Lab Report

The third and final lab report is over Acid Fast Staining. This lab report needs to be typed in paragraph form (no lists or bulleted points), needs to be Times New Roman, 12 point font, double spaced and must be written with complete sentences, proper grammar, and correct spelling.

Lab Report III will be due November 1st at 12:59 PM. Late reports will be not be tolerated, as the lab was performed and results were provided to you the week PRIOR to the midterm exam.

Here is the format and information that should be covered for your lab report:

· Cover page with name, title of report, lab section, and date (No messy headers, pg numbers, etc.)

· Abstract

· Summary of paper - Should be done last, but should appear first. It should be about 5 sentences long.

· Give a brief intro. As to what you are doing- one or two sentences

· Methods you performed – not in depth, just a quick rundown about a sentence long

· Brief indication of results

· Key words – these are a few words that describe what your abstract is pertaining

· Introduction

· What is the purpose of Acid Fast Staining? What is the formal staining name? Please include general background information here.

· Who is Paul Ehrlich? Who is Frank Ziel? Who is Friedrich Neelsen? What is their significance to Acid-Fast staining? This should be included in your report.

· Detailed background info on gram-indeterminate bacteria.

· Give background information on your choice of three bacterial species of Mycobacteria (morphology, Gram response, where is it typically found, pathogenicity, etc…)

· Methods & Materials

· Info on bacteria used in the lab, staining steps, and details about the procedure

· Results

· Microscopic pictures of slides provided or retrieved from the internet (please source!)

· Reference your attached figure or figures

· Discussion

· Discuss any inconsistencies between results obtained and appropriate bacteria response

· You should attach some sort of labeled figure depiction of Acid Fast bacteria

· There should be a separate conclusion paragraph which addresses the scientific importance of Ziehl-Neelsen staining and how it is currently utilized for Mycobacterium and fungal identification.

It is NOT acceptable for you and your fellow lab students to have the same lab report. Copied work is a form of plagiarism and will not be accepted. Since labs have been moved online, you and your friends in the lab may NOT have the same lab report or work together (total opposite of social distancing, of course!).

Due to the current Covid-19 pandemic, instead of performing the Acid-Fast lab yourselves, study the PowerPoint presentation, watch the video performed by your Micro Lab GATs, read the PDF file, and watch the helpful video links. Imagine that you performed the lab with the instructions provided to you, then do your own research online about what the ideal results for Acid Fast staining. Find photos online for the lab results of the acid fast bacteria, Mycobacteria smegmatis, or use the photos provided to you by your GATs, and include them in your lab report. It should be noted in your lab report, that while you did not perform the lab yourself physically, the “following hypothetical results could and should have been observed…”, ect, ect.

Bear in mind, that EACH and EVERY photo you pull from the internet MUST be properly referenced within your lab report and on your REFERENCE PAGE! Please utilize Purdue Writing Lab – APA formatting if you have further questions in regards to proper APA scientific writing, in-text citations, ect. The link is provided below,