesis is...", "The purpose of this experiment is..", "My predictions are..."